Simultaneous quantification of rosiglitazone and its two major metabolites, N-desmethyl and p-hydroxy rosiglitazone in human plasma by liquid chromatography/tandem mass spectrometry: Application to a pharmacokinetic study

In vitro characterization of rosiglitazone metabolites and determination of the kinetic parameters employing rat liver microsomal fraction

“…Baldwin et al obtained higher apparent V max employing human liver microsomal fraction for N-Dm-R (about 1.84 gmol/min/mg protein) compared to q-OH-R (about 0.66 gmol/min/mg protein), demonstrating higher capacity for the N-demethylation pathway, similarly to the results observed in this paper employing rat liver microsomal fraction (Baldwin et al 1999). In a pharmacokinetic study, Kim et al (2009) observed higher amounts for N-Dm-R compared to q-OH-R in human plasma.…”

Quantification of rosiglitazone in rat plasma and tissues via LC–MS/MS: Method development, validation, and application in pharmacokinetic and tissue distribution studies

“…The authors' method was linear, with an LLOQ of 0.1 ng/mL Although their method was both sensitive and selective, it was limited to only urine sample analysis (Chou et al, 2005). The reported methods describe distinct advantages, such as short run time, simple sample preparation procedure, low plasma volume requirement, and improved process sensitivity, but could not determine rosiglitazone levels in off‐target tissues such as adipose tissue, brain, bone, heart, and kidney (Chou et al, 2005; El‐Enany et al, 2012; Gonzalez et al, 2011; Kim et al, 2009; Lakshmi & Rajesh, 2010; Lin et al, 2004; Mamidi et al, 2011; Mamidi et al, 2003; Muxlow et al, 2001; O'Maille et al, 2008; Pang et al, 2009; Ramesh et al, 2015; Rashid & Ahsan, 2016). We successfully developed an LC–MS/MS system with a fast run time (5.0 min), simple sample preparation technique, and limited sample volume (200 μL), with recoveries ranging from 89.14 to 99.44% in this analysis.…”

Development and validation of a UPLC–MS/MS method for quantification of SKLB010, an investigational anti-inflammatory compound, and its application to pharmacokinetic studies in beagle dogs

“…Therefore, the optimization of mobile phase components was critical. Several mobile phases have been reported for the separation of thiazolidinediones on C18 column, such as methanol-water [16], acetonitrile-formic acid-water [17], acetonitrile-ammonium acetate-TFA-water [18], and acetonitrile-ammonium acetate-water [19]. In this work we have trialed different mobile phase combinations to achieve good resolution and symmetric peak shapes for the analyte and I.S., as well as a short run time.…”