Simultaneous quantification of caffeine and its three primary metabolites in rat plasma by liquid chromatography–tandem mass spectrometry

Choi, Eu Jin; Bae, Soo Hyeon; Park, Jung Bae; Kwon, Min Jo; Jang, Su Min; Zheng, Yin; Lee, Su-Jun; Bae, Soo Kyung

doi:10.1016/j.foodchem.2013.05.069

Cited by 43 publications

(35 citation statements)

References 24 publications

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“…First, we optimized the liquid chromatography to achieve good chromatographic separation among the isomers, caffeine, and their internal standards. Secondly, we addressed the importance of HCOOH on signal intensity and matrix effects of the analytes, which has been largely ignored by the earlier reported assays (Choi, Bae, Park, Kwon, Jang, Zheng, Lee, Lee and Bae 2013, Noh, Nepal, Jeong, Kim, Um, Seo, Kang, Park, Kang, Jeong and Jeong 2015, Noh, Oh, Nepal, Jeong, Choi, Kang, Kang, Jeong and Jeong 2016). Thirdly, we utilized isotopic analogs as internal standards ( i.e ., CAF-d9 for CAF, PAR-d3 for PAR, THY, and THM).…”

Section: Resultsmentioning

confidence: 99%

Measurement of caffeine and its three primary metabolites in human plasma by HPLC‐ESI‐MS/MS and clinical application

Chen

Parker

et al. 2017

Biomedical Chromatography

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Caffeine is a mild stimulant with significant potential for abuse being consumed in larger doses with the widespread availability of energy drinks and by novel routes of administration such as inspired powder, oral sprays, and electronic cigarettes. How these recent changes in caffeine consumption affecting caffeine disposition and abuse potential is of growing concern. In the study of caffeine disposition in humans, it is common to only measure the caffeine concentration; however, caffeine’s three major metabolites (paraxanthine, theobromine, and theophylline) retain central nervous system stimulant activity that may contribute to the overall pharmacological activity and toxicity. Therefore, it would be scientifically more rigorous to measure caffeine and its major metabolites in the evaluation of caffeine disposition in human subjects. Herein, we report a method for the simultaneous quantification of caffeine and its three major metabolites in human plasma by high-performance liquid chromatography coupled to electrospray tandem mass spectrometry (HPLC-ESI-MS/MS). Human plasma samples were treated by simple protein precipitation and the analytes were separated using a 6-min gradient program. Precision and accuracy were well within in the 15% acceptance range. The simple sample preparation, short runtime, sensitivity, and the inclusion of caffeine’s major metabolites make this assay methodology optimal for the study of caffeine’s pharmacokinetics and pharmacodynamics in human subjects.

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Section: Resultsmentioning

confidence: 99%

Measurement of caffeine and its three primary metabolites in human plasma by HPLC‐ESI‐MS/MS and clinical application

Chen

Parker

et al. 2017

Biomedical Chromatography

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show abstract

“…Plasma concentrations of caffeine and its three metabolites, paraxanthine, theobromine, and theophylline, were determined by the previous reported method with some modifications (Noh et al ., 2011; Choi et al ., 2013). Caffeine and its metabolites were analyzed using high performance liquid chromatography (1260 system, Agilent) with mass spectrometry (API-4000, AB SCIEX).…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetic Interaction of Chrysin with Caffeine in Rats

Noh

Nepal

et al. 2016

Biomolecules & Therapeutics

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Pharmacokinetic interaction of chrysin, a flavone present in honey, propolis and herbs, with caffeine was investigated in male Sprague-Dawley rats. Because chrysin inhibited CYP1A-selective ethoxyresorufin O-deethylase and methoxyresorufin O-demethylase activities in enriched rat liver microsomes, the pharmacokinetics of caffeine, a CYP 1A substrate, was studied following an intragastric administration with 100 mg/kg chrysin. In addition to the oral bioavailability of chrysin, its phase 2 metabolites, chrysin sulfate and chrysin glucuronide, were determined in rat plasma. As results, the pharmacokinetic parameters for caffeine and its three metabolites (i.e., paraxanthine, theobromine and theophylline) were not changed following chrysin treatment in vivo, despite of its inhibitory effect on CYP 1A in vitro. The bioavailability of chrysin was found to be almost zero, because chrysin was rapidly metabolized to its sulfate and glucuronide conjugates in rats. Taken together, it was concluded that the little interaction of chrysin with caffeine might be resulted from the rapid metabolism of chrysin to its phase 2 metabolites which would not have inhibitory effects on CYP enzymes responsible for caffeine metabolism.

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“…Many analytical methods have been developed for the quality control of products containing caffeine: liquid chromatography-particle beam/electron ionization mass spectrophotometry [11], liquid chromatography-tandem mass spectrometry [12], near infrared spectroscopy [13], near infra-red reflectance spectroscopy [14], gas chromatography [15] and fluorescence polarization immunoassays [16].…”

Section: Introductionmentioning

confidence: 99%

Correlation between caffeine contents of green coffee beans and altitudes of the coffee plants grown in southwest Ethiopia

Hagos¹,

Redi‐Abshiro²,

Chandravanshi³

et al. 2018

Bull. Chem. Soc. Eth.

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ABSTRACT. Caffeine contents of 45 green coffee bean samples collected from coffee plants grown at different altitudes in Southwest Ethiopia was determined by UV-Vis spectrophotometry. The caffeine contents were found in the range of 0.62 -1.2% (w/w). A moderate negative correlation (R = 0.5463) was found between the caffeine contents of green coffee beans and the altitudes at which the coffee plants were grown. The caffeine contents of 9 of the green coffee bean samples analyzed by high performance liquid chromatography (HPLC) provided comparable results in the range of 0.60−1.1% (w/w). Statistical analysis of data (t-test) indicated absence of significant differences between the caffeine contents obtained by the two methods. Nonetheless, HPLC method is precise, accurate and reliable in determining caffeine content in green coffee bean samples while the UV-Vis spectrophotometry is simple, rapid, precise and more economical.

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Simultaneous quantification of caffeine and its three primary metabolites in rat plasma by liquid chromatography–tandem mass spectrometry

Cited by 43 publications

References 24 publications

Measurement of caffeine and its three primary metabolites in human plasma by HPLC‐ESI‐MS/MS and clinical application

Measurement of caffeine and its three primary metabolites in human plasma by HPLC‐ESI‐MS/MS and clinical application

Pharmacokinetic Interaction of Chrysin with Caffeine in Rats

Correlation between caffeine contents of green coffee beans and altitudes of the coffee plants grown in southwest Ethiopia

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