2001
DOI: 10.1007/s002400100203
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Simultaneous monitoring of extracellular glucose, pyruvate, and lactate in rat testies during ischemia: a microdialysis study

Abstract: A dual-probe microdialysis technique was developed for the simultaneous monitoring of glucose and related metabolite levels during testicular ischemia in rats. The determinations of lactate and pyruvate were achieved by liquid chromatography within 5 min. Glucose was determined by a microdialysis analyzer. A unilateral ligation was produced by occlusion of the right artery for 2 or 4 h in anesthetized rats. Microdialysis probes were inserted in both sides of the testis to simultaneously monitor glucose, lactat… Show more

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