Simultaneous quantification of multiple endogenous biothiols in single living cells by plasmonic Raman probes

Li, Shanshan; Guan, Qingsheng; Zheng, Mengmeng; Wang, Yuqi; Ye, Deju; Kang, Bin; Xu, Jing‐Juan; Chen, Hong‐Yuan

doi:10.1039/c7sc03218h

Cited by 29 publications

(17 citation statements)

References 41 publications

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“…Traditional methods to detect biothiols include high-performance liquid chromatography [19], mass spectrometry [20], and capillary electrophoresis [19,21]. However, these methods need high-cost equipment, complicated sample processing and long runtime, and are therefore not suitable for high-throughput routine clinical trials and scientific research applications [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish

Zhu

Wang

et al. 2019

Analytica Chimica Acta

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Cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play many crucial physiological roles in organisms. Their abnormal levels can cause and indicate various diseases. In the present study, a small-molecule fluorescent probe 2-(imidazo[1,2-a]pyridin-2-yl)phenyl acrylate (IPPA) was designed, synthesized and characterized by NMR, FT-IR and HRMS. IPPA can selectively detect Cys over other analytes because of an approximately 76 times enhancement in fluorescence intensity. The limit of detection of IPPA for Cys was 0.33 μM. The pseudo-first-order rate constant of the reaction between IPPA and Cys was approximately 10 times that of the reaction between IPPA and Hcy (K Cys 3.18 × 10 −3 S −1 vs K Hcy 4.92 × 10 −4 S −1), indicating that Cys can be distinguished from Hcy. In addition, IPPA exhibits strong anti-interference ability, small molecular weight, high efficiency, low toxicity and good cell permeability. It was successfully used in imaging HepG2 cells and zebrafish. The fluorescence response of IPPA for calf serum are powerful proofs for practical application. Therefore, IPPA has high potential for bioassay applications.

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Section: Introductionmentioning

confidence: 99%

A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish

Zhu

Wang

et al. 2019

Analytica Chimica Acta

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“…We designed a simple plasmon Raman probe monitoring the pH changes in the cellular microenvironment, which was composed of gold nanostars as Raman enhanced substrate, 4-MPy as Raman reporter molecules, and BSA as protective molecules, denoted as SMB (Figure A). According to the theoretical simulations in our previous work, the local electric field of gold nanostars is much stronger than gold nanospheres or nanocubes, so we chose gold nanostars to enhance the Raman signal. , “Hot spots” are mainly distributed at the tip of the gold nanostars. The gold nanostars were synthesized by a seed mediated growth method .…”

Section: Results and Discussionmentioning

confidence: 99%

“…According to the theoretical simulations in our previous work, the local electric field of gold nanostars is much stronger than gold nanospheres or nanocubes, so we chose gold nanostars to enhance the Raman signal. 25,26 "Hot spots" are mainly distributed at the tip of the gold nanostars. The gold nanostars were synthesized by a seed mediated growth method.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Monitoring the Changes of pH in Lysosomes during Autophagy and Apoptosis by Plasmon Enhanced Raman Imaging

Zhang

Wang

et al. 2019

Anal. Chem.

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Lysosomes are acidic organelles that not only participate in intracellular degradation but also relate to various cellular functions. Abnormal pH in lysosomes would lead to lysosomal dysfunction, which may further result in many diseases. In this work, we statistically analyze the pH change in the lysosomes of HeLa cells model by using surface enhanced Raman scattering (SERS) imaging technique. We prepared a plasmon Raman pH probe and localized the pH probe to lysosomes via an incubation-depletion method. The pH profiles within lysosomes during the process of cellular autophagy and apoptosis were monitored in situ by SERS imaging. The pH in lysosomes decreased slightly during the process of autophagy, while the pH in lysosomes increased during apoptosis. The phenomenon, in general, is consistent with our current biological knowledge. However, we did not observe significant variation of pH between different individual cells. This information might provide an in depth understanding about the relationship of lysosomal pH with fundamental cellular functions and mechanism of diseases.

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“…Total DOS and particulate organic S (POS) concentrations in freshwater are rarely reported due to challenges in their measurement, however, measurements of total thiol concentrations (R-SH group; 10-160 nM) indicate their potential importance in long-distance S transport (Marie et al 2015). In contrast, measurements of individual free S-containing amino acids in the water column indicate that they are only present at extremely low concentrations (0.2-5.0 nM; Horňák et al 2016), despite their presence in cells at much higher concentrations (Li et al 2017). This suggests that they may be rapidly cycled within freshwaters.…”

Section: Introductionmentioning

confidence: 99%

Rapid depletion of dissolved organic sulphur (DOS) in freshwaters

et al. 2020

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Sulphur (S) is a key macronutrient for all organisms, with similar cellular requirements to that of phosphorus (P). Studies of S cycling have often focused on the inorganic fraction, however, there is strong evidence to suggest that freshwater microorganisms may also access dissolved organic S (DOS) compounds (e.g. S-containing amino acids). The aim of this study was to compare the relative concentration and depletion rates of organic 35S-labelled amino acids (cysteine, methionine) with inorganic S (Na235SO4) in oligotrophic versus mesotrophic river waters draining from low nutrient input and moderate nutrient input land uses respectively. Our results showed that inorganic SO42− was present in the water column at much higher concentrations than free amino acids. In contrast to SO42−, however, cysteine and methionine were both rapidly depleted from the mesotrophic and oligotrophic waters with a halving time < 1 h. Only a small proportion of the DOS removed from solution was mineralized and excreted as SO42− (< 16% of the total taken up) suggesting that the DOS could be satisfying a demand for carbon (C) and S. In conclusion, even though inorganic S was abundant in freshwater, it appears that the aquatic communities retained the capacity to take up and assimilate DOS.

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Simultaneous quantification of multiple endogenous biothiols in single living cells by plasmonic Raman probes

Abstract: Three endogenous biothiols in single cells were simultaneously quantified by plasmonic Raman probes and quantitative principal component analysis (qPCA).

Cited by 29 publications

References 41 publications

A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish

A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish

Monitoring the Changes of pH in Lysosomes during Autophagy and Apoptosis by Plasmon Enhanced Raman Imaging

Rapid depletion of dissolved organic sulphur (DOS) in freshwaters

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