Simultaneous one-dimensional fluorescence lifetime measurements of OH and CO in premixed flames

Jönsson, Malin; Ehn, Andreas; Christensen, Magnus; Aldén, Marcus; Bood, Joakim

doi:10.1007/s00340-013-5570-7

Cited by 13 publications

(4 citation statements)

References 37 publications

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“…Combining with Förster Resonance Energy Transfer (FRET) techniques, FLIM can be a powerful "quantum ruler" to measure protein conformations and interactions [9][10][11][12]. Compared with fluorescence intensity imaging, FLIM is independent of the signal intensity and fluorophore concentrations, making FLIM a powerful quantitative imaging technique for applications in life sciences [13], medical diagnosis [14][15][16], drug developments [17][18][19], and flow diagnosis [20][21][22]. FLIM techniques can build on time-correlated single-photon counting (TCSPC) [23][24][25], time-gating [26][27][28], or streak cameras [29]; they record time-resolved fluorescence intensity profiles to extract lifetimes with a lifetime determination algorithm (LDA) [1].…”

Section: Introductionmentioning

confidence: 99%

Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays

Sapermsap

Chen

et al. 2020

Front. Phys.

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Intensity-and amplitude-weighted average lifetimes, denoted as τ I and τ A hereafter, are useful indicators for revealing Förster resonance energy transfer (FRET) or fluorescence quenching behaviors. In this work, we discussed the differences between τ I and τ A and presented several model-free lifetime determination algorithms (LDA), including the center-of-mass, phasor, and integral equation methods for fast τ I and τ A estimations. For model-based LDAs, we discussed the model-mismatch problems, and the results suggest that a bi-exponential model can well approximate a signal following a multi-exponential model. Depending on the application requirements, suggestions about the LDAs to be used are given. The instrument responses of the imaging systems were included in the analysis. We explained why only using the τ I model for FRET analysis can be misleading; both τ I and τ A models should be considered. We also proposed using τ A /τ I as a new indicator on two-photon fluorescence lifetime images, and the results show that τ A /τ I is an intuitive tool for visualizing multi-exponential decays.

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Section: Introductionmentioning

confidence: 99%

Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays

Sapermsap

Chen

et al. 2020

Front. Phys.

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“…In this context, the exceptional temporal resolution (Δt) of streak cameras (Δt<1 ps [9]) is a distinct advantage in comparison to PMTs (photomultiplier tubes; Δt ~ 1 ns) or ICCD cameras (intensified charge-coupled device, minimal; Δt ~ 200 ps). Jonsson et al employed a streak camera to capture ps-Laser Induced Fluorescence (ps-LIF) signals from laser-excited OH and CO in flames [10]. The use of the streak camera allowed for the measurement of the short decay time of both species down to nanosecond (OH) and few hundreds ps (CO) timescales.…”

Section: Introductionmentioning

confidence: 99%

Peculiarities of measuring fluorescence decay times by a streak camera for ps-TALIF experiments in reactive plasmas

Invernizzi

Duluard

Höft

et al. 2023

Meas. Sci. Technol.

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We present a detailed methodology for (i) correctly configuring a streak camera to capture raw picosecond two-photon absorption laser induced fluorescence signals (ps-TALIF) of H-atom in low- and atmospheric-pressure plasmas, and (ii) properly processing the recorded raw experimental data with a dedicated mathematical signal processing method to infer actual ps-TALIF signals of H-atom. The goal is the accurate determination of the decay time of the recorded ps-TALIF signals of H-atom. A ps-laser is used to excite atomic hydrogen produced in both plasmas and the raw fluorescence signals are detected by the streak camera using different time windows/ranges (TR). It is shown that the choice of the TR affects the shapes and the decay times of the recorded raw TALIF signals. This is defined as the instrumental function of the streak camera and has a Gaussian profile as determined by recording the ultrafast laser pulse at different TR. To remove this instrumental distortion and extract the actual shape of the TALIF signals, the captured raw TALIF signals were fitted using the mathematical procedure developed in this study, which involved an exponentially modified Gaussian function. The application of our methodology leads to more reliable measurements of hydrogen atoms decay times after respecting the following acquisition conditions: (i) the TR of the streak camera should be sufficiently large to capture the complete (raw) TALIF signal, and (ii) the time width of the instrumental function of the streak camera should be as small as possible compared to the actual decay time of the fluorescence, while ensuring an optimal signal-to-noise ratio. This work demonstrates the remarkable potential of the combination of ps-TALIF and streak cameras in state-of-the-art optical plasma diagnostics.

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“…Fluorescence lifetime imaging (FLIM) [1, 2] is a powerful imaging technique used in life sciences [3][4][5] and flow diagnosis areas [6][7][8][9] to observe the microenvironments of fluorescent molecules, such as pH [10,11], temperature [12,13], viscosity [14,15], or ion concentrations [16,17]. FLIM can also access protein conformational changes and protein-protein interactions through Förster Resonance Energy Transfer (FRET) processes [18,19].…”

Section: Introductionmentioning

confidence: 99%

Theoretical investigations of a modified compressed ultrafast photography method suitable for single-shot fluorescence lifetime imaging

Tian

2021

Appl. Opt.

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A single-shot fluorescence lifetime imaging (FLIM) method based on the compressed ultrafast photography (CUP) is proposed, named space-restricted CUP (srCUP). srCUP is suitable for imaging objects moving slowly (< ∼ 150/M mm/s, M is the magnification of the objective lens) in the field of view with the intensity changing within nanoseconds in a measurement window around 10 ns. We used synthetic datasets to explore the performances of srCUP compared with CUP and TCUP (a variant of CUP). srCUP not only provides superior reconstruction performances, but its reconstruction time is also two-and threefold faster than CUP and TCUP, respectively. The lifetime determination performances were assessed by estimating lifetime components, amplitude-and intensity-weighted average lifetimes (τ A and τ I ) with the reconstructed scenes using the least square method based on a bi-exponential model. srCUP has the best accuracy and precision for lifetime determinations with a relative bias less than 7% and a coefficient of variation less than 7% for τ A and a relative bias less than 10% and a coefficient of variation less than 11% for τ I .

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Simultaneous one-dimensional fluorescence lifetime measurements of OH and CO in premixed flames

Cited by 13 publications

References 37 publications

Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays

Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays

Peculiarities of measuring fluorescence decay times by a streak camera for ps-TALIF experiments in reactive plasmas

Theoretical investigations of a modified compressed ultrafast photography method suitable for single-shot fluorescence lifetime imaging

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