Simultaneous quantitative analysis of salivary cortisol and cortisone in Korean adults using LC-MS/MS

Abstract

The levels of salivary cortisol and cortisone in Korean adults were measured for the first time using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The salivary cortisol and cortisone were separated within 10 min. The regression coefficients (r) of the calibration curves were greater than 0.999 for the two steroids. The limits of quantitation (LOQ) were 0.2 ng/ml for cortisol and 1 ng/ml for cortisone. The intra-day precisions of the assay were ＜3.9% and 8.6% for cortisol and cortisone respectively, and the inter-day precisions were ＜1.9% and 4.3% for cortisol and cortisone, respectively. The salivary cortisone concentrations were approximately 4-9 times higher than those of salivary cortisol during the daytime. Diurnal rhythms, during which the cortisol and cortisone concentrations were higher in the morning than in the afternoon, were also observed. The present assay may be useful for the diagnosis of several adrenal dysfunctions in clinical biochemistry. [BMB reports 2010; 43(7): 506-511]

11β-hydroxysteroid dehydrogenase type-II activity is affected by grapefruit juice and intense muscular work

“…Our baseline CON values for the E:F ratio were similar to those previously reported by others (6). The short term GFJ treatment in the present study lowered enzyme activity by 44% (Figure 3), which compares favorably to the ~40% inhibition estimated from the urinary-E/F ratio obtained in a pilot study (10) and to the ~60% obtained in a patient presenting with edema and hypokalemia associated with the habitual oral intake of 1 liter/day of GFJ (5).…”

“…This enzyme oxidizes cortisol (F) into inactive cortisone (E), thereby rendering the mineralocorticoid receptor-hormone complex inactive (1). The enzymatic activity of 11β-HSD2 can be estimated using the ratios of these hormones in urine (2)(3)(4)(5) and saliva (Sal) fluids (4,6,7). Subtle deficiencies in 11β-HSD2 activity have been reported in subsets of hypertensives and normotensives (8,9), whereas more severe hypertension and hypokalemia are observed in cases of substantial or complete loss of 11β-HSD2 activity (1,9).…”

Measurement of Late-Night Salivary Cortisol and Cortisone by LC-MS/MS to Assess Preanalytical Sample Contamination with Topical Hydrocortisone

“…Using these creams or ointments can cause preanalytical contamination of the saliva sample that cannot be distinguished from endogenous cortisol as measured by either immunoassay or liquid chromatography-tandem mass spectrometry (LC-MS/MS). With endogenous cortisol production, the cortisone concentration in the saliva is usually higher than the cortisol concentration, and typically the cortisol-to-cortisone ratio is Ͻ1, owing to the transfer of cortisone from the plasma and the conversion of cortisol to cortisone by 11-␤-hydroxysteroid dehydrogenase type 2 (11-␤-HSD2) in the salivary gland (2,3 ). Contamination of the saliva with topical or oral hydrocortisone (cortisol) during sample collection would not be expected to increase salivary cortisone because there is no 11-␤-HSD2 activity in collected saliva (3 ).…”

Simultaneous measurement of endogenous cortisol, cortisone, dehydroepiandrosterone, and dehydroepiandrosterone sulfate in nails by use of UPLC–MS–MS

“…LC-MS/MS technology provides the opportunity to accurately determine hormone concentrations even in small amounts of sample. It has been shown previously that adrenal hormones can be assessed in biological fluids via LC-MS/MS [20][21][22][23].…”