Simultaneous Overexpression of Citrate Synthase and Phosphoenolpyruvate Carboxylase in Leaves Augments Citrate Exclusion and Al Resistance in Transgenic Tobacco

Wang, Qifeng; Yi, Qiong; Hu, Qingquan; Zhao, Yue; Nian, Hongjuan; Li, Kunzhi; Yu, Yang; Izui, Katsura; Chen, Limei

doi:10.1007/s11105-011-0397-z

Cited by 18 publications

(11 citation statements)

References 37 publications

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“…phosphate dikinase (Trejo-Tellez et al 2010); and (v) the combination of phosphoenolpyruvate carboxylase and citrate synthase (Wang et al 2012). It should be mentioned that oxalate has also been characterized within the exudates of many species (Ma 2000;Kochian et al 2004).…”

Section: Role Of the Tca Cycle In Root Developmentmentioning

confidence: 99%

On the role of the tricarboxylic acid cycle in plant productivity

Zhang

Fernie

2018

JIPB

140

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The tricarboxylic acid (TCA) cycle is one of the canonical energy pathways of living systems, as well as being an example of a pathway in which dynamic enzyme assemblies, or metabolons, are well characterized. The role of the enzymes have been the subject of saturated transgenesis approaches, whereby the expression of the constituent enzymes were reduced or knocked out in order to ascertain their in vivo function. Some of the resultant plants exhibited improved photosynthesis and plant growth, under controlled greenhouse conditions. In addition, overexpression of the endogenous genes, or heterologous forms of a number of the enzymes, has been carried out in tomato fruit and the roots of a range of species, and in some instances improvement in fruit yield and postharvest properties and plant performance, under nutrient limitation, have been reported, respectively. Given a number of variants, in nature, we discuss possible synthetic approaches involving introducing these variants, or at least a subset of them, into plants. We additionally discuss the likely consequences of introducing synthetic metabolons, wherein certain pairs of reactions are artificially permanently assembled into plants, and speculate as to future strategies to further improve plant productivity by manipulation of the core metabolic pathway.

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Section: Role Of the Tca Cycle In Root Developmentmentioning

confidence: 99%

On the role of the tricarboxylic acid cycle in plant productivity

Zhang

Fernie

2018

JIPB

140

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“…Although exudation of these compounds from the roots is specifically induced by aluminum, other stresses, including heavy metal exposure, also stimulate this process (Ding et al 2014 ). In recent years, numerous genes related to organic acids exudation were identified, and, consequently, transformation experiments towards improved Al tolerance in plants were conducted (Wang et al 2010 , 2012 ; Zhou et al 2014 ). Increased production of organic acids may be achieved by overexpression of the gene encoding enzymes catalyzing their metabolism, such as malate dehydrogenese, citrate synthase (CS), and phosphoenolpyruvate carboxylase (PEPC) (Wang et al 2010 , 2012 ).…”

Section: Strategies For Improved Efficiency Of Phytoremediationmentioning

confidence: 99%

“…In recent years, numerous genes related to organic acids exudation were identified, and, consequently, transformation experiments towards improved Al tolerance in plants were conducted (Wang et al 2010 , 2012 ; Zhou et al 2014 ). Increased production of organic acids may be achieved by overexpression of the gene encoding enzymes catalyzing their metabolism, such as malate dehydrogenese, citrate synthase (CS), and phosphoenolpyruvate carboxylase (PEPC) (Wang et al 2010 , 2012 ). Another way may be the overexpression of genes encoding citrate transporters, such as those belonging to multidrug and toxic compound extrusion (MATE) family.…”

Section: Strategies For Improved Efficiency Of Phytoremediationmentioning

confidence: 99%

Recent strategies of increasing metal tolerance and phytoremediation potential using genetic transformation of plants

