Simultaneous Production of Cellulase and Reducing Sugar From Alkali-Pretreated Sugarcane Bagasse via Solid State Fermentation

Abstract

aThis study optimized alkali pretreatment of sugarcane bagasse (SCB) and investigated the potential of alkali-pretreated SCB in producing cellulase and reducing sugar by a white-rot fungus, P. sanguineus, via solid state fermentation (SSF). The fermentability of the reducing sugar produced during SSF was examined by co-culturing yeast, Saccharomyces cerevisiae, with P. sanguineus. Central composite design (CCD) was applied to optimize the pretreatment based on reducing sugar yield obtained from enzymatic hydrolysis of the pretreated SCB. The model developed from CCD fitted the data well, and the optimized conditions for alkali pretreatment were 128 °C, 0.62 M NaOH, and 30 min with a reducing sugar yield of 97.8%. The alkalipretreated SCB after washing and drying was cultivated with P. sanguineus during SSF. It was found that cellulase and reducing sugar can be produced simultaneously from this SSF system. The maximum cellulase activities determined from filter paper assay (FPase), carboxylmethylcellulase (CMCase) assay and β-glucosidase assay were 0.02 IU/mL, 0.11 IU/mL, and 0.13 IU/mL on day 8, day 3, and day 6 of cultivation, respectively. The maximum reducing sugar concentration of 19.9 mg/g pretreated SCB was obtained on day 4 of SSF. The reducing sugar produced was converted into ethanol upon the addition of yeast into the SSF system. Evidently, the reducing sugar acquired can be further utilized to produce other valuable products in subsequent processes.

The Biorefining of Saudi Arabian Waste Date: An Environmental Promising Alternative to Petrochemistry.

“…This is evident from Figure 2 in which the reducing sugar concentration was seen to be decreasing when fungus growth increased as indicated by the increasing total protein content during the course of fermentation, the same result was found with the cultivation of Pycnoporus sanguineus on alkali-pretreated sugarcane Bagasse ( [47]). …”

Transformation of Starchy Lignocellulosic Biomass to Ethanol using Ragi Tapai Synergized with Microwave Irradiation Pretreatment

“…Attributable to the dual role of ragi tapai, ethanol produced from MI-pretreated starchy lignocellulosic biomass in this study was considerably higher than those from conventional pretreated lignocellulosic biomass, e.g., 4.0 g ethanol/100 g corn fiber (Rasmussen et al 2010) and 1.48 g/100 g sugarcane bagasse (Yoon et al 2012).…”

Thermophiles: A Bio-Gadget towards Waste Reclamation through Cellulase Production

“…The ever increasing costs of fossil fuels and their greenhouse effects are creating a core demand to explore alternative cheaper and eco-friendly bio-fuels resources as a strategy for reducing global warming [1]- [3]. Ligninolytic, cellulases and hemicellulases are important industrial enzymes having numerous applications and biotechnological potential for various industries including chemicals, fuel, food, brewery and wine, animal feed, textile and laundry, pulp and paper and agriculture [5], [6].…”