Simultaneous Profiling of Palmitoylomics and Glycomics with Photo/pH Dual‐Responsive Magnetic Nanocomposites

Abstract

Following an in‐depth examination of a single type of protein posttranslational modification, the synergistic analysis of two or more modification types has gradually emerged as a focal point in proteomic research. Palmitoylation and glycosylation are both critical for protein, implicated in carcinogenesis and inflammation. In this study, novel dual‐responsive magnetic nanocomposites that serve as an ideal platform for the sequential or simultaneous enrichment of palmitoyl and glycopeptides are reported. The nanocomposites denoted as magDVS‐VBA are constructed by modifying magnetic nanoparticles with azobenzene and divinyl sulfone (DVS), and self‐assembled with 4‐vinylbenzeneboronic acid (VBA)‐immobilized β‐cyclodextrin, which responds to light. The incorporated DVS component possesses the ability to recognize palmitoyl or glycopeptides under different pH conditions, whereas the introduction of VBA enhances the affinity of the nanocomposite for glycopeptides. Notably, magDVS‐VBA exhibits flexible photo‐, pH‐, and magnetic‐responsive capabilities, enabling the simultaneous recognition of hydrophobic palmitoyl peptides and hydrophilic glycopeptides for the first time. The developed platform demonstrates high specificity for sensitive palmitoylomics and glycomics analysis of mouse liver tissue, providing an effective method for studying of their crosstalk, and potential implications in clinical applications.

Cotton Ti-IMAC: Developing Phosphorylated Cotton as a Novel Platform for Phosphopeptide Enrichment

“…Given the numerous merits of the material, it is anticipated to serve as a widely applicable and robust platform for large-scale phosphopeptide enrichment, which can provide a deeper understanding of the phosphoproteome in any complex biological or clinical samples. By taking advantage of the intrinsic hydrophilicity of cotton fiber, future implementation of Cotton Ti-IMAC could also be extended to enrich other PTMs simultaneously with phosphorylation. ,, …”

“…By taking advantage of the intrinsic hydrophilicity of cotton fiber, future implementation of Cotton Ti-IMAC could also be extended to enrich other PTMs simultaneously with phosphorylation. 40,49,55 ■ ASSOCIATED CONTENT * sı Supporting Information…”

Triazine-structured covalent organic framework nanosheets with inherent hydrophilicity for the highly efficient and selective enrichment of glycosylated peptides

“…15–18 However, most of the reported boronate affinity materials have suffered from apparent disadvantages, such as relatively poor selectivity, weak affinity and alkaline pH for binding. Therefore, the HILIC-based method has occupied an important position due to its low cost, great efficiency, easy operation, excellent reproducibility, and satisfactory compatibility with MS. 19,20 Recently, various HILIC-based materials, including graphene oxides, 21,22 polymers, 23 silica particles, 24,25 magnetic nanoparticles, 26–30 and metal–organic frameworks (MOFs), 31–33 have been extensively used in the enrichment of glycopeptides. However, the relatively poor capture efficiency resulting from the low yield of immobilized hydrophilic monomers in the above-mentioned materials still limits their further applications.…”