Simultaneous quantification of two steroidal glycosides after oral gavage of <i>Marsdenia tenacissima</i> extract in rats using a LC‐MS/MS method

Wang, Wei; Wei, Sun; Dong, Li; Qu, Xiaotong; Jin, Sha; Li, Kejun; Li, Bing; Ma, Dapeng

doi:10.1002/bmc.3326

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…Another more sensitive analytical LC-MS/MS method with one-step protein precipitation was proposed to simultaneously determine the plasma concentration of two steroidal glycosides, tenacissoside H, and tenacissoside I, in rat after oral gavage. The lower limits of quantification was 0.9 ng/mL for these two MT extracts ( 66 ). Recently, Yu et al developed a new evaluation model combining DNA barcoding techniques with thin-layer chromatography (TLC) and HPLC.…”

Section: Safety and Efficacymentioning

confidence: 98%

The Antitumor Activities of Marsdenia tenacissima

et al. 2018

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Marsdenia tenacissima (MT), a traditional Chinese herbal medicine, has long been used for thousands of years to treat asthma, tracheitis, rheumatism, etc. An increasing number of recent studies have focused on the antitumor effects of MT. The effects of MT on cancer are the result of various activated signaling pathways and inhibiting factors and the high expression levels of regulatory proteins. MT can inhibit different cancer types including non-small cell lung cancer (NSCLC), malignant tumors, hepatic carcinoma, and so on. This article mainly focuses on the activities and mechanisms of MT. In addition, the efficacy and toxicity of MT are also discussed. Further studies of MT are required for improved medicinal utilization.

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Section: Safety and Efficacymentioning

confidence: 98%

The Antitumor Activities of Marsdenia tenacissima

et al. 2018

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“…So far, several pharmacokinetic studies have been carried out on the steroids of M. tenacissima owing to their clinical applications and significant effects. For example, Wang et al quantified the tenacissoside H and I contents in rat plasma by LC–MS/MS (Wang, Sun et al, 2015). Zeng et al constructed an LC–MS/MS approach for quantifying 17 β ‐tenacigen B, tenacigenin A, tenacigenin B, tenacigenoside A, marsdenoside I and tenacigenoside F in rat plasma (Zeng et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of eight bioactive components in Marsdenia tenacissima extract in rat plasma by LC–MS/MS and its application in a pharmacokinetic study

Pei

Zhang

2020

Biomedical Chromatography

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As a traditional Chinese medicine, Marsdenia tenacissima (Roxb.) Wight et Arn. plays an indispensable role in clinical practice owing to its specific efficacy in treating malignant tumors, leukocythemia, cystitis and asthma. This study aimed to establish a novel and scientific LC-MS/MS approach to simultaneously determine tenacissoside B, H, G and I, caffeic acid, cryptochlorogenic acid, chlorogenic acid and neochlorogenic acid from M. tenacissima extract within the rat plasma samples. Digoxin was used as the internal reference. All determinations were carried out using the Eclipse Plus C 18 column, and water (containing 0.1% formic acid) was used as the mobile phase A, while acetonitrile was the mobile phase B for gradient elution. The UPLC methods were validated, including calibration curves, accuracy, precision, stability and recovery of the total eight analytes, in accordance with the requirements for biopharmaceutical analysis. Moreover, the proposed approach was also used in comprehensive pharmacokinetic research on those eight analytes in rats following M. tenacissima extract gavage. According to the pharmacokinetic parameters, tenacissoside B, I, H and G are the long-acting and primary bioactive constituents in M. tenacissima extract, with long mean residence times and high concentrations. Our findings shed light on the absorption mechanism and provide significant information for the clinical application of M. tenacissima.

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“…The derivatives of Tenacigenin B can reverse P‐glycoprotein‐mediated multidrug resistance in HepG2/Dox cells (Hu et al , ) and have cytotoxic activity against cancer cell lines (Luo et al , ). Tenacissoside H and Tenacissoside I are detected at relatively high levels in M. tenacissima , and Tenacissoside H serves as the marker compound for quality control (The State Commission of Chinese Pharmacopoeia, ; Wang et al , ) . Tenacissoside H and Tenacissoside I possess an identical oligosaccharide chain consisting of two sugar units at C‐3 of their aglycone.…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling of tenacigenin B, tenacissoside H and tenacissoside I using UHPLC‐ESI‐Orbitrap MS/MS

Zhao

Han

Ren

et al. 2016

Biomedical Chromatography

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Marsdenia tenacissima, which is widely used as an anticancer herb in traditional Chinese medicine, has been shown to possess anticancer activity. However, its metabolic profile is poorly investigated. Tenacigenin B is the major steroidal skeleton of C-21 steroids in M. tenacissima. Tenacissoside H and Tenacissoside I are detected at relatively high levels in M. tenacissima. Therefore, we studied their metabolic characteristics in human liver microsomes by ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry. Fourteen metabolites were tentatively identified by accurate mass measurement and MS/MS fragmentation behavior. It was found that hydroxylation reactions were the major metabolic pathway of Tenacissoside H and Tenacissoside I in human liver microsomes, whereas the metabolic pathway of Tenacigenin B involved dehydrogenation reactions. This is the first time that the metabolic profile of C-21 steroids from M. tenacissima has been explored in human liver microsomes, which is of great significance for subsequent pharmacokinetic and interaction research. Biotransformation in vivo or in vitro may influence the structure of a compound and change its activity. Identification of their fragmentation behaviors and metabolites provides valuable and new information for further understanding the anti-tumor activity of M. tenacissima. Copyright © 2016 John Wiley & Sons, Ltd.

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Simultaneous quantification of two steroidal glycosides after oral gavage of Marsdenia tenacissima extract in rats using a LC‐MS/MS method

Cited by 5 publications

References 16 publications

The Antitumor Activities of Marsdenia tenacissima

The Antitumor Activities of Marsdenia tenacissima

Simultaneous determination of eight bioactive components in Marsdenia tenacissima extract in rat plasma by LC–MS/MS and its application in a pharmacokinetic study

Metabolic profiling of tenacigenin B, tenacissoside H and tenacissoside I using UHPLC‐ESI‐Orbitrap MS/MS

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