Simultaneous production of phycobiliproteins and carbonic anhydrase by <i>Spirulina platensis</i> LEB‐52

Sala, Luisa; Ores, Joana da Costa; Moraes, Caroline Costa; Kalil, Susana Juliano

doi:10.1002/cjce.23131

Cited by 10 publications

(6 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1a). A similar result was found in previous studies in which it was demonstrated that biomass production was unchanged in nitrogen-deficient medium compared to Zarrouk medium at low light, while protein production and C-PC content were decreased (Olguín et al 2001;Sala et al 2018). When A. platensis was cultivated at high light, growth increased and resulted in a higher nitrogen demand.…”

Section: Discussionsupporting

confidence: 90%

Enhancement of C-phycocyanin productivity by Arthrospira platensis when growing on palm oil mill effluent in a two-stage semi-continuous cultivation mode

et al. 2019

View full text Add to dashboard Cite

Palm oil mill effluent (POME) is well known as agricultural wastewater that has a high potential as a medium for microalgal growth due to its high macro-and micronutrient content. The cyanobacterium Arthrospira platensis is considered as a species with a high C-phycocyanin (C-PC) content which is important for fine chemical and pharmaceutical applications. However, cultivation of A. platensis on POME to produce economically feasible amounts of C-PC has not been well explored. For this, environmental, nutritional, and cultivation modes (batch, semi-continuous) were varied to optimize C-PC productivity when cultivated at various POME concentrations. Arthrospira platensis was found to grow well on POME. Highest biomass and C-PC concentrations were found on 30-100% POME. Central composite rotatable design (CCRD) response surface methodology demonstrated that C-PC productivity was influenced by urea addition at the optimum salinity. The highest C-PC productivity was found on 100% POME during semi-continuous cultivation, while the addition of phosphorus and urea did not significantly improve C-PC productivity. By applying semi-continuous cultivation with 50% POME at the first stage and 100% POME at the second stage, a similarly high C-PC productivity (4.08 ± 1.3 mg L −1 day −1) was achieved as compared with (artificial) Zarrouk medium during batch cultivation. We conclude that, when using a two-stage semi-continuous cultivation process, A. platensis can produce economically feasible amounts of C-PC when cultivated on 100% POME.

show abstract

Section: Discussionsupporting

confidence: 90%

Enhancement of C-phycocyanin productivity by Arthrospira platensis when growing on palm oil mill effluent in a two-stage semi-continuous cultivation mode

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Zarrouk medium was used for inoculation, cultivation, and maintenance of the cyanobacterium . Cultivation was performed on discontinuous mode using a photobioreactor (Erlenmeyer flask), where 20% (v/v) of inoculum (initial concentration of 0.75 g/L) was added . Temperature, agitation/aeration, and light were performed according to Ores et al Cells were recovered by filtration through a cellulose acetate membrane and destined for the cell pretreatment step and subsequent extraction process.…”

Section: Methodsmentioning

confidence: 99%

“…In the control assay (Assay 4), wet biomass (equivalent to 5 mg of dry biomass) was resuspended directly in 10 mL of TRIS‐SO 4 buffer and UAE was carried out using a Sonic Ruptor 250 (Omni International Inc., Kennesaw, GA) with frequency of 20 kHz (potency of 60 W). During UAE, samples were kept at 4 °C and the extraction process took 12 min . All experiments were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Sala

Moraes

Kalil

2018

Biotechnology Progress

Self Cite

View full text Add to dashboard Cite

C‐phycocyanin (C‐PC) is a natural blue dye, and depending on its purity, which is measured by the ratio between the absorbance of the chromophore (A620) and the absorbance of the proteins (A280), it can be used in food (purity > 0.7), cosmetics (purity > 1.5), and therapeutic treatments (purity > 4.0). Several physical, chemical, and enzymatic methods of extraction are reported, however, few are able to extract C‐PC with purity above 0.7. An innovative method of C‐PC extraction with food grade purity from wet Spirulina platensis biomass is proposed. The cells were pretreated with ethylenediaminetetraacetic acid and subsequent C‐PC extraction was performed with tris‐(hydroxymethyl) aminomethane‐SO4 buffer. C‐PC was released after 12 h of cell pretreatment. Six variables of the extraction process were evaluated. The extraction temperature significantly influenced C‐PC extraction yield and purity. In the best condition of cell pretreatment and extraction, C‐PC with purity of 1.0 and extraction yield of 129.0 mg/g could be obtained to be used as a food dye without any purification process. Lastly, an ultrafiltration process was integrated and C‐PC was concentrated 8.8‐fold, resulting in purity of 1.6 and recovery of 93.4%.

show abstract

“…Spirulina platensis LEB-52 was cultivated in Zarrouk medium according to Sala et al (2018). The biomass was recovered by filtration and resuspended in EDTA diluted in Tris-SO4 buffer (50 mmol·L -1 , pH 7.4), with a biomass concentration of 1.5 mgbiomass·mL -1 .…”

Section: Methodsmentioning

confidence: 99%

ONE-STEP PURIFICATION OF C-PHYCOCYANIN EXTRACTED FROM THE WET BIOMASS OF Spirulina platensis LEB-52

Amarante¹,

Corrêa²,

Sala³

et al. 2018

Blucher Chemical Engineering Proceedings

View full text Add to dashboard Cite

The aim of this work was to purify C-phycocyanin (C-PC) extracted from the wet biomass of Spirulina platensis LEB-52 by ion-exchange chromatography (IEC) with elution by pH gradient and compare it with elution by step followed by NaCl gradient, which is an optimized condition for C-PC purification. The best results were obtained when pH gradient was used, resulting in C-PC with purity of 4 and 60 % of recovery in one purification step. IEC with elution by pH gradient is rarely applied in phycobiliproteins' purification, but the results showed that it is a promising technique for C-PC purification.

show abstract

Simultaneous production of phycobiliproteins and carbonic anhydrase by Spirulina platensis LEB‐52

Cited by 10 publications

References 60 publications

Enhancement of C-phycocyanin productivity by Arthrospira platensis when growing on palm oil mill effluent in a two-stage semi-continuous cultivation mode

Enhancement of C-phycocyanin productivity by Arthrospira platensis when growing on palm oil mill effluent in a two-stage semi-continuous cultivation mode

Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

ONE-STEP PURIFICATION OF C-PHYCOCYANIN EXTRACTED FROM THE WET BIOMASS OF Spirulina platensis LEB-52

Contact Info

Product

Resources

About