“…At the end of the third experimental year, soils in both columns were sampled at various depths from the soil surface (5,20,40,50,60,70 and 90 cm) to analyze the microorganism community. After sampling, 1 g soil was added in 99 mL of sterile water and the mixture was shaken for 30 min (at the rate of 170 r/min) at room temperature of 25 ± 0.5 • C. One milliliter of the soil suspension was then diluted serially (ten-fold) and used in the estimation of the amount of bacteria (denitrifying bacteria, nitrifying and nitrite bacteria) and fungi by standard spread-plate dilution method, in triplicate.…”