Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

Abstract

Simultaneous production of alkaline xylanase and all seven types of pectinases by a bacterial isolate, under solid-state fermentation was checked in this study. Under optimized conditions, high concurrent production of xylanase (22,800 ± 578 IU/g substrate) and pectinase (4,832 ± 189 IU/g substrate) was achieved. The different types of pectinases produced were exo-polymethylgalacturonase (782 IU/g), endo-polymethylgalacturonase (6.42 U/g), exo-polygalacturonase (2,250 IU/g), endo-polygalacturonase (11.57 U/g), polymethylgalacturonate lyase (53.99 IU/g), polygalacturonate lyase (59.78 IU/g), and pectin esterase (5.78 IU/g). Wheat bran resulted in the highest titer of both enzymes. The maximum xylanase-pectinase yield was detected after 7 days of incubation with 2 mM MgSO 4 and 1.5 g/L K 2 HPO 4 at wheat bran to moisture ratio 1:1.5 (w/v), media to flask volume ratio 1:25, pH 7.0, temperature 37 • C, and inoculum size 15%. Xylanase was most stable at pH 8.0, retained more than 75% activity up to 24 H, whereas pectinase was most stable at pH 9.0, having full activity even after 24 H. At 45 • C, the xylanase showed 82% residual activity after 6 H of incubation. The pectinase was 97% and 61% stable up to 3 H at 50 and 55 • C, respectively. This is the first report showing the production of xylanase-pectinases by bacterium along with high titer of seven types of pectinases, suitable for industries.

A novel and cost‐effective methodology for enhanced production of industrially valuable alkaline xylano‐pectinolytic enzymes cocktail in short solid‐state fermentation cycle

“…Xylano-pectinolytic enzymes were produced under SSF conditions in 250-mL Erlenmeyer flasks containing solid substrate wheat bran (10 g) as carbon source, substrate to moisture ratio 1:1.5 (wt/vol), pH 7.0, temperature 37 C, gram flour 5% (wt/wt), inoculum size 15% (vol/vol), MgSO 4 0.08% (wt/wt), and K 2 HPO 4 0.15% (w/w). 33 After 7 days of incubation interval, the enzymes were extracted twice by shaking the media contents at 200 rpm for 20 min in the presence of extraction buffer (glycine-NaOH, 0.01 M, pH 8.5). Centrifugation was done at 10,000 rpm for 15 min in order to remove the cells and debris and the clear supernatant obtained was used as source of xylanopectinolytic enzymes concoction.…”

Efficient Extraction and Clarification of Fruit Juices Using Concurrently Produced Xylano-Pectinolytic Enzymes

“…In many studies, the use of pectinase and xylanase enzyme for clarification of fruit juices has been presented as an efficient alternative to improve yield and clarity The cost of producing enzymes is a critical factor in determining whether or not they can be applied in industries. The co-production of enzymes by a single bacterial isolate, as well as use of agricultural waste as a production substrate, can reduce production costs (Kaur et al, 2019). Concurrent production of enzymes can save energy and sustaining expenses.…”