Simultaneous Quantification of Ellagitannins and Related Polyphenols in Geranium thunbergii Using Quantitative NMR

Abstract

Compared to commonly employed liquid chromatography-based methods, quantitative nuclear magnetic resonance (qNMR) is a recently developed method for accurate quantification of natural compounds in extracts. The simultaneous quantification of ellagitannins and the related polyphenols of Geranium thunbergii were studied using qNMR after a short-term and long-term decoction. The qNMR fingerprint for quantifying ellagitannin was presented in this work. Geraniin was observed in the short-term decoction as a major component while corilagin was the major component of the long-term decoction. An aqueous acetone extract of G. thunbergii after long-term decoction was extracted with diethyl ether, ethyl acetate, and n-butanol. Corilagin was found as a major constituent in the ethyl acetate and n-butanol extracts. Furthermore, the contents of these polyphenols in G. thunbergii from six locations in Japan and three locations in China were quantified. The contents of geraniin and corilagin in G. thunbergii from Japan were higher than those from China. Our finding raised the possibility that qNMR can be effectively employed as a simple, accurate, and efficient method for quantification of ellagitannins in medicinal plants.

First determination of some phenolic compounds and antimicrobial activities of Geranium ibericum subsp. jubatum: A plant endemic to Turkey

“…For example, it has been noted that G. macrorrhizum has shown significant hypotensive activity in anesthetized cats [1]. In Japan, G. thunbergii has been approved as an official antidiarrheal drug [11,12].…”

Cytotoxicity screening of some Turkish plants against renal cancer cells

“…Campanula species have been reported to contain polyphenols, steroids, phenylpropanoid derivates, triterpenes, and polyacetylenes and show antioxidant activity [30,31]. Previous phytochemical studies on the Geranium genus have shown the presence of phenolic compounds such as gallic, ellagic, quinic acid, quercetin, geraniin, and corilagin [32][33]. Melampyrum arvense has been reported to exhibit antimicrobial and antioxidant activity and contain phenolic compounds [34].…”

Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition

“…In addition, we showed that GN had greater anti-influenza activity than the other constituent compounds of GHE, suggesting that GN was an important active component of GHE. Interestingly, GN is known to be hydrolyzed to corilagin, gallic acid, ellagic acid, and acid after 1 h of decoction 35 . Bastian et al .…”