Simultaneous, noninvasive, in vivo, continuous monitoring of hematocrit, vascular volume, hemoglobin oxygen saturation, pulse rate and breathing rate in humans and other animal models using a single light source

Dent, Paul; Tun, Sai Han; Fillioe, Seth; Deng, Bin; Satalin, Joshua; Nieman, Gary F.; Wilcox, Kailyn; Searles, Quinn; Narsipur, Sri; Goodisman, Jerry; Mostrom, James; Steinmann, Richard; Chaiken, J.

doi:10.1117/12.2290231

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…6 Finally, there are variations on the PV[O]H algorithm, e.g., involving simultaneous Raman spectra as the IE, to produce images that could relate blood presence, fluid presence, localized inflammation, or hypoxic conditions. 4 We will present Raman spectroscopic results from this study separately but presently we believe that unique internal chemical/physical analysis from spectroscopic/physical probing can be obtained without contact. We suggest that such imaging can be integrated with information or images from other devices, e.g., robotic surgical suites to guide treatment.…”

Section: Discussionmentioning

confidence: 99%

“…If the initial effects of the injury reduce blood flow into and out of the injured region, the resulting hypoxia will cause additional damage to the affected tissues. 3 4 is simultaneously sensitive to (1) the presence/absence of blood and (2) the oxygenation state of the hemoglobin and so would seem to be advantageous. Other techniques will almost certainly be applicable to addressing related issues such as blood flow, e.g., Doppler-based optical techniques or optical coherence techniques.…”

Section: Imaging and Scimentioning

confidence: 99%

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In vivo, noncontact, real-time, PV[O]H imaging of the immediate local physiological response to spinal cord injury in a rat model

et al. 2019

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We report a small exploratory study of a methodology for real-time imaging of chemical and physical changes in spinal cords in the immediate aftermath of a localized contusive injury. One hundred separate experiments involving scanning NIR images, one-dimensional, two-dimensional (2-D), and point measurements, obtained in vivo, within a 3 × 7 mm field, on spinal cords surgically exposed between T9 and T10 revealed differences between injured and healthy cords. The collected raw data, i.e., elastic and inelastic emission from the laser probed tissues, combined via the PV[O]H algorithm, allow construction of five images over the first 5 h post injury. Within the larger study, a total of 13 rats were studied using 2-D images, i.e., injured and control. A single 830-nm laser (100-μm diameter round spot) was spatially line-scanned across the cord to reveal photobleaching effects and surface profiles possibly locating a near surface longitudinal artery/vein. In separate experiments, the laser was scanned in two dimensions across the exposed cord surface relative to the injury in a specific pattern to avoid uneven photobleaching of the imaged tissue. The 2-D scanning produced elastic and inelastic emission that allowed construction of PV[O]H images that had good fidelity with the visually observed surfaces and separate line scans and suggested differences between the volume fractions of fluid and turbidity of injured and healthy cord tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Imaging and Scimentioning

confidence: 99%

In vivo, noncontact, real-time, PV[O]H imaging of the immediate local physiological response to spinal cord injury in a rat model

et al. 2019

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“…Enabled by new photonic technologies, improved measurement of long-standing vital signs e.g. pulse rate, breathing rate, blood pressure, temperature, and recently, oxygen saturation, as well as finding new measures of physiology, are both possible if not expected 2 . In the context of blood glucose by Raman spectroscopy we developed the FRD-PVOH algorithm and ultimately a standalone device to provide a volume normalizer i.e., to allow calculation of concentrations from Raman measurements in variably turbid media.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive in-vivo monitoring of plasma volume and hematocrit using the FRD-PVOH device: from cozy to cold

Porreca,

Dent,

Peterson

et al. 2024

Data Science for Photonics and Biophotonics

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The FRD-PVOH algorithm/medical device enables continuous noninvasive spectroscopic analysis and monitoring of physiology induced changes in the peripheral vasculature in vivo in humans, specifically red blood cell and plasma volume fractions. Previously, FRD-PVOH demonstrated a correlation between mean arterial pressure (MAP) and vascular plasma volume with tilt-table experiments, indicating physiological relevance. Continuous monitoring of plasma volume and hematocrit (Hct) with a total bandwidth of 0-0.3 Hz in volar side fingertip capillary beds, reveals MAP fluctuations superimposed on slower background. These MAP fluctuations isolated from slower vascular volume shifts and the cardiac pulse create a time series associated with vasodilation/vasoconstriction i.e., thermoregulation, an autonomic path to homeostasis. We consistently observe random walk dynamics i.e., the amplitudes of the fluctuations in the time domain are distributed as a Gaussian while Fourier analysis simultaneously confirms the presence of circulatory physiology in the results i.e., breathing effects and the Baroflex response. Lorenz plots indicate linear dynamics control short and long timescale fluctuations across both test subjects. ANOVA analysis of data from a small, on-going, study using a mild external thermoregulation protocol (2 supine subjects, 16 total hours of monitoring for each subject, in 8 sessions of 2 hours each) demonstrates that the number of fluctuations per unit time varies across the two subjects with 95% significance with or without the thermoregulation challenge. Also, the mean number of fluctuations per monitoring session was significantly greater for sessions that included an external thermoregulation challenge compared to those that did not, for both subjects at 95% confidence.

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Direct noninvasive, real-time observation of thermoregulation physiology: periodic fluctuations in hematocrit and vascular volume in the peripheral circulation

Fillioe

Dent

Deng

et al. 2019

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVII

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Simultaneous, noninvasive, in vivo, continuous monitoring of hematocrit, vascular volume, hemoglobin oxygen saturation, pulse rate and breathing rate in humans and other animal models using a single light source

Cited by 3 publications

References 20 publications

In vivo, noncontact, real-time, PV[O]H imaging of the immediate local physiological response to spinal cord injury in a rat model

In vivo, noncontact, real-time, PV[O]H imaging of the immediate local physiological response to spinal cord injury in a rat model

Noninvasive in-vivo monitoring of plasma volume and hematocrit using the FRD-PVOH device: from cozy to cold

Direct noninvasive, real-time observation of thermoregulation physiology: periodic fluctuations in hematocrit and vascular volume in the peripheral circulation

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