Koźmińska

Wiszniewska

Hanus-Fajerska

et al. 2018

Plant Biotechnol Rep

151

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Avoidance and reduction of soil contamination with heavy metals is one of the most serious global challenges. Nowadays, science offers us new opportunities of utilizing plants to extract toxic elements from the soil by means of phytoremediation. Plant abilities to uptake, translocate, and transform heavy metals, as well as to limit their toxicity, may be significantly enhanced via genetic engineering. This paper provides a comprehensive review of recent strategies aimed at the improvement of plant phytoremediation potential using plant transformation and employing current achievements in nuclear and cytoplasmic genome transformation. Strategies for obtaining plants suitable for effective soil clean-up and tolerant to excessive concentrations of heavy metals are critically assessed. Promising directions in genetic manipulations, such as gene silencing and cis- and intragenesis, are also discussed. Moreover, the ways of overcoming disadvantages of phytoremediation using genetic transformation approachare proposed. The knowledge gathered here could be useful for designing new research aimed at biotechnological improvement of phytoremediation efficiency.

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“…For instance, the Al-activated efflux of malate is significantly correlated with Al tolerance in wheat, while a number of plants employ Al-activated efflux of citrate or both malate and citrate (Kochian et al 2004). Overexpression of genes encoding the key enzymes of citrate or malate biosynthesis, such as mitochondrial citrate synthase and malate dehydrogenase, increases citrate or/and malate exudation and Al tolerance (de la Fuente et al 1997;Anoop et al 2003;Deng et al 2009;Wang et al 2012). In some plants, such as buckwheat (Fagopyrum esculentum Moench.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Yeast Arabinono-1,4-Lactone Oxidase Gene (ALO) Increases Tolerance to Oxidative Stress and Al Toxicity in Transgenic Tobacco Plants

Chen

Qin

et al. 2014

Plant Mol Biol Rep

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L-Ascorbic acid (AsA) is the most abundant antioxidant and a major redox buffer that regulates plant responses to environmental stresses. AsA is also a precursor of oxalate in plants, while oxalate is associated with aluminum (Al) tolerance in some plant species. To enhance AsA in plants and increase tolerance against oxidative stress and Al toxicity, the yeast D-arabinono-1,4-lactone oxidase (ALO) gene was overexpressed in transgenic tobacco plants. Overexpression of ALO promoted synthesis of AsA and oxalate in leaves, but did not affect oxalate level in roots. Compared to the wild type, transgenic plants maintained higher levels of AsA, glutathione, maximal photochemical efficiency (F v /F m ) and the actual PSII efficiency (Φ PSII ) of photosystem II (PSII), and nonphotochemical quenching (NPQ), and accumulated less H 2 O 2 and malondialdehyde (MDA) in leaves in response to methyl viologen-and high-light-induced oxidative stress. In addition, transgenic plants showed higher AsA level and fresh weight of shoot and roots and lower levels of Al, H 2 O 2 , and MDA in root tips after Al treatment; however, there is no significant difference in organic acid (citrate and oxalate) exudation between the wild-type and transgenic plants in response to Al treatment. The results suggest that AsA and oxalate could be up-regulated by overexpressing ALO. The elevated AsA increased oxidative tolerance due to scavenging of reactive oxygen species (ROS) and induction of NPQ in leaves and enhanced Al tolerance as an antioxidant for avoiding Al-triggered oxidative damages in roots.

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Simultaneous Overexpression of Citrate Synthase and Phosphoenolpyruvate Carboxylase in Leaves Augments Citrate Exclusion and Al Resistance in Transgenic Tobacco

Cited by 18 publications

References 37 publications

On the role of the tricarboxylic acid cycle in plant productivity

On the role of the tricarboxylic acid cycle in plant productivity

Recent strategies of increasing metal tolerance and phytoremediation potential using genetic transformation of plants

Overexpression of Yeast Arabinono-1,4-Lactone Oxidase Gene (ALO) Increases Tolerance to Oxidative Stress and Al Toxicity in Transgenic Tobacco Plants

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