Simultaneous quantitation of volatile compounds in citrus beverage through stir bar sorptive extraction coupled with thermal desorption-programmed temperature vaporization

Cheong, Mun Wai; Lee, Justin Yong Kiang; Liu, Shao-Quan; Zhou, Weibiao; Nie, Yunyun; Kleine-Benne, Eike; Curran, Philip; Yu, Bin

doi:10.1016/j.talanta.2012.12.034

Cited by 8 publications

(3 citation statements)

References 37 publications

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“…A number of studies has demonstrated successful application of the cryofocusing-GCmass spectrometry (MS) system to trace gas analysis, such as volatile organic compounds (VOCs) in the air and the exhaled breath. [1][2][3][4][5][6][7][8][9] In the typical cryofocusing-GC-MS system, the cryofocusing typically takes place at the inlet of the GC column. The sample enrichment occurs by quench-condensation at sub-ambient temperature.…”

Section: Introductionmentioning

confidence: 99%

Temperature Programmed Desorption of Quench-condensed Krypton and Acetone in Air; Selective Concentration of Ultra-trace Gas Components

Suzuki

Sakaguchi

2016

ANAL. SCI.

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Selective concentration of ultra-trace components in air-like gases has an important application in analyzing volatile organic compounds in the gas. In the present study, we examined quench-condensation of the sample gas on a ZnO substrate below 50 K followed by temperature programmed desorption (TPD) (low temperature TPD) as a selective gas concentration technique. We studied two specific gases in the normal air; krypton as an inert gas and acetone as a reactive gas. We evaluated the relationship between the operating condition of low temperature TPD and the lowest detection limit. In the case of krypton, we observed the selective concentration by exposing at 6 K followed by thermal desorption at about 60 K. On the other hand, no selectivity appeared for acetone although trace acetone was successfully concentrated. This is likely due to the solvent effect by a major component in the air, which is suggested to be water. We suggest that pre-condensation to remove the water component may improve the selectivity in the trace acetone analysis by low temperature TPD.

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Section: Introductionmentioning

confidence: 99%

Temperature Programmed Desorption of Quench-condensed Krypton and Acetone in Air; Selective Concentration of Ultra-trace Gas Components

Suzuki

Sakaguchi

2016

ANAL. SCI.

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“…[11][12][13][14][15][16][17][18][19] In the typical cryofocusing-GC-MS system, the cryofocusing typically takes place at the inlet of the GC column. The sample enrichment occurs by quenchcondensation at sub-ambient temperatures.…”

Section: Introductionmentioning

confidence: 99%

Selective Concentration of Ultra-trace Acetone in the Air by Cryogenic Temperature Programmed Desorption (cryo-TPD)

Suzuki

Sakaguchi

2016

ANAL. SCI.

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A gas analytical technique with compact size, low cost, sufficient sensitivity, and excellent reproducibility is required in many fields including exhaled breath analysis for medical monitoring. In the present study, we examined selective acetone concentration by quench condensation at cryogenic temperature followed by temperature programmed desorption (cryogenic temperature programmed desorption (cryo-TPD)) for possible applications to breath analysis for medical monitoring. The essence of cryo-TPD is rough mass selection by thermal desorption followed by quantification of certain species using mass spectrometry. The performance of cryo-TPD was investigated in the acetone concentration range below 1 × 10 -6 volume fraction (1 ppmv). It was found that acetone is selectively quench-condensed on a tungsten substrate at 50 K without the major components of air, such as N2 and O2. The concentrated acetone gas was obtained by the following thermal desorption at around 151 K. Under conditions of condensation for 1 min and pressure of 1 × 10 -2 Pa, the lowest limit of detection reached well below 10 × 10 -9 volume fraction (10 ppbv). The relationship between the cetone intensity of cryo-TPD and the acetone concentration in the gas was almost linear in the ppbv range. The separation of acetone and propanal using the fragmentation pattern, which have almost the identical molecular mass, was also demonstrated in the present study.

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“…Flavour compounds have a wide range of applications in food, beverage, cosmetic and pharmaceutical fields (Noguerol-Pato et al 2012 ; Cheong et al 2013 ; Descours et al 2013 ; Madruga et al 2013 ; Zamzuri and Abd-Aziz, 2013 ). In the global consumer-driven market, one of the challenges for manufacturers is to create products faster and cheaper while continuing to offer consumers a recognizable ‘sensory experience’.…”

Section: Introductionmentioning

confidence: 99%

Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA:ethanol O-acyltransferase Eht1 or Eeb1

Zhuang

Powell

et al. 2015

SpringerPlus

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Medium-chain volatile flavour esters are important molecules since they have extensive applications in food, fragrance, cosmetic, paint and coating industries, which determine different characteristics of aroma or taste in commercial products. Biosynthesis of these compounds by alcoholysis is catalyzed by acyl-CoA:ethanol O-acyltransferases Eht1 or Eeb1 in Saccharomyces cerevisiae. In this study, these two yeast enzymes were selected to explore their preparations as the form of whole cell biocatalysts for the production of volatile flavour esters. Here, the novel whole cell biocatalysts Pichia pastoris yeasts with functional extracellular expression of Eht1 or Eeb1 were constructed. Flavour production was established through an integrated process with coupled enzyme formation and ester biosynthesis in the recombinant yeasts in one pot, leading to the formation of volatile C6–C14 methyl and ethyl esters from wort medium. Interestingly, there is no significant difference between P. pastoris-EHT1 and P. pastoris-EEB1 in substrate preference during flavour biosynthesis, indicating a similar role of Eht1 and Eeb1 in P. pastoris cells, in contradiction with previous findings in S. cerevisiae to some extent. Consequently the study not only provides a greater understanding of these two enzymes in a heterogeneous host, but also demonstrated the positive effect of the recombinant Eht1 and Eeb1 in ester formation by P. pastoris live cells, potentially paving the way towards achieving efficient production of volatile flavour by an integrated biocatalytic system composed of recombinant enzyme production and flavour biosynthesis.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-015-1195-0) contains supplementary material, which is available to authorized users.

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Simultaneous quantitation of volatile compounds in citrus beverage through stir bar sorptive extraction coupled with thermal desorption-programmed temperature vaporization

Cited by 8 publications

References 37 publications

Temperature Programmed Desorption of Quench-condensed Krypton and Acetone in Air; Selective Concentration of Ultra-trace Gas Components

Temperature Programmed Desorption of Quench-condensed Krypton and Acetone in Air; Selective Concentration of Ultra-trace Gas Components

Selective Concentration of Ultra-trace Acetone in the Air by Cryogenic Temperature Programmed Desorption (cryo-TPD)

Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA:ethanol O-acyltransferase Eht1 or Eeb1

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