Simultaneous production of xylooligosaccharides and antioxidant compounds from sugarcane bagasse via enzymatic hydrolysis

Mandelli, F; Brenelli, Lívia Beatriz; Almeida, Rodrigo Ferreira de; Goldbeck, Rosana; Wolf, L. D.; Hoffmam, Zaira B.; Ruller, Roberto; Rocha, George J. M.; Mercadante, Adriana Zerlotti; Squina, Fábio M.

doi:10.1016/j.indcrop.2013.12.005

Cited by 63 publications

(21 citation statements)

References 33 publications

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“…2c).When the reaction time was further extended to 24 h, the XOS (X -X3) content increased with xylobiose as the major end product (45.66%), xylose (20.67%) and xylotriose (33.67%) suggesting the applicability of the enzyme in bioconversion of birchwoodxylan into XOS (Fig.2 d). These results were in accordance with xylanases isolated from Pleurotus ostreatus (Fathy et al, 2003), Clostridium thermocellum (Mandelli et al, 2014) and Streptomyces sp. CS624 (Mander et al, 2014).…”

Section: Xylooligosaccharides (Xos) Production From Birchwoodxylan Bysupporting

confidence: 88%

“…These results indicate that the greatest enzymatic conversion yield correlated to the lowest structural substrate complexity (Mandelli et al, 2014). Xylanase, an endolytic enzyme cleaves the backbone of xylan randomly at the places where there are two or more contiguous unsubstituted xylose residues that serve as easy access point for cleavage.…”

Section: Production Of Xylooligosaccharides (Xos) By Enzymatic Degradmentioning

confidence: 91%

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Production of antioxidant xylooligosaccharides from lignocellulosic materials using Bacillus amyloliquifaciens NRRL B-14393 xylanase

Rashad¹,

Mahmoud²,

Nooman³

et al. 2016

J App Pharm Sci

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The present study aimed to evaluate the enzymatic production of XOS, antioxidant activities together with total phenolic contents from different lignocellulosic materials and birchwoodxylan using both crude and pure forms of Bacillus amyloliquifaciens NRRL B-14393 xylanase respectively. The mode of action of the pure xylanase was studied by HPLC and the end products analysis of birchwoodxylan revealed that xylose, xylobiose and xylotriose were the only end products. The resulted XOS mixture exhibited potent antioxidant activity of 87.36 % and high total phenolic content of 182.88 mg GAE/ liter of extract. XOS were obtained from different alkali pretreated agricultural residues by the crude xylanase action with a yield in a range from 54.4% to85.5%.Maximum scavenging abilities and total phenolic contents of XOS mixtures of all the tested materials were determined. XOS mixtures of orange and mango peels revealed relatively higher antioxidant activities (96% and 76.84%, respectively) and total phenolic acid contents (156.32 and 133.74 mg GAE/ liter of extract, respectively) compared to the other XOS mixtures. In conclusion, Bacillus amyloliquifaciens NRRL B-14393 xylanase could be a promising source for production of XOS with high phenolic contents and antioxidant activities.

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Section: Xylooligosaccharides (Xos) Production From Birchwoodxylan Bysupporting

confidence: 88%

Section: Production Of Xylooligosaccharides (Xos) By Enzymatic Degradmentioning

confidence: 91%

Production of antioxidant xylooligosaccharides from lignocellulosic materials using Bacillus amyloliquifaciens NRRL B-14393 xylanase

Rashad¹,

Mahmoud²,

Nooman³

et al. 2016

J App Pharm Sci

View full text Add to dashboard Cite

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“…The cells were resuspended in lysis buffer (20 mM sodium phosphate [pH 7.5], 500 mM/L NaCl, 5 mM imidazole, 80 g/L egg lysozyme, and 5 mM polymethylsulfonyl fluoride [PMSF]) and then disrupted in an ice bath using an ultrasonic processor (seven pulses of 10 s at 500 W; VC750 Ultrasonic Processor, Sonics Vibracell). After that, the AKR from the supernatant was purified by chromatography using an AKTA FPLC system (GE Healthcare, Waukesha, WI, USA) using a 5-mL HiTrap Chelating HP column (GE Healthcare) charged with Ni 2+ followed by a Superdex 200 10/300 GL column (GE Healthcare), as previously described [68]. The concentration of purified AKR was measured using a NanoDrop 2000c instrument (Thermo Scientific, USA) and calculated using the molar extinction coefficient (37,025 1 cm −1 ).…”

