Simultaneous quantitative determination of NG,NG-dimethyl-l-arginine or asymmetric dimethylarginine and related pathway's metabolites in biological fluids by ultrahigh-performance liquid chromatography/electrospray ionization-tandem mass spectrometry

Gangi, Iole Maria Di; Chiandetti, Lino; Gucciardi, Antonina; Moret, V.; Naturale, Mauro; Giordano, Giuseppe

doi:10.1016/j.aca.2010.08.011

Cited by 56 publications

(37 citation statements)

References 53 publications

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“…Similarly high R.S.D. (s) were reported by El Khoury et al [12] or even higher values by Di Gangi et al and Shin et al [11,13]. The improvement regarding the precision in the current method was attributable to the introduction of the stable isotope labeled I.S.…”

Section: Precision and Accuracysupporting

confidence: 80%

See 1 more Smart Citation

Quantification of l-arginine, asymmetric dimethylarginine and symmetric dimethylarginine in human plasma: A step improvement in precision by stable isotope dilution mass spectrometry

Martens‐Lobenhoffer

Bode‐Böger

2012

Journal of Chromatography B

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Section: Precision and Accuracysupporting

confidence: 80%

“…(s) for every analyte to be quantified [8]. Unfortunately, even in recent publications [11][12][13], no dedicated stable isotope labeled I.S. for SDMA was used.…”

Section: Introductionmentioning

confidence: 98%

Quantification of l-arginine, asymmetric dimethylarginine and symmetric dimethylarginine in human plasma: A step improvement in precision by stable isotope dilution mass spectrometry

Martens‐Lobenhoffer

Bode‐Böger

2012

Journal of Chromatography B

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“…To these samples, 10% sulfosalicylic acid was added to precipitate the protein, and 1 M norvaline was used as an internal standard. Intracellular amino acids were then measured with an UltraPerformance LC system (Waters) (33).…”

Section: Gdh Enzyme Assay Insulin Secretion and Cytosolic Calcium Mmentioning

confidence: 99%

Green Tea Polyphenols Control Dysregulated Glutamate Dehydrogenase in Transgenic Mice by Hijacking the ADP Activation Site

Chen

et al. 2011

Journal of Biological Chemistry

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Glutamate dehydrogenase (GDH) catalyzes the oxidative deamination of L-glutamate and, in animals, is extensively regulated by a number of metabolites. Gain of function mutations in GDH that abrogate GTP inhibition cause the hyperinsulinism/hyperammonemia syndrome (HHS), resulting in increased pancreatic ␤-cell responsiveness to leucine and susceptibility to hypoglycemia following high protein meals. We have previously shown that two of the polyphenols from green tea (epigallocatechin gallate (EGCG) and epicatechin gallate (ECG)) inhibit GDH in vitro and that EGCG blocks GDH-mediated insulin secretion in wild type rat islets. Using structural and site-directed mutagenesis studies, we demonstrate that ECG binds to the same site as the allosteric regulator, ADP. Perifusion assays using pancreatic islets from transgenic mice expressing a human HHS form of GDH demonstrate that the hyperresponse to glutamine caused by dysregulated GDH is blocked by the addition of EGCG. As observed in HHS patients, these transgenic mice are hypersensitive to amino acid feeding, and this is abrogated by oral administration of EGCG prior to challenge. Finally, the low basal blood glucose level in the HHS mouse model is improved upon chronic administration of EGCG. These results suggest that this common natural product or some derivative thereof may prove useful in controlling this genetic disorder. Of broader clinical implication is that other groups have shown that restriction of glutamine catabolism via these GDH inhibitors can be useful in treating various tumors. This HHS transgenic mouse model offers a highly useful means to test these agents in vivo.

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“…Unfortunately, these HPLC methods are very time consuming. Since 2000, ADMA and SDMA have also been detected by liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/tandem mass spectrometry (LC/ MS/MS) [16][17][18][19][20][21]. Although detection of a selective product ion with MS/MS fragmentation can be difficult, Bishop, et al and Zotti, et al have been reported that the selective product ions for ADMA and SDMA are at m/z 203→46 and 203→172, respectively [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, though these methods are more sensitive, ADMA and SDMA have the different pattern of dissociation using various MS system. Di Gangi, et al have developed a reliable method using ultra per-formance LC/MS /MS that is simple and has a short analytical time [21]. This method is sensitive, however preparation for derivatization is time consuming, and ADMA and SDMA have not been separated successfully.…”

Section: Introductionmentioning

confidence: 99%

Determination of Asymmetric Dimethylarginine and Symmetric Dimethylarginine in Biological Samples of Mice Using LC/MS/MS

Saigusa¹,

Takahashi²,

Kanemitsu³

et al. 2011

AJAC

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Herein, we present a novel method of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) determination within biological samples using protein precipitation and LC/MS/MS. Chromatographic separation of ADMA and SDMA was successfully performed using a silica column with optimized elution, or mobile phase, of 10 mM ammonium acetate buffer H 2 O/methanol/acetonitrile (20/35/45, v/v) at pH 4. The calibration ranges were 0.50 -50.0 µg·mL -1 , and good linearities were obtained for all compounds ( r > 0.99). The intra-and inter-assay accuracies with recoveries and precisions at three concentration levels (i.e. 1.00, 5.00 and 25.0 µg·mL -1 ) were better than 86.9% and 7.36%, respectively. The analytical performance of the method was evaluated by determination of compounds in plasma, urine and tissues from male BALBc/J mice. For the first time, we were able to characterize the distribution of ADMA, SDMA and AD-MA/SDMA in plasma, urine, brain, heart, kidneys, liver, lungs, pancreas and spleen. Additionally, we demonstrated that the ADMA/SDMA ratio in the brain was approximately 10-fold lower than all the other biological samples. Only 10 µL of plasma, 1 µL of urine and about 25 mg of tissues were required. These results suggest that the developed methodology was useful in ADMA and SDMA determination within biological samples.

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Simultaneous quantitative determination of NG,NG-dimethyl-l-arginine or asymmetric dimethylarginine and related pathway's metabolites in biological fluids by ultrahigh-performance liquid chromatography/electrospray ionization-tandem mass spectrometry

Cited by 56 publications

References 53 publications

Quantification of l-arginine, asymmetric dimethylarginine and symmetric dimethylarginine in human plasma: A step improvement in precision by stable isotope dilution mass spectrometry

Quantification of l-arginine, asymmetric dimethylarginine and symmetric dimethylarginine in human plasma: A step improvement in precision by stable isotope dilution mass spectrometry

Green Tea Polyphenols Control Dysregulated Glutamate Dehydrogenase in Transgenic Mice by Hijacking the ADP Activation Site

Determination of Asymmetric Dimethylarginine and Symmetric Dimethylarginine in Biological Samples of Mice Using LC/MS/MS

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