Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry

Abstract

A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), repeatability (RSD < 4.36%), intra- and interday precisions (RSD < 5.07%) with good accuracies (97.76-105.08%) and good recovery (95.0-107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30-17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. The developed method could be used as a rapid, sensitive, and reliable approach for assessment of the quality of processed Fuzi and related decoction.

Serum metabolomics reveals betaine and phosphatidylcholine as potential biomarkers for the toxic responses of processed Aconitum carmichaelii Debx

“…4 In a recent quantitative analysis of processed Fuzi decoctions, including BFP, it was found that the contents of aconitine and mesaconitine were largely reduced by hydrolysis to monoester alkaloids during the processing, leading to a corresponding reduction in toxicity. 40 However, it now appears that at certain high doses of BFP, abundant amounts of the non-hydrolyzed toxic diester diterpene alkaloids still possess a certain degree of systemic toxicity, compensating for the NO-inhibitory potential of the cardioprotective alkaloid, higenamine.…”

Higenamine in Plants as a Source of Unintentional Doping

“…Since then, higenamine has been detected in the decoctions Baifupian and Heishunpian in similar amounts ranging from 2.31 to 3.18 μg/g. [ 70 ]. Nowadays, there are many processed products of Aconitum .…”

The Effects of Vinegar Processing on the Changes in the Physical Properties of Frankincense Related to the Absorption of the Main Boswellic Acids

“…Each concentration was analyzed in triplicate, and the calibration curves were established by plotting the peak areas versus the concentrations of each solution. The LOD and LLOQ were measured at signal-to-noise ratios (S/N) of 3 and 10, respectively, as per criteria from [50].…”

“…The equilibrium solubilities of the samples were determined by the shake-flask method [50]. Briefly, the accurately weighed solid sample was carefully added to 10 mL of the aqueous buffer in a glass vial and stirred at (37 ± 1) °C for 24 h. The aliquots of supernatant were taken out and diluted with solvent if necessary, and the concentration of sample in each aliquot was measured by UHPLC-TQ-MS.…”