Simultaneous production of DHA and squalene from Aurantiochytrium sp. grown on forest biomass hydrolysates

Patel, Alok; Rova, Ulrika; Christakopoulos, Paul; Μάτσακας, Λεωνίδας

doi:10.1186/s13068-019-1593-6

Cited by 67 publications

(46 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Medium pH was adjusted to 6.8 with 1 N HCl before sterilization. The content of artificial seawater was similar as reported in our previous studies 68 . with the 100 mL of working solution.…”

Section: Methodssupporting

confidence: 89%

Co-production of DHA and squalene by thraustochytrid from forest biomass

Patel

Liefeldt

Rova

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Omega-3 fatty acids, and specifically docosahexaenoic acid (DHA), are important and essential nutrients for human health. thraustochytrids are recognised as commercial strains for nutraceuticals production, they are group of marine oleaginous microorganisms capable of co-synthesis of DHA and other valuable carotenoids in their cellular compartment. the present study sought to optimize DHA and squalene production by the thraustochytrid Schizochytrium limacinum SR21. The highest biomass yield (0.46 g/g substrate) and lipid productivity (0.239 g/g substrate) were observed with 60 g/L of glucose, following cultivation in a bioreactor, with the DHA content to be 67.76% w/w total lipids. to reduce costs, cheaper feedstocks and simultaneous production of various value-added products for pharmaceutical or energy use should be attempted. To this end, we replaced pure glucose with organosolv-pretreated spruce hydrolysate and assessed the simultaneous production of DHA and squalene from S. limacinum SR21. After the 72 h of cultivation period in bioreactor, the maximum DHA content was observed to 66.72% w/w total lipids that was corresponded to 10.15 g/L of DHA concentration. While the highest DHA productivity was 3.38 ± 0.27 g/L/d and squalene reached a total of 933.72 ± 6.53 mg/L (16.34 ± 1.81 mg/ g CDW). In summary, we show that the co-production of DHA and squalene makes S. limacinum SR21 appropriate strain for commercial-scale production of nutraceuticals. Omega-6 (n-6) and omega-3 (n-3) are polyunsaturated fatty acids (PUFAs), whose precursors include linoleic acid and alpha-linolenic acid. They are considered essential because humans cannot synthesize them due to insufficient levels of elongases and delta-6-desaturases 1,2. Although conversion of alpha-linolenic acid into omega-3 fatty acids, such as eicosapentaenoic acid (C 20:5n−3), docosapentaenoic acid (C 22:5n−3), and docosahexaenoic acid (C 22:6n−3 , DHA) occurs in humans, it happens at a very slow rate 3 and so these fatty acids must be provided by the diet. These PUFAs have several health benefits and have been found to be very effective in the preventions and treatment of fetal diseases 4. DHA plays a major role in cell signalling and is found mainly in the brain and retina tissues 5. Additionally, it acts as an anti-inflammatory agent, a precursor of several metabolites and potent lipid mediators, and could be used to treat several cardiovascular or neurologic disorders, such as hypertension and Alzheimer's 6. Fish of the Salmonidae, Scombridae, and Clupeidae families have been the sole commercial source of DHA as their oil contains approximately 20 to 30% DHA 3. However, DHA purification and concentration from fish oil is costly and the resulting oil quality depends on species, location, and pollution 7. Furthermore, fish-derived DHA is unsuitable for vegetarians, and diminishing fish stocks together with marine pollution limit the increasing demand for DHA 7. Oils from genetically engineered plant oilseeds, such as Brassica juncea, Arabidopsis thaliana, an...

show abstract

Section: Methodssupporting

confidence: 89%

Co-production of DHA and squalene by thraustochytrid from forest biomass

Patel

Liefeldt

Rova

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…T66 (ATCC PRA-276) in heterotrophic cultivation using forest biomass hydrolysates (30 g/L glucose) in flasks. The study obtained results of cell dry weight and total lipids of 10.39 g/L and 4.98 g/L, respectively, of which 25.98% constituted of DHA [23]. In the same work, Aurantiochytrium sp.…”

Section: Oleaginous Thraustochytridsmentioning

confidence: 65%

“…In the same work, Aurantiochytrium sp. T66 was also cultivated in a bioreactor and resulted in elevated values for the cell dry weight (11.24 g/L), total lipid (5.90 g/L), and DHA content (35.76% of the total lipids) [23], enabling a better process control. These results indicate the great potential in valorizing sustainable resources for the production of DHA.…”

