Simultaneous monitoring of action potentials and neurotransmitter release from neuron-like PC12 cells

Cui, Mei Rong; Zhao, Wei; Li, Xiang Ling; Xu, Cong Hui; Xu, Jing; Chen, Hong‐Yuan

doi:10.1016/j.aca.2019.11.074

Cited by 13 publications

(16 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The signals recorded in the experiments (Fig 2 ) were uniform with the extracellular recorded action potentials of PC12 quasi-neuronal networks reported in another published study [16], thus it could be confirmed that the recorded signals were the signals of typical triggered responses and the results proved that PC12 quasi-neuronal networks could meet the requirement of VTMM. No research has clearly reported spontaneous electrical activities of , the result of our experiments showed that the V Th of PC12 quasi-neuronal networks went up with the increase of ethanol concentration, which proved the electrical excitability of the networks went down with the increase of ethanol concentration.…”

Section: Normal V Th Of Pc12 Quasi-neuronal Networksupporting

confidence: 82%

“…These studies have proved that PC12 cells share many electrophysiological features with neurons. Some MEA-based studies on the release of neurotransmitters of PC12 cells have been reported [13][14][15], and a study on the action potentials of PC12 cells has just been published recently [16]. However, no research has been performed to compare the electrical excitability of PC12 quasi-neuronal networks with that of normal neuronal networks yet.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Study on influence of external factors on the electrical excitability of PC12 quasi-neuronal networks through Voltage Threshold Measurement Method

Lü

Meng

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

The aim of this paper was to investigate the influence of four different external factors (acetylcholine, ethanol, temperature and lidocaine hydrochloride) on PC12 quasi-neuronal networks by multielectrode-array-based Voltage Threshold Measurement Method (VTMM). At first, VTMM was employed to measure the lowest amplitude of the voltage stimulating pulses that could just trigger the action potential from PC12 quasi-neuronal networks under normal conditions, and the amplitude was defined as the normal voltage threshold (VTh). Then the changes of the VTh of PC12 quasi-neuronal networks treated by the four external factors were tested respectively. The results showed the normal VTh of PC12 quasi-neuronal networks was 36 mV. The VTh has negative correlation with the concentration of acetylcholine and has positive correlation with the concentration of ethanol. The curves of the correlation of the VTh with temperature and the concentration of lidocaine hydrochloride were U-shaped and Λ-shaped respectively. Comparing with our earlier studies on hippocampal neuronal networks and hippocampal slices, PC12 quasi-neuronal networks not only had the same typical voltage threshold characteristic, but also had similar changes on electrical excitability when treated by the four external factors mentioned above. Therefore, the rapid-formed PC12 quasi-neuronal networks could replace neuronal networks in proper conditions, and VTMM could be used to analyze the influence of external factors on the electrical excitability of PC12 quasi-neuronal networks.

show abstract

Section: Normal V Th Of Pc12 Quasi-neuronal Networksupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Study on influence of external factors on the electrical excitability of PC12 quasi-neuronal networks through Voltage Threshold Measurement Method

Lü

Meng

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Research regarding synapse formation can be conducted on PC-12 after differentiation because of the capability to form functional synapses with each other. Culturing and differentiating PC-12 cells on microelectrodes set up the chance to study in real time neurological synapses formation and function in a noninvasive way, recording simultaneous extracellular potential of neuronal cells over long periods of time [ 8 ].…”

Section: Pc-12 Cell Linementioning

confidence: 99%

“…The combination of the optical and electrical techniques enabled mapping of neuron connectivity in an entire neuronal circuit. Moreover, the real-time recording of action potential and neurotransmitter release reveals the relevance of electrical and chemical activities in the neuronal mode [ 8 ].…”

Section: Cellular Eventsmentioning

confidence: 99%

PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications

et al. 2022

View full text Add to dashboard Cite

PC-12 cells have been widely used as a neuronal line study model in many biosensing devices, mainly due to the neurogenic characteristics acquired after differentiation, such as high level of secreted neurotransmitter, neuron morphology characterized by neurite outgrowth, and expression of ion and neurotransmitter receptors. For understanding the pathophysiology processes involved in brain disorders, PC-12 cell line is extensively assessed in neuroscience research, including studies on neurotoxicity, neuroprotection, or neurosecretion. Various analytical technologies have been developed to investigate physicochemical processes and the biosensors based on optical and electrochemical techniques, among others, have been at the forefront of this development. This article summarizes the application of different biosensors in PC-12 cell cultures and presents the modern approaches employed in neuronal networks biosensing.

show abstract

“…Therefore, these cells have been widely used in nanoparticle-induced cytotoxicity and electrophysiology studies [ 15 , 16 ]. Cui et al used MEAs and fluorescence microscopy to simultaneously monitor action potentials and dopamine release from PC12 cells [ 17 ]. Our group demonstrated that the quasi-neuronal networks had similar electrical excitability changes to those in hippocampal neuronal networks and hippocampal brain slices in response to acetylcholine, ethanol, lidocaine hydrochloride, and temperature changes.…”

Section: Introductionmentioning

confidence: 99%

The cytological and electrophysiological effects of silver nanoparticles on neuron-like PC12 cells

et al. 2022

View full text Add to dashboard Cite

The aim of this study was to investigate the toxic effects and mechanism of silver nanoparticles (SNPs) on the cytological and electrophysiological properties of rat adrenal pheochromocytoma (PC12) cells. Different concentrations of SNPs (20 nm) were prepared, and the effects of different application durations on the cell viability and electrical excitability of PC12 quasi-neuronal networks were investigated. The effects of 200 μM SNPs on the neurite length, cell membrane potential (CMP) difference, intracellular Ca2+ content, mitochondrial membrane potential (MMP) difference, adenosine triphosphate (ATP) content, and reactive oxygen species (ROS) content of networks were then investigated. The results showed that 200 μM SNPs produced grade 1 cytotoxicity at 48 h of interaction, and the other concentrations of SNPs were noncytotoxic. Noncytotoxic 5 μM SNPs significantly increased electrical excitability, and noncytotoxic 100 μM SNPs led to an initial increase followed by a significant decrease in electrical excitability. Cytotoxic SNPs (200 μM) significantly decreased electrical excitability. SNPs (200 μM) led to decreases in neurite length, MMP difference and ATP content and increases in CMP difference and intracellular Ca2+ and ROS levels. The results revealed that not only cell viability but also electrophysiological properties should be considered when evaluating nanoparticle-induced neurotoxicity. The SNP-induced cytotoxicity mainly originated from its effects on ATP content, cytoskeletal structure and ROS content. The decrease in electrical excitability was mainly due to the decrease in ATP content. ATP content may thus be an important indicator of both cell viability and electrical excitability in PC12 quasi-neuronal networks.

show abstract

Simultaneous monitoring of action potentials and neurotransmitter release from neuron-like PC12 cells

Cited by 13 publications

References 42 publications

Study on influence of external factors on the electrical excitability of PC12 quasi-neuronal networks through Voltage Threshold Measurement Method

Study on influence of external factors on the electrical excitability of PC12 quasi-neuronal networks through Voltage Threshold Measurement Method

PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications

The cytological and electrophysiological effects of silver nanoparticles on neuron-like PC12 cells

Contact Info

Product

Resources

About