Simultaneous quantification of 25 active constituents in the total flavonoids extract from Herba Desmodii Styracifolii by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry

Guo, Panpan; Yan, Wenying; Han, Qingjie; Wang, Chunying; Zhang, Zijian

doi:10.1002/jssc.201401360

Cited by 19 publications

(16 citation statements)

References 21 publications

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“…MRM scanning mode was used on account of the great advantage in selectivity for the quantification of analytes. In the full scan mass spectra, the quasi‐molecular ions [M+H]+ (hypaphorine and abrine) and [M‐H]‐ (schaftoside, isoschaftoside, isoliquiritigenin and luteolin) were stable and in high abundance, and most of them were in good agreement with the literature data . Thus the [M+H]+ and [M‐H]‐ ions were chosen as the precursor ions for MS/MS fragmentation analysis.…”

Section: Resultsmentioning

confidence: 78%

Simultaneous detection of four flavonoids and two alkaloids in rat plasma by LC–MS/MS and its application to a comparative study of the pharmacokinetics between Abri Herba and Abri mollis Herba extract after oral administration

Liu

Yan

Han

et al. 2019

J of Separation Science

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Abri Herba and Abri mollis Herba both were important members of the Leguminosae family in southwestern China. Abri mollis Herba was often used as Abri Herba due to their proximity, but there are few studies on pharmacokinetics to compare their main identical active compositions. A sensitive and selective high‐performance liquid chromatography with tandem mass spectrometry method in the positive/negative electrospray ionization switching mode was developed and validated for the simultaneous analysis of four flavonoids and two alkaloids in rat plasma. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of methanol and 0.5% acetic acid. The detection of the target compounds was conducted in multiple‐reaction monitoring mode with a hybrid triple quadrupole linear ion trap mass spectrometer equipped with positive/negative ion‐switching electrospray ion source. The differences in pharmacokinetics were discovered, which indicated that the substitution between them is some degree of irrationality. The validated method was successfully applied to pharmacokinetic study of the six components in male rat plasma after oral administration of Abri Herba and Abri mollis Herba extract and the results in the study would provide a useful guide for the clinical application of Abri Herba with those in Abri mollis Herba.

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Section: Resultsmentioning

confidence: 78%

Simultaneous detection of four flavonoids and two alkaloids in rat plasma by LC–MS/MS and its application to a comparative study of the pharmacokinetics between Abri Herba and Abri mollis Herba extract after oral administration

Liu

Yan

Han

et al. 2019

J of Separation Science

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“…Desmodii Styracifolii Herba, the dried aerial part of Desmodium styracifolium (Osb.) Merr is a traditional herbal medicine for treating urolithiasis in China [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…Desmodii Styracifolii Herba, the dried aerial part of Desmodium styracifolium (Osb.) Merr is a traditional herbal medicine for treating urolithiasis in China [1, 2]. The crude extracts of this herb are the main components of many Chinese herbal products such as Fufang Jinqiancao Granule, Shilintong Tablet, and the Jieshitong Capsule [3–5].…”

Section: Introductionmentioning

confidence: 99%

Comparative pharmacokinetic studies of five C‐glycosylflavones in normal and urolithiasis model rats following administration of total flavonoids from Desmodium styracifolium by liquid chromatography‐tandem mass spectrometry

