Simultaneous quantification of three alkylated‑purine adducts in human urine using sulfonic acid poly(glycidyl methacrylate‑divinylbenzene)-based microspheres as sorbent combined with LC-MS/MS

Hu, Kai; Zhao, Guiqiu; Liu, Junwei; Jia, Lizhen; Xie, Fuwei; Zhang, Shusheng; Liu, Huimin

doi:10.1016/j.jchromb.2018.02.028

Cited by 16 publications

(13 citation statements)

References 36 publications

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“…Sample workup is simpler than for DNA, and therefore urine is a popular matrix in which to study biomarkers of exposure (Aylward et al 2017), including a wide variety of DNA adducts e.g. oxidized nucleic acid products (Broedbaek et al 2011), N -nitroso-derived DNA adducts (Da Pieve et al 2013), alkylated purines (Hu et al 2018), and aristolochic acid-derived DNA adducts (Leung and Chan 2014), illustrating the breadth of adducts in urine, which parallels that in DNA.…”

Section: Introductionmentioning

confidence: 99%

Urinary DNA adductomics – A novel approach for exposomics

Cooke

Chang

et al. 2018

Environment International

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The exposome is a concept that encompasses the totality of internal and external environmental exposures, from conception onwards. Evaluation of the exposome, across the lifecourse represents a significant challenge, e.g., methods/technology may simply not exist to comprehensively assess all exposures, or they may not be applicable to human populations, or may have insufficient sensitivity. Cellular DNA adductomics aims to determine the totality of DNA adducts in the cellular genome. However, application to human populations requires the necessarily invasive sampling of tissue, to obtain sufficient DNA for sensitive analysis, which can represent a logistical and IRB challenge, particularly when investigating vulnerable populations. To circumvent this, we recently applied DNA adductomics to urine, detecting a range of expected and unexpected 2’-deoxyribonucleoside DNA adducts. However, base excision repair, the main DNA repair pathway for non-bulky DNA adducts, and processes such as spontaneous depurination, generate nucleobase adducts. Herein we propose a strategy to simultaneously assess 2’-deoxyribonucleoside and nucleobase adducts, using a widely used mass spectrometic platform (i.e., triple quadrupole tandem mass spectrometry). This will provide a much needed DNA adductomic approach for non-invasively, biomonitoring environmental exposures, through assessing the totality of DNA adducts; contributing to the evaluation of the exposome, across the life-course.

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Section: Introductionmentioning

confidence: 99%

Urinary DNA adductomics – A novel approach for exposomics

Cooke

Chang

et al. 2018

Environment International

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“…The following sections describe these and discuss some examples of their applications. 4 on-line pharmaceuticals river, wastewater LC-UV [30] HXLPP-WCX 4 off-line pharmaceuticals river, wastewater LC-MS/MS [31] DEAEMA-DVB-SAX 12 off-line pharmaceuticals urine LC-UV [32] GMA-DVB-SCX 13 off-line alkylate purines river, wastewater LC-UV [33] 2.1. High-Capacity Polymeric Materials…”

Section: Polymer-based Materialsmentioning

confidence: 99%

“…Glycidyl methacrylate (GMA)-DVB was functionalized with sulfonic groups to display SCX properties. This material was then successfully applied to extract alkylate-purine adducts from urine samples selectively [33].…”

Section: Selective Polymeric Materialsmentioning

confidence: 99%

Materials for Solid-Phase Extraction of Organic Compounds

2019

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This review provides an overview of the most recent developments involving materials for solid-phase extraction applied to determine organic contaminants. It mainly concerns polymer-based sorbents that include high-capacity, as well as selective sorbents, inorganic-based sorbents that include those prepared using sol-gel technology along with structured porous materials based on inorganic species, and carbon nanomaterials, such as graphene and carbon nanotubes. Different types of magnetic nanoparticles coated with these materials are also reviewed. Such materials, together with their main morphological and chemical features, are described, as are some representative examples of their application as solid-phase extraction materials to extract organic compounds from different types of samples, including environmental water, biological fluids, and food.

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“…Hu et al. designed a new sulfonate functionalized poly(glycidyl methacrylate‐ co ‐divinylbenzene) stationary phase specifically for SPE of the three above mentioned alkylated‐purine adducts in human urine . High clean‐up efficiency and extraction recovery was demonstrated.…”

Section: Polymeric Mixed‐mode Stationary Phasesmentioning

confidence: 99%

“…N 3methyladenine, N 3 -ethyladenine, and N 7 -ethylguanine, are considered as potential biomarkers for DNA injury caused by exposure to cigarette smoke. Hu et al designed a new sulfonate functionalized poly(glycidyl methacrylate-codivinylbenzene) stationary phase specifically for SPE of the three above mentioned alkylated-purine adducts in human urine [80]. High clean-up efficiency and extraction recovery was demonstrated.…”

Section: Polymeric Mixed-mode Stationary Phasesmentioning

confidence: 99%

Recent advances in mixed‐mode chromatographic stationary phases

Sýkora

Řezanka

Záruba

et al. 2018

J of Separation Science

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Mixed‐mode phases have become very popular in the last decade, and the number of new mixed/multi‐mode sorbents is growing fast. Unlike single‐mode stationary phases, perfectly suited for the separation of the analytes possessing similar physicochemical properties, for instance reversed‐phase chromatography for hydrophobic solutes, mixed‐mode sorbents providing multimodal interactions can render better separation selectivity for complex mixtures of solutes differing significantly in their physicochemical characteristics. The most frequent modern mixed‐mode stationary phases are di/tri‐mode sorbents embracing the following interactions, hydrophobic, electrostatic (coulombic), and hydrophilic. According to their structures, it is possible to distinguish silica‐based, polymer‐based, hybrid, and monolithic mixed‐mode stationary phases. Herewith, newly synthesized mixed‐mode sorbents developed within the last two and half years are categorized, discussed, and summarized. The main attention is devoted to the description of the synthetic routes and characterization methods applied for the new stationary phases.

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Simultaneous quantification of three alkylated‑purine adducts in human urine using sulfonic acid poly(glycidyl methacrylate‑divinylbenzene)-based microspheres as sorbent combined with LC-MS/MS

Cited by 16 publications

References 36 publications

Urinary DNA adductomics – A novel approach for exposomics

Urinary DNA adductomics – A novel approach for exposomics

Materials for Solid-Phase Extraction of Organic Compounds

Recent advances in mixed‐mode chromatographic stationary phases

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