Simultaneous quantification of GMP, AMP, cyclic GMP and cyclic AMP by liquid chromatography coupled to tandem mass spectrometry

Improved method for the determination of cyclic guanosine monophosphate (cGMP) in human plasma by LC–MS/MS

“…As cGMP is a very polar molecule, retention on reversed phase columns is difficult and requires a nearly 100% aqueous mobile phase [12], ion pairing [10] or reversed phase columns with hydrophilic endcapping [13]. On the other hand, porous graphitic carbon columns provide good retention behavior with polar compounds such as cGMP.…”

“…Two of the published methods [10,11] do not focus solely on cGMP, but profile many different phosphorylated nucleotides simultaneously and need therefore time-consuming chromatographic procedures. Another method based on LC-MS/MS [12] is restricted to enzyme solutions and uses no I.S. Recently, a method quantifying cGMP from human plasma utilizing an isotope labeled analogue of cGMP as I.S.…”

Liquid chromatographic methods for the determination of endogenous nucleotides and nucleotide analogs used in cancer therapy: A review

“…However an other assay using positive ESI mode for the quantification of AMP, GMP, cAMP and cyclic GMP (cGMP) in human platelet Table 3 Performance and validation parameters for the quantification of endogenous nucleotides and nucleotide analogs using UV detection mode. extracts have shown a better sensitivity than the negative ion mode [65].…”

“…The sample obtained after a protein precipitation step could be directly injected into an analytical device [42,47,57,65]. However, in some cases, the sample underwent concentration by evaporation [46,49,60] or a freeze-drying [4,56].…”

“…Their polarity increases with the number of phosphate group. Unlike nucleoside triphosphates highly polar, monophosphate nucleosides can be retained on RP-HPLC column [38,39,60,65,70]. Lu et al [71] described a RP-HPLC separation for 90 nitrogen-containing cellular metabolites including nine nucleoside monophosphates (AMP, cytidine monophosphate (CMP), UMP, guanosine monophosphate (GMP), TMP, deoxyadenosine monophosphate (dAMP), dCMP, dUMP, deoxyguanosine monophosphate (dGMP)) using a Synergi Fusion RP column (Phenomenex) and a mixture water/formic acid/methanol as mobile phase delivered by a gradient program.…”