Simultaneous Method for the Separation and Identification of Certain Benzodiazepine Drugs in Pharmaceutical Formulations by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Benzodiazepines in complex biological matrices: Recent updates on pretreatment and detection methods

“…Commonly used sample pretreatment methods for BDZs include protein precipitation [ 21 , 22 ], filtration [ [22] , [23] , [24] , [25] ], enzymatic hydrolysis [ [26] , [27] , [28] , [29] ], direct solid-liquid extraction [ 23 , 30 , 31 ] and others. Techniques like ultrasonic-assisted extraction [ 31 , 32 ] and microwave-assisted extraction [ 33 , 34 ] have been developed to improve extraction efficiency regarding low concentrations and shortening the extraction times.…”

“…LC is a widely used analytical technique for separations based on the selectivity of components in the mixture for the stationary and mobile phases. The application of the LC method for BDZs is shown in Table S5 [ [21] , [22] , [23] , [24] , [27] , [28] , [29] , [30] , 32 , 34 , [39] , [40] , [41] , [42] , [44] , [45] , [46] , [47] , 49 , 51 , [58] , [59] , [60] , [61] , 63 , 65 , [69] , [70] , [71] , [73] , [74] , [75] , [76] , [77] , [78] , 81 , [87] , [88] , [89] , [90] , [91] , [92] , [93] , [95] , [96] , [97] , [98] , [99] , [100] , [101] , [102] , [104] , [105] , [106] , [107] , [109] , [110] , [111] , [112] , 115 , [119] , [120] , [121] , …”

Nitrogen and sulfur-doped carbon quantum dots as fluorescent nanoprobes for spectrofluorimetric determination of olanzapine and diazepam in biological fluids and dosage forms: application to content uniformity testing

“…Several reports have been published for the assay of OLZ, such as LC–MS [ 11 , 12 ], HPLC [ 13 ], GC [ 14 ], electrochemical methods [ 15 , 16 ], spectrophotometry [ 17 , 18 ], and spectrofluorimetry [ 19 , 20 ]. Similarly, several methods for the determination of DZP have been reported, such as LC–MS [ 21 , 22 ], HPLC [ 23 ], GC [ 24 ], electrochemical methods [ 25 ], spectrophotometry [ 26 , 27 ], and spectrofluorimetry [ 28 ].…”

“…In this study, the suggested spectrofluorimetric method provides an easy, rapid, and cost-effective analytical solution for the analysis of each OLZ or DZP in their pharmaceutical preparations compared to the reported methods that need tedious pretreatment steps [19] and high-cost instrumentation [21][22][23][24]. Moreover, content uniformity testing was also conducted by adopting the United States Pharmacopoeia (USP) guidelines to produce high accuracy of the developed method [29].…”