Section: Oleaginous Thraustochytridsmentioning

confidence: 99%

“…After accessing the fatty acid profiles, the lipid obtained from various oleaginous microorganisms was deemed not suitable for biodiesel feedstock due to the high proportion of PUFA in their total lipid content [21]. These PUFA with more than two double bonds are readily susceptible to autooxidation that makes biodiesel technically unsatisfactory, with unpleasant odor [22,23]. Many of the fatty acids can be synthesized in human bodies; however, there is a group of PUFA such as docosahexaenoic acid (DHA) and as eicosapentaenoic acid (EPA) that cannot be synthesized by human beings due to lack of some desaturases and elongases that take part in the synthesis of EPA and DHA from parent omega-3 fatty acids such as α-linolenic acid (C 18:3 n−3, ALA) [24].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

Self Cite

View full text Add to dashboard Cite

Microorganisms are known to be natural oil producers in their cellular compartments. Microorganisms that accumulate more than 20% w/w of lipids on a cell dry weight basis are considered as oleaginous microorganisms. These are capable of synthesizing vast majority of fatty acids from short hydrocarbonated chain (C6) to long hydrocarbonated chain (C36), which may be saturated (SFA), monounsaturated (MUFA), or polyunsaturated fatty acids (PUFA), depending on the presence and number of double bonds in hydrocarbonated chains. Depending on the fatty acid profile, the oils obtained from oleaginous microorganisms are utilized as feedstock for either biodiesel production or as nutraceuticals. Mainly microalgae, bacteria, and yeasts are involved in the production of biodiesel, whereas thraustochytrids, fungi, and some of the microalgae are well known to be producers of very long-chain PUFA (omega-3 fatty acids). In this review article, the type of oleaginous microorganisms and their expertise in the field of biodiesel or omega-3 fatty acids, advances in metabolic engineering tools for enhanced lipid accumulation, upstream and downstream processing of lipids, including purification of biodiesel and concentration of omega-3 fatty acids are reviewed.

show abstract

“…These microalgae belong to the stramenopilic group of thraustochytrids (alternatively classified as fungi or algae) which inhabit marine and freshwaters, but also mud and decaying mangrove leaves [181,182]. Besides squalene, thraustochytrids produce other valuable lipids such as PUFAs, and docosahexaenoic acid (DHA) in particular [183,184]. The commercial interest in thraustochytrids is based on their ability to accumulate lipids faster than any other oleaginous microorganisms [185].…”

Section: Algae and Their Compounds As Humectants And Moisturizersmentioning

confidence: 99%

Enigmatic Microalgae from Aeroterrestrial and Extreme Habitats in Cosmetics: The Potential of the Untapped Natural Sources

2020

View full text Add to dashboard Cite

With the increasing demand for natural and safe products in cosmetics, algae with their diverse and valuable bioactive compounds are gaining vital importance. Until now, cosmetics have focused mainly on the use of freshwater and marine algae. However, algae are not restricted to aquatic habitats. They are found in essentially every type of aeroterrestrial and extreme environment on the Earth. There, they have to cope with harsh ecological conditions and have developed special strategies to thrive in these inimical habitats. Although not thoroughly studied, their adaptations include protective biochemical compounds which can find their application or are already used in the field of cosmetics. With proper cultivation techniques, algae from these habitats can provide novel sources of high-value functional products for the cosmetics industry, which have the advantage of being obtained in eco-friendly and cost-effective processes. However, it has to be considered that a few aeroterrestrial and extremophilic algae can be toxin producers, and in order to ensure conformity to the safe quality standards, all new ingredients must be properly tested. The aim of the present review is to unveil the hidden and underestimated potential of the enigmatic algae of aeroterrestrial and extreme habitats for the rapidly developing modern cosmetic industries.

show abstract

Simultaneous production of DHA and squalene from Aurantiochytrium sp. grown on forest biomass hydrolysates

Cited by 67 publications

References 61 publications

Co-production of DHA and squalene by thraustochytrid from forest biomass

Co-production of DHA and squalene by thraustochytrid from forest biomass

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

Enigmatic Microalgae from Aeroterrestrial and Extreme Habitats in Cosmetics: The Potential of the Untapped Natural Sources

Contact Info

Product

Resources

About