Section: Methodsmentioning

confidence: 99%

The Coptotermes gestroi aldo–keto reductase: a multipurpose enzyme for biorefinery applications

Tramontina

Cairo

Liberato

et al. 2017

Biotechnol Biofuels

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BackgroundIn nature, termites can be considered as a model biological system for biofuel research based on their remarkable efficiency for lignocellulosic biomass conversion. Redox enzymes are of interest in second-generation ethanol production because they promote synergic enzymatic activity with classical hydrolases for lignocellulose saccharification and inactivate fermentation inhibitory compounds produced after lignocellulose pretreatment steps.ResultsIn the present study, the biochemical and structural characteristics of the Coptotermes gestroi aldo–keto reductase (CgAKR-1) were comprehensively investigated. CgAKR-1 displayed major structural differences compared with others AKRs, including the differences in the amino acid composition of the substrate-binding site, providing basis for classification as a founding member of a new AKR subfamily (family AKR1 I). Immunolocalization assays with anti-CgAKR-1 antibodies resulted in strong fluorescence in the salivary gland, proventriculus, and foregut. CgAKR-1 supplementation caused a 32% reduction in phenolic aldehydes, such as furfural, which act as fermentation inhibitors of hemicellulosic hydrolysates, and improved ethanol fermentation by the xylose-fermenting yeast Scheffersomyces stipitis by 45%. We observed synergistic enzymatic interactions between CgAKR-1 and commercial cellulosic cocktail for sugarcane bagasse saccharification, with a maximum synergism degree of 2.17 for sugar release. Our data indicated that additive enzymatic activity could be mediated by reactive oxygen species because CgAKR-1 could produce hydrogen peroxide.ConclusionIn summary, we identified the founding member of an AKRI subfamily with a potential role in the termite digestome. CgAKR-1 was found to be a multipurpose enzyme with potential biotechnological applications. The present work provided a basis for the development and application of integrative and multipurpose enzymes in the bioethanol production chain.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0688-6) contains supplementary material, which is available to authorized users.

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“…The results showed that xylobiose was the primary hydrolysis product, while xylopentose and xylohexose either were not produced or were degraded into smaller products. Mandelli et al (2014) evaluated the enzymatic production of XOs from sugarcane bagasse (cellulose 43.1%, hemicelluloses 25.2%, and lignin 22.9%) using a mixture of xylanase and feruloyl esterase. Samanta et al (2012) studied the production of XOs by xylanase hydrolysis from alkali solubilized xylan of natural grass (cellulose 37.25 ± 0.95%, hemicelluloses 28.10 ± 0.04%, and lignin 4.80 ± 0.30%).…”

Section: Enzymatic Hydrolysis Methodsmentioning

confidence: 99%

The Potential of Pre-Hydrolysis Liquor from the Dissolving Pulp Process as Recovery Source of Xylooligosaccharide - A Mini-Review

2016

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Simultaneous production of xylooligosaccharides and antioxidant compounds from sugarcane bagasse via enzymatic hydrolysis

Cited by 63 publications

References 33 publications

Production of antioxidant xylooligosaccharides from lignocellulosic materials using Bacillus amyloliquifaciens NRRL B-14393 xylanase

Production of antioxidant xylooligosaccharides from lignocellulosic materials using Bacillus amyloliquifaciens NRRL B-14393 xylanase

The Coptotermes gestroi aldo–keto reductase: a multipurpose enzyme for biorefinery applications

The Potential of Pre-Hydrolysis Liquor from the Dissolving Pulp Process as Recovery Source of Xylooligosaccharide - A Mini-Review

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