Simultaneous Quantification of Ampicillin and Kanamycin in Water Samples Based on Lateral Flow Aptasensor Strip with an Internal Line

Lin, Jun; Shi, Ang; Zheng, Ziwu; Huang, Long Shuang; Wang, Yixin; Lin, Hung‐Mo; Lin, Xuexia

doi:10.3390/molecules26133806

Cited by 15 publications

(11 citation statements)

References 34 publications

(39 reference statements)

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“…Compared to antibodies, aptamers have received tremendous attention in antibiotic residue analysis because they have physicochemical stability, are low-cost, easy to synthesize in-vitro, and have simple modification procedures. Lin’s group exploited the aptasensors to achieve a quantitative ampicillin and kanamycin assay [ 25 , 26 ]. The aptasensors were designed using their aptamers and a secondary DNA fragment.…”

Section: Biosensorsmentioning

confidence: 99%

“…Furthermore, the lateral flow immunoassay (LFA) was developed to meet demands due to the addition of a sample-separation procedure during the detection process. Honggui Lin and his co-workers developed a simple and rapid method based on LFA for the detection of antibiotics by labeling fluorescent CDs and aptamers [ 25 , 26 ].…”

Section: Nanomaterial-based Optical Analysis Of Antibioticmentioning

confidence: 99%

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A Minireview for Recent Development of Nanomaterial-Based Detection of Antibiotics

Hong

Zhao

et al. 2023

Biosensors

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Antibiotics are considered a new type of organic pollutant. Antibiotic residues have become a global issue due to their harm to human health. As the use of antibiotics is increasing in human life, such as in medicine, crops, livestock, and even drinking water, the accurate analysis of antibiotics is very vital. In order to develop rapid and on-site approaches for the detection of antibiotics and the analysis of trace-level residual antibiotics, a high-sensitivity, simple, and portable solution is required. Meanwhile, the rapid nanotechnology development of a variety of nanomaterials has been achieved. In this review, nanomaterial-based techniques for antibiotic detection are discussed, and some reports that have employed combined nanomaterials with optical techniques or electrochemical techniques are highlighted.

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Section: Biosensorsmentioning

confidence: 99%

Section: Nanomaterial-based Optical Analysis Of Antibioticmentioning

confidence: 99%

A Minireview for Recent Development of Nanomaterial-Based Detection of Antibiotics

Hong

Zhao

et al. 2023

Biosensors

Self Cite

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“…Different aptamer-based sensors have been developed for this purpose. For instance, Lin et al [ 29 ] presented a rapid lateral flow assay able to simultaneously detect ampicillin (AMP) and kanamycin (KAM) in water samples after 10 min of reaction. The aptasensor worked in concentrations of AMP and KAN ranging from 0.50 to 500 ng/L and from 0.1 to 1000 ng/L, respectively, with a LOD for AMP of 0.06 and for KAM of 0.015 ng/L.…”

Section: Recent Advances In Aptamer-based Biosensorsmentioning

confidence: 99%

“…The first line is the oligonucleotides that recognize the aptamer Internal HEX-labeled DNA; the second and third line is the oligonucleotides that captured the free KAM and AMP aptamers, respectively; and the fourth line is the oligonucleotides that captured the aptamers bind to KAM and AMP. Therefore, in the presence of AMP and KAM, four lines are generated in the test section [ 29 ].…”

Section: Recent Advances In Aptamer-based Biosensorsmentioning

confidence: 99%

Detection of Emerging Pollutants Using Aptamer-Based Biosensors: Recent Advances, Challenges, and Outlook

et al. 2022

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The synergistic potentialities of innovative materials that include aptamers have opened new paradigms in biosensing platforms for high-throughput monitoring systems. The available nucleobase functional moieties in aptamers offer exclusive features for bioanalytical sensing applications. In this context, compared to various in-practice biological recognition elements, the utilization of aptamers in detection platforms results in an extensive range of advantages in terms of design flexibility, stability, and sensitivity, among other attributes. Thus, the utilization of aptamers-based biosensing platforms is extensively anticipated to meet unaddressed challenges of various in-practice and standard analytical and sensing techniques. Furthermore, the superior characteristics of aptasensors have led to their applicability in the detection of harmful pollutants present in ever-increasing concentrations in different environmental matrices and water bodies, seeking to achieve simple and real-time monitoring. Considering the above-mentioned critiques and notable functional attributes of aptamers, herein, we reviewed aptamers as a fascinating interface to design, develop, and deploy a new generation of monitoring systems to aid modern bioanalytical sensing applications. Moreover, this review aims to summarize the most recent advances in the development and application of aptasensors for the detection of various emerging pollutants (EPs), e.g., pharmaceutical, and personal care products (PPCPs), endocrine-disrupting chemicals (EDCs), pesticides and other agricultural-related compounds, and toxic heavy elements. In addition, the limitations and current challenges are also reviewed, considering the technical constraints and complexity of the environmental samples.

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“…More than 40 pharmaceuticals were authenticated in 96-well plates using a seven-sensor colorimetric array [34]. A penicillin antibiotic ampicillin was detected using colorimetric [35][36][37] and fluorimetric [38][39][40] techniques. Aggregational, enzymatic, and immune methods with colorimet-ric detection were developed for another penicillin compound, benzylpenicillin [32,41].…”

Section: Introductionmentioning

confidence: 99%

Carbocyanine-Based Fluorescent and Colorimetric Sensor Array for the Discrimination of Medicinal Compounds

et al. 2022

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Array-based optical sensing is an efficient technique for the determination and discrimination of small organic molecules. This study is aimed at the development of a simple and rapid strategy for obtaining an optical response from a wide range of low-molecular-weight organic compounds. We have suggested a colorimetric and fluorimetric sensing platform based on the combination of two response mechanisms using carbocyanine dyes: aggregation and oxidation. In the first one, the analyte forms ternary aggregates with an oppositely charged surfactant wherein the dye is solubilized in the hydrophobic domains of the surfactant accompanied with fluorescent enhancement. The second mechanism is based on the effect of the analyte on the catalytic reaction rate of dye oxidation with H2O2 in the presence of a metal ion (Cu2+, Pd2+), which entails fluorescence waning and color change. The reaction mixture in a 96-well plate is photographed in visible light (colorimetry) and the near-IR region under red light excitation (fluorimetry). In this proof-of-concept study, we demonstrated the feasibility of discrimination of nine medicinal compounds using principal component analysis: four cephalosporins (ceftriaxone, cefazolin, ceftazidime, cefotaxime), three phenothiazines (promethazine, promazine, chlorpromazine), and two penicillins (benzylpenicillin, ampicillin) in an aqueous solution and in the presence of turkey meat extract. The suggested platform allows simple and rapid recognition of analytes of various nature without using spectral equipment, except for a photo camera.

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Simultaneous Quantification of Ampicillin and Kanamycin in Water Samples Based on Lateral Flow Aptasensor Strip with an Internal Line

Cited by 15 publications

References 34 publications

A Minireview for Recent Development of Nanomaterial-Based Detection of Antibiotics

A Minireview for Recent Development of Nanomaterial-Based Detection of Antibiotics

Detection of Emerging Pollutants Using Aptamer-Based Biosensors: Recent Advances, Challenges, and Outlook

Carbocyanine-Based Fluorescent and Colorimetric Sensor Array for the Discrimination of Medicinal Compounds

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