1993
DOI: 10.1002/jsfa.2740630109
|View full text |Cite
|
Sign up to set email alerts
|

Simultaneous, rapid, spectrophotometric determination of total starch, amylose and amylopectin

Abstract: A novel spectrophotometric method for the simultaneous measurement of total starch, amylose and amylopectin in lipid-free samples has been developed using a diode-array spectrophotometer. It involves measurement of the spectrum of the amylose/amylopectin-iodide complexes at six wavelengths and calculation of amylose and amylopectin concentrations using multi-component analysis and a spreadsheet computer program. This method appears to offer some advantages to previous 'iodine-blue colour' assays for the measur… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

2
114
0
5

Year Published

1999
1999
2023
2023

Publication Types

Select...
10

Relationship

0
10

Authors

Journals

citations
Cited by 179 publications
(121 citation statements)
references
References 9 publications
2
114
0
5
Order By: Relevance
“…0.05 mL were diluted to 5 mL with 4.85 mL of distilled water and 0.1 mL of reagent (I 2 /KI) and incubated for 10 min. The absorbance of samples was carried out at 580 and 720 nm (Jarvis and Walker, 1993).…”
Section: Samplingmentioning
confidence: 99%
“…0.05 mL were diluted to 5 mL with 4.85 mL of distilled water and 0.1 mL of reagent (I 2 /KI) and incubated for 10 min. The absorbance of samples was carried out at 580 and 720 nm (Jarvis and Walker, 1993).…”
Section: Samplingmentioning
confidence: 99%
“…Starch content was determined using the AOAC (1995) Polarimetric method. The apparent amylose content was estimated by the six point wave length spectrophotometric analysis by Javis and Walker (1993).…”
Section: Physicochemical Propertiesmentioning
confidence: 99%
“…Amylose contents of jackfruit seed starch was determined in triplicate using the spectrophotometric method described by Javis and Walker (1993). Standard potato amylose and amylopectin (Sigma Chemical) were used as references.…”
Section: Chemical Analysesmentioning
confidence: 99%