Simultaneous quantitative determination of residues of abamectin, ivermectin, albendazole and its three metabolites in beef and chicken by HPLC-PDA

Shi, Xinrui; Guo, Yawen; Guan, Fanxun; Gao, Pengfei; Tang, Yayun; Zhu, Yali; Xie, Kaizhou; Chen, Hailan

doi:10.1016/j.foodchem.2023.136168

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…Livestock and poultry meat contain endogenous substances, such as proteins and lipids, that can interfere with AAS residue detection [ 13 ]. Therefore, the selection of a suitable purification method is one effective means of ensuring the accuracy of quantification.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Simultaneous Determination of Anabolic Andro-Genic Steroids in Livestock and Poultry Meat Using One-Step Solid-Phase Extraction Coupled with UHPLC–MS/MS

Wang,

Yan,

Wang

et al. 2023

Molecules

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Anabolic androgenic steroids (AASs) are usually illegally added to animal feed because they can significantly promote animal growth and increase carcasses’ leanness, which threatens the safety of animal-derived foods and indirectly hazards human health. This study aimed to establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) method for the simultaneous detection of twelve AAS residues in livestock and poultry meat. The homogenized samples were extracted with acetonitrile containing 1% acetic acid (v/v) and purified using the one-step extraction column. After concentration using nitrogen, the residues were redissolved in acetonitrile and then quantified with an external standard method using UHPLC–MS/MS. The results showed that the above-mentioned method had a satisfactory linear correlation (R2 ≥ 0.9903) with a concentration range of 1–100 μg/L, and the limits of detection (LODs) and quantification (LOQs) were 0.03–0.33 μg/kg and 0.09–0.90 μg/kg, respectively. With the intraday and interday precision less than 15%, the average recoveries of pork, beef, lamb, and chicken, at different spiked levels, ranged from 68.3 to 93.3%, 68.0 to 99.4%, 71.6 to 109.8%, and 70.5 to 97.7%, respectively. Overall, the established method is validated, precise, and capable of the high-throughput determination of the residues of twelve AASs in livestock and poultry meat.

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Section: Introductionmentioning

confidence: 99%

Rapid and Simultaneous Determination of Anabolic Andro-Genic Steroids in Livestock and Poultry Meat Using One-Step Solid-Phase Extraction Coupled with UHPLC–MS/MS

Wang,

Yan,

Wang

et al. 2023

Molecules

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A novel HPLC-PDA method for judging chicken meat infected with Salmonella Enteritidis by simultaneous determination of four key metabolite biomarkers

Xu,

Ding,

Chen

et al. 2024

Journal of Food Composition and Analysis

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Application of Elisa and HPLC-MS/MS Methods in Clinical Trials of Veterinary Drugs for the Determinaton of Ivermectin Content

Yanovych,

Zasadna,

Rydchuk

et al. 2024

SCIVP

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The article presents the results of the development and validation of screening method and chromatographic mass spectrometric method for the determining of ivermectin residual amounts in muscles and parenchymal tissues of pigs and calves, as well as in blood plasma, for clinical trials (for withdrawal period establishing and bioequivalence study of drugs based on ivermectin). EuroProxima Ivermectin test kits (Netherlands) were used to determine ivermectin residues in animal tissues. When carrying out the validation at the levels of ½ MRL, which is 15 and 30 μg/kg for muscle and parenchymal tissues, the LOD for the analyte was established at the levels of 2.7 and 4.1 μg/kg, respectively. To establish the percent of analyte’s recovery from the spiked samples, selected blank samples and samples, spiked with ivermectin solution at ½ MRL and MRL levels, were prepared and analyzed according to the technique provided by the test kit manufacturer. The percentage of analyte’s recovery for muscle tissues spiked at the level of 15.0, 30.0, 100.0 μg/kg was: 100.2, 100.4, 99.9, respectively. For liver tissues, spiked at the level of 50.0, 100.0, 500.0 μg/kg, it was 100.0, 99.95, 99.5, respectively. To establish the bioequivalence of veterinary drugs based on ivermectin, we have developed and validated high-performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS) method. The suggested method of sample preparation includes the liquid-liquid extraction of the analyte with ethyl acetate from an acidic medium and concentration by drying. Analysis of the prepared samples was performed by HPLC-MS/MS (analyte’s retention time 2.64 min, mass scanning parameters: precursor ion 892.8 m/z, product ions 307.2 and 569.4 m/z). Various key parameters were used to validate the chromatographic technique: limit of detection, limit of quantification, working and linear concentration ranges, accuracy, and precision. The results of the performed validation are presented in the article in tables and chromatograms, and conclusions about the fitness-for-purpose of the method are also made.

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Simultaneous quantitative determination of residues of abamectin, ivermectin, albendazole and its three metabolites in beef and chicken by HPLC-PDA

Cited by 3 publications

References 34 publications

Rapid and Simultaneous Determination of Anabolic Andro-Genic Steroids in Livestock and Poultry Meat Using One-Step Solid-Phase Extraction Coupled with UHPLC–MS/MS

Rapid and Simultaneous Determination of Anabolic Andro-Genic Steroids in Livestock and Poultry Meat Using One-Step Solid-Phase Extraction Coupled with UHPLC–MS/MS

A novel HPLC-PDA method for judging chicken meat infected with Salmonella Enteritidis by simultaneous determination of four key metabolite biomarkers

Application of Elisa and HPLC-MS/MS Methods in Clinical Trials of Veterinary Drugs for the Determinaton of Ivermectin Content

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