Simultaneous pharmacokinetic assessment of phytopharmaceuticals in fenugreek extract using LC–MS/MS in Sprague–Dawley rats

Abstract

Fenugreek seeds are used in numerous marketed herbal formulations with therapeutic benefits. Some of its bioactive components such as 4-hydroxyisoleucine, trigonelline, raffinose, and pinitol are reported to possess potential therapeutic activities, such as antibacterial, antidiabetic, stomach stimulant, and anti-invasive,

Simultaneous estimation of voriconazole, moxifloxacin, and pirfenidone in rabbit lacrimal matrix using LC-MS/MS: an application to preclinical ocular pharmacokinetics

“…A number of factors, such as selectivity, sensitivity, linearity, accuracyprecision, recovery, matrix effect, carryover effect, dilution integrity, and stability under different storage conditions, were examined for method validation. 39,40 2.5.1 Selectivity and sensitivity. The examination of selectivity must show that, at the retention time (s) of the analyte or the IS in the control samples, there is/are no signicant responses attributed to interfering components.…”

“…A number of factors, such as selectivity, sensitivity, linearity, accuracy–precision, recovery, matrix effect, carryover effect, dilution integrity, and stability under different storage conditions, were examined for method validation. 39,40…”

A QbD‐led simple and sensitive RP‐UHPLC method for simultaneous determination of moxifloxacin, voriconazole, and pirfenidone: An application to pharmaceutical analysis

“…By injecting a known standard ( n = 5) and determining the percentage relative standard deviation (RSD), the precision was obtained. In this section, we calculated the QC concentration values for three different QC levels using five replicates (Agrawal et al, 2023).…”

Hesperidin: Enrichment, forced degradation, and structural elucidation of potential degradation products using spectral techniques

“…The method was partially validated for linearity, selectivity, specificity, accuracy, and precision based on ICH guidelines for analytical method development 26–29 . The selectivity and reproducibility of the method were evaluated by injecting standard solutions of HES and different degradation samples.…”

“…The stability-indicating HPLC method of HES was developed using manifold optimizations and strategies learned from the compendial The method was partially validated for linearity, selectivity, specificity, accuracy, and precision based on ICH guidelines for analytical method development. [26][27][28][29] The selectivity and reproducibility of the method were evaluated by injecting standard solutions of HES and different degradation samples. The mother stock solution (1 mg/ml) was prepared in DMSO, and subsequent standard working stocks and serial dilutions were prepared in MeOH based on requirements.…”