Simultaneous Quantitative Detection of Relevant Biomarkers in Breast Cancer by Quantitative Real-Time PCR

Labuhn, Martin; Vuaroqueaux, Vincent; Fina, Frédéric; Schaller, André; Nanni-Metellus, Isabelle; Küng, Willy; Eppenberger‐Castori, Serenella; Martin, Pièrre‐Marie; Eppenberger, Urs

doi:10.1177/172460080602100105

Cited by 16 publications

(6 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The comparison between immunohistochemical and mRNA expression analysis further confirmed discrepancies previously described by other studies [25][26][27]. A higher similarity and consistency between protein levels and gene expression as determined by qRT-PCR were observed.…”

Section: Discussionsupporting

confidence: 90%

Evaluation of estrogen receptor α and β and progesterone receptor expression and correlation with clinicopathologic factors and proliferative marker Ki-67 in breast cancers

et al. 2008

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 90%

Evaluation of estrogen receptor α and β and progesterone receptor expression and correlation with clinicopathologic factors and proliferative marker Ki-67 in breast cancers

et al. 2008

View full text Add to dashboard Cite

“…A study has compared realtime-qPCR results for the assessment of mRNA levels of ERa, PgR, and the members of the human epidermal growth factor receptor family, HER1, HER2, HER3 and HER4 (Labuhn et al 2006 ). The results were obtained in two independent laboratories using two different methods, SYBR Green I and TaqMan probes, and different primers.…”

Section: Real Time Qualitative Pcr (Real Time-qpcr) Assays)mentioning

confidence: 99%

Personalized Therapy of Cancer

Jain

2015

Textbook of Personalized Medicine

View full text Add to dashboard Cite

“…A number of studies have used quantitative real-time PCR for the assessment of HER-2 status (Bieche et al 1999; O’Malley et al 2001; Kim et al 2002; Mrhalova et al 2003; Königshoff et al 2003; Ginestier et al 2004; Schlemmer et al 2004; Suo et al 2004; Gjerdrum et al 2004; Vanden Bempt et al 2005; Esteva et al 2005; Benöhr et al 2005; Bossard et al 2005; Tse et al 2005; Vinatzer et al 2005; Kulka et al 2006; Ntoulia et al 2006; Labuhn et al 2006). These studies investigated the extent of concordance of IHC, FISH (or CISH), quantitative PCR, and in some cases quantitative RT-PCR.…”

Section: Her-2 and Breast Cancermentioning

confidence: 99%

The epidermal growth factor receptor family in breast cancer

Koutras

Evans²

2008

OTT

View full text Add to dashboard Cite

The human epidermal growth factor receptor (HER) family comprises four homologous members: EGFR, HER-2, HER-3, and HER-4. The activation of these receptors triggers a complex series of signal transduction pathways which affect pivotal tumorigenic processes. The deregulation of HER signaling is seen in several human malignancies. HER-2 is now recognized as a key oncogene in breast cancer pathogenesis. Assessment of HER-2 status is of central importance in the prognosis of breast cancer patients. In the light of clinical data suggesting that HER-2 can also be useful as a predictive marker both for trastuzumab and chemotherapy, standardized determination of the HER-2 status in tumors has become more important. Moreover, current data provide evidence for the significance of HER-3 and HER-4 alterations in breast carcinogenesis. Because of the complex interactions among the HER receptors, it is likely that the effect on cell proliferation and tumor growth depends on receptor trans-signaling and thus, the evaluation of the combined expression pattern of all family members is of particular interest. This review presents the current evidence highlighting the role of the family as a whole panel and an update on the role of HER-3 and HER-4 receptors in breast cancer. Moreover, we provide updated data regarding the prognostic value of HER family members giving emphasis to novel methods for the determination of their status, such as real-time polymerase chain reaction. In addition, we review recent therapeutic approaches aimed at targeting the HER family in breast cancer patients.

show abstract

Simultaneous Quantitative Detection of Relevant Biomarkers in Breast Cancer by Quantitative Real-Time PCR

Cited by 16 publications

References 39 publications

Evaluation of estrogen receptor α and β and progesterone receptor expression and correlation with clinicopathologic factors and proliferative marker Ki-67 in breast cancers

Evaluation of estrogen receptor α and β and progesterone receptor expression and correlation with clinicopathologic factors and proliferative marker Ki-67 in breast cancers

Personalized Therapy of Cancer

The epidermal growth factor receptor family in breast cancer

Contact Info

Product

Resources

About