Simultaneous monitoring of single cell and of micro-organ activity by PEDOT:PSS covered multi-electrode arrays

Koutsouras, Dimitrios A.; Perrier, Romain; Marquez, Ariana Villarroel; Pirog, Antoine; Pedraza, Eileen; Cloutet, Éric; Renaud, Sylvie; Raoux, Matthieu; Malliaras, George G.; Lang, Jochen

doi:10.1016/j.msec.2017.07.028

Cited by 35 publications

(49 citation statements)

References 17 publications

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“…In fact, during the multistep process of glucose metabolism, ATP is produced, which in turn controls the opening and closure of specific ion channels and triggers well-defined ion fluxes that generate an electrical current [ 145 , 146 ]. The electrical signals produced from pancreatic islets are of two types: short-lived high-frequency action potentials (AP) that reflect single cell depolarization and longer lived changes in field potentials, named “low frequency slow potentials” (SP) [ 147 ]. In particular, SPs consist of a summation of single cell activity and reflect intraislet coupling, which represents a major index of physiological quality in insulin secretion [ 148 , 149 , 150 , 151 ].…”

Section: Biosensors For Measuring Ooc’ Electrical Activitymentioning

confidence: 99%

“…Furthermore, Lebreton et al found a positive correlation between SP frequencies and glucose concentration in murine islet cells, as well as in human islets [ 148 ]. Various works in literature developed the use of extracellular recordings using MEAs to capture the electrophysiological changes of pancreatic islets, taking advantage of the unrivalled temporal resolution of these recording systems [ 147 , 148 , 150 , 152 ]. On the other hand, there are limited works combining microfluidics and MEAs to study pancreas-on-chip models.…”

Section: Biosensors For Measuring Ooc’ Electrical Activitymentioning

confidence: 99%

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Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

et al. 2020

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Organs-on-chip (OoC), often referred to as microphysiological systems (MPS), are advanced in vitro tools able to replicate essential functions of human organs. Owing to their unprecedented ability to recapitulate key features of the native cellular environments, they represent promising tools for tissue engineering and drug screening applications. The achievement of proper functionalities within OoC is crucial; to this purpose, several parameters (e.g., chemical, physical) need to be assessed. Currently, most approaches rely on off-chip analysis and imaging techniques. However, the urgent demand for continuous, noninvasive, and real-time monitoring of tissue constructs requires the direct integration of biosensors. In this review, we focus on recent strategies to miniaturize and embed biosensing systems into organs-on-chip platforms. Biosensors for monitoring biological models with metabolic activities, models with tissue barrier functions, as well as models with electromechanical properties will be described and critically evaluated. In addition, multisensor integration within multiorgan platforms will be further reviewed and discussed.

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Section: Biosensors For Measuring Ooc’ Electrical Activitymentioning

confidence: 99%

Section: Biosensors For Measuring Ooc’ Electrical Activitymentioning

confidence: 99%

Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

et al. 2020

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“…Taking the case of PEDOT derivatives, their device operation is mainly restricted to depletion mode, presenting little difference between the on/off states during switching at low bias voltages. However, a high signal-to-noise (S/N) ratio is required for signal amplification in some demanding biological applications [ 86 ]. Thus, after the first demonstration of a high-performance OECT operating in accumulation mode by McCulloch and coworkers [ 32 ], the pathway was opened to the development of new n-type materials and the elucidation of their structure–property relations for OECT applications.…”

Section: Organic Semiconductor Materials For Oectmentioning

confidence: 99%

Organic Electrochemical Transistors (OECTs) Toward Flexible and Wearable Bioelectronics

2020

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Organic electronics have emerged as a fascinating area of research and technology in the past two decades and are anticipated to replace classic inorganic semiconductors in many applications. Research on organic light-emitting diodes, organic photovoltaics, and organic thin-film transistors is already in an advanced stage, and the derived devices are commercially available. A more recent case is the organic electrochemical transistors (OECTs), whose core component is a conductive polymer in contact with ions and solvent molecules of an electrolyte, thus allowing it to simultaneously regulate electron and ion transport. OECTs are very effective in ion-to-electron transduction and sensor signal amplification. The use of synthetically tunable, biocompatible, and depositable organic materials in OECTs makes them specially interesting for biological applications and printable devices. In this review, we provide an overview of the history of OECTs, their physical characterization, and their operation mechanism. We analyze OECT performance improvements obtained by geometry design and active material selection (i.e., conductive polymers and small molecules) and conclude with their broad range of applications from biological sensors to wearable devices.

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“…Another benefit of PEDOT:PSS electrodes is the reduced thermal noise as the large interfacial capacitance decreases, because of the lower polymer cell culture media interface [53]. Also, PEDOT:PSS electrodes can be printed onto flexible and conformal substrates with improved performance that show lower noise and increased impedance in contrast to conventional MEAs with gold or titanium nitride electrodes as reported by Koutsouras et al [54] Shin et al presented single-use as well as upgraded multi-use electrochemical biosensors for time-resolved monitoring of secreted cardiac and hepatic biomarkers. [55] For automation of the microfluidic bead-based electrochemical immunosensor microvalves were integrated on-chip to allow for programmable operations of the immunoassay including immobilization of biorecognition elements, antigen binding, washing, and electrochemical sensing.…”

Section: Biosensors For Organs-on-a-chip Systems With Single Tissumentioning

confidence: 99%

Latest Trends in Biosensing for Microphysiological Organs-on-a-Chip and Body-on-a-Chip Systems

et al. 2019

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Organs-on-chips are considered next generation in vitro tools capable of recreating in vivo like, physiological-relevant microenvironments needed to cultivate 3D tissue-engineered constructs (e.g., hydrogel-based organoids and spheroids) as well as tissue barriers. These microphysiological systems are ideally suited to (a) reduce animal testing by generating human organ models, (b) facilitate drug development and (c) perform personalized medicine by integrating patient-derived cells and patient-derived induced pluripotent stem cells (iPSCs) into microfluidic devices. An important aspect of any diagnostic device and cell analysis platform, however, is the integration and application of a variety of sensing strategies to provide reliable, high-content information on the health status of the in vitro model of choice. To overcome the analytical limitations of organs-on-a-chip systems a variety of biosensors have been integrated to provide continuous data on organ-specific reactions and dynamic tissue responses. Here, we review the latest trends in biosensors fit for monitoring human physiology in organs-on-a-chip systems including optical and electrochemical biosensors.

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Simultaneous monitoring of single cell and of micro-organ activity by PEDOT:PSS covered multi-electrode arrays

Cited by 35 publications

References 17 publications

Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

Organic Electrochemical Transistors (OECTs) Toward Flexible and Wearable Bioelectronics

Latest Trends in Biosensing for Microphysiological Organs-on-a-Chip and Body-on-a-Chip Systems

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