Chen

Zhang

et al. 2022

J of Separation Science

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The total flavonoids of Desmodium styracifolium are the flavonoid extracts purified from Desmodii Styracifolii Herba, which has conventionally been used for treating urolithiasis in China. In this study, a sensitive and simple liquid chromatography‐tandem mass spectrometry method was developed to simultaneously determine five active components of the extracts in rat plasma. Chromatographic separation of the analytes (schaftoside, vicenin‐1, vicenin‐2, vicenin‐3, and isovitexin) was performed on an ACQUITY UPLC HSS T3 Column under gradient elution conditions. The calibration curves were linear over ranges from 0.5 to 100 ng/ml for schaftoside, vicenin‐1, vicenin‐2, and vicenin‐3, and 0.2–20 ng/ml for isovitexin. The relative standard deviation of intra‐ and inter‐day precisions were ≤6.8% and ≤8.3%, respectively, and the accuracies (relative error) were within ±7.6%. The recoveries of the analytes ranged between 97.3% and 100.3%, and the matrix effects ranged from 98.6% to 113.8%. The method was successfully applied to the pharmacokinetic studies of the five active ingredients of Desmodium styracifolium, for the first time, in both normal and urolithiasis model rats. Results revealed that the plasma levels of these components were significantly increased under the pathological state. This study provided valuable information facilitating the clinical investigation of this medicine.

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“…(Xiong et al 2015;Cheng et al 2018). It was reported that the main pharmacodynamical ingredients were flavonoids, polysaccharides and triterpenoids (Hirayama et al 1989a, b. As the major component in 21 flavonoids extracted from D. styracifolium (Guo et al 2015), the content of schaftoside was the only standard for assessing the quality of D. styracifolium (Committee 2020). It was documented that schaftoside extracted from D. styracifolium can treat cholelithiasis and urolithiasis via inhibiting cholesterol gallstone formation by activation of ileal liver X receptor α and hepatic farnesoid X receptor (Liu et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Selection and validation of reference genes for RT-qPCR analysis in Desmodium styracifolium Merr

Wang

Shi

et al. 2021

3 Biotech

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Gene expression valuated by reverse transcription-quantitative PCR (RT-qPCR) are often applied to study the gene function.To obtain accurate and reliable results, the usage of stable reference genes is essential for RT-qPCR analysis. The traditional southern Chinese medicinal herb, Desmodium styracifolium Merr is well known for its remarkable effect on the treatment of urination disturbance, urolithiasis, edema and jaundice. However, there are no ready-made reference genes identified for D. styracifolium. In this study, 13 novel genes retrieved from transcriptome datasets of four different tissues were reported according to the coefficient of variation (CV) and maximum fold change (MFC) of gene expression. The expression stability of currently used Leguminosae ACT6 was compared to the 13 candidate reference genes in different tissues and 7-day-old seedlings under different experimental conditions, which was evaluated by five statistical algorithms (geNorm/NormFinder/ BestKeeper/ΔCT/RefFinder). Our results indicated that the reference gene combinations of PP + UFM1, CCRP4 + BRM and NFD6 + NCLN1 were the most stable reference genes in leaf, stem and root tissues, respectively. The most stable reference gene combination for all tissues was CCRP4 + CUL1. In addition, the most stable reference genes for different experimental conditions were distinct, for instance SMUP1 for MeJA treatment, ERDJ2A + SMUP1 for SA treatment, NCLN1 + ERDJ2A for ABA treatment and SF3B + VAMP721d for salt stress, respectively. Our results lay a foundation for achieving accurate and reliable RT-qPCR results so as to correctly understand the function of genes in D. styracifolium.

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Simultaneous quantification of 25 active constituents in the total flavonoids extract from Herba Desmodii Styracifolii by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry

Cited by 19 publications

References 21 publications

Simultaneous detection of four flavonoids and two alkaloids in rat plasma by LC–MS/MS and its application to a comparative study of the pharmacokinetics between Abri Herba and Abri mollis Herba extract after oral administration

Simultaneous detection of four flavonoids and two alkaloids in rat plasma by LC–MS/MS and its application to a comparative study of the pharmacokinetics between Abri Herba and Abri mollis Herba extract after oral administration

Comparative pharmacokinetic studies of five C‐glycosylflavones in normal and urolithiasis model rats following administration of total flavonoids from Desmodium styracifolium by liquid chromatography‐tandem mass spectrometry

Selection and validation of reference genes for RT-qPCR analysis in Desmodium styracifolium Merr

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