Simultaneous production of an exopolysaccharide and chitosan by Aspergillus quadrilineatus using response surface methodology

Bagy, M. M. K.; Nafady, Nivien A.; Hassan, Elhagag Ahmed; Hashem, Marwa

doi:10.21608/aunj.2022.138922.1012

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…Aspergillus terreus [35], Fusarium solani [36], Ganoderma lucidum [37], Penicillium commune [22], and Aspergillus quadrilineatus [38] that had similar optimum temperatures between 25ºC and 30ºC for biomass and EPSs production. This may be because the temperature has a major impact on the enzymatic activation or suppression of fungal growth and the synthesis of EPSs.…”

Section: Discussionmentioning

confidence: 99%

Optimizations of exopolysaccharide production by Fusarium nygamai strain AJTYC1 and its potential applications as an antioxidant, antimicrobial, anticancer, and emulsifier

El-Mahdy,

Mohamed,

El-Ansary

2023

BMC Microbiol

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Background Exopolysaccharides (EPSs) produced by microbes are recognized as biomacromolecules of great significance. EPSs from fungi are widely used in a variety of biotechnological fields, including medicine, bioremediation, and agriculture. Results In this study, ten fungal isolates were isolated from Kafir El-Dair, Qalubia Governorate, Egypt. Isolate 5 produced more exopolysaccharides than the other examined fungi. According to microscopic morphological traits and genetic confirmation by the 18S rRNA gene, isolate 5 was identified as Fusarium nygamai strain AJTYC1. The present study showed that Czapek’s broth media, which contains 6 g/100 ml of sucrose, 10 g/100 ml of peptone, pH 6, and 1.8 × 105 CFU/ml of inoculum size and is incubated at 30 °C for 9 days, was suitable for the production of EPSs from Fusarium nygamai strain AJTYC1 by using static conditions. Fourier transform infrared (FT-IR) was employed in the characterization of EPSs, which exhibited the presence of carboxyl groups, hydroxyl groups, carbonyl groups, and glycosidic bonds. High-performance liquid chromatography (HPLC) detected that EPSs consist of sucrose and glucose. The scavenging activity indicates that EPSs have good antioxidant activity. The partially purified exopolysaccharides produced from F. nygamai strain AJTYC1 exhibited excellent antioxidant and antimicrobial activity against gram positive, gram negative and fungal strains. The EPSs at a dose of 1000 µg/ml exhibited anticancer activity against colorectal colon cancer (HCT116), breast cancer (MCF7), and hepatocellular cancer cell lines. Moreover, EPSs is an effective emulsifier of a variety of vegetable oils, and the emulsion it produces is generally stable for up to 168 h. Conclusions The production of EPSs from F. nygamai strain AJTYC1 can be used as antioxidants, antimicrobials, anticancer, and emulsifiers.

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