Simultaneous Optimization of Ultrasound-Assisted Extraction for Flavonoids and Antioxidant Activity of Angelica keiskei Using Response Surface Methodology (RSM)

Abstract

Angelica keiskei Koidzumi (A. keiskei), as a Japanese edible herbal plant, enjoys a variety of biological activities due to the presence of numerous active compounds, especially flavonoids. This study aims for the optimization of ultrasound-assisted extraction (UAE) for flavonoids in A. keiskei and their antioxidant activity by using the response surface methodology (RSM). Single-factor experiments and a four-factor three-level Box–Behnken design (BBD) were performed to explore the effects of the following parameters on flavonoid extraction and antioxidant activity evaluation: ultrasonic temperature (X1), ultrasonic time (X2), ethanol concentration (X3) and liquid–solid ratio (X4). The optimum conditions of the combination of total flavonoid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity (DPPH-RSC) and ferric-reducing antioxidant power (FRAP) were as follows: X1 = 80 °C, X2 = 4 min, X3 = 78%, X4 = 35 mL/g, respectively. The experimental results provide a theoretical basis for the extensive utilization of A. keiskei and flavonoids extraction from A. keiskei as a potential source of antioxidants.

4-Hydroxyderricin Promotes Apoptosis and Cell Cycle Arrest through Regulating PI3K/AKT/mTOR Pathway in Hepatocellular Cells

“…Angelica keiskei Koidzumi ( A. keiskei ), a traditionally healthy vegetable which is originally planted in pacific-coast islands in Japan, has been reported to contain varieties of bioactive compounds, especially chalcones [ 24 ]. It was officially recognized by the National Health Commission of China as a source of new food ingredients in 2019 after its introduction in the early 1990s, and its in-depth development and industrialization have been rapidly heating domestically ever since [ 25 , 26 , 27 , 28 ]. 4-hydroxyderricin (4-HD), as one of the most abundant chalcone in A. keiskei , exists in all parts of the plant.…”

Ultrasound-Assisted Extraction of Taxifolin, Diosmin, and Quercetin from Abies nephrolepis (Trautv.) Maxim: Kinetic and Thermodynamic Characteristics

“…High correlation could be observed between the experimental data and the predicted value with "Adj R 2 " of 0.8950. The "Adeq Precision" value of 11.019 greater than 4 could conclude that an adequate signal was obtained [48]. For the 3 flavonoids extraction procedure analysis from bark of A. nephrolepis, from the ANOVA results we observed that the model is significant with F-value of 10.95, only 0.23% chance that a "Model F-Value" this large could occur large because of noise.…”

High-level production of γ-cyclodextrin glycosyltransferase in recombinant Escherichia coli BL21 (DE3): culture medium optimization, enzymatic properties characterization, and product specificity analysis

“…Considering the various factors and their interactions in the culture medium of engineered E. coli BL21 (DE3), the development of reasonable and valid optimization methodologies is necessary to avoid the unreliable results of conventional one-dimensional research (Wang et al 2014;Ebadipour et al 2016;Wu and Ahn 2018;Zhang et al 2019). In this research, the single-factor analysis elucidated that sucrose, yeast extracts, and metal irons (K + , Mg 2+ , and Ni 2+ ) could increase bacterial growth and production of soluble γ-CGTase.…”