Simultaneous recording of the field-EPSP as well as the population spike in the CA1 region in freely moving rats by using a fixed “double”-recording electrode

Scherf, Thomas; Frey, Julietta U.; Frey, Sabine

doi:10.1016/j.jneumeth.2010.01.020

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…Electrical stimulation of the CA3 causes monosynaptic activation of ipsilateral CA1 pyramidal cells (through Schaffer collaterals) and contralateral CA1 pyramidal cells (through commissural fibers). 9,10 Placing the recording electrode in the stratum radiatum of the contralateral CA1 allows the direct measurement of postsynaptic activity of pyramidal CA1 cells, the principal cells in this region. The rising slope of the stimulusevoked fEPSP is an uncontaminated reflection of the postsynaptic current and is proportional to the amplitude of that postsynaptic current.…”

Section: Introductionmentioning

confidence: 99%

Postsynaptic and Spiking Activity of Pyramidal Cells, the Principal Neurons in the Rat Hippocampal CA1 Region, Does Not Control the Resultant BOLD Response: A Combined Electrophysiologic and fMRI Approach

Scherf

Angenstein

2015

J Cereb Blood Flow Metab

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The specific role of postsynaptic activity for the generation of a functional magnetic resonance imaging (fMRI) response was determined by a simultaneous measurement of generated field excitatory postsynaptic potentials (fEPSPs) and blood oxygen level-dependent (BOLD) response in the rat hippocampal CA1 region during electrical stimulation of the contralateral CA3 region. The stimulation electrode was placed either in the left CA3a/b or CA3c, causing the preferentially basal or apical dendrites of the pyramidal cells in the right CA1 to be activated. Consecutive stimulations with low-intensity stimulation trains (i.e., 16 pulses for 8 seconds) resulted in clear postsynaptic responses of CA1 pyramidal cells, but in no significant BOLD responses. In contrast, consecutive high-intensity stimulation trains resulted in stronger postsynaptic responses that came along with minor (during stimulation of the left CA3a/b) or substantial (during stimulation of the left CA3c) spiking activity of the CA1 pyramidal cells, and resulted in the generation of significant BOLD responses in the left and right hippocampus. Correlating the electrophysiologic parameters of CA1 pyramidal cell activity (fEPSP and spiking activity) with the resultant BOLD response revealed no positive correlation. Consequently, postsynaptic activity of pyramidal cells, the most abundant neurons in the CA1, is not directly linked to the measured BOLD response.

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Section: Introductionmentioning

confidence: 99%

Postsynaptic and Spiking Activity of Pyramidal Cells, the Principal Neurons in the Rat Hippocampal CA1 Region, Does Not Control the Resultant BOLD Response: A Combined Electrophysiologic and fMRI Approach

Scherf

Angenstein

2015

J Cereb Blood Flow Metab

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“…Alongside the aforementioned technical advantages, the scope of data that can be obtained from fEPSP recordings is exceptionally wide: In addition to subthreshold activity, suprathreshold activity can be probed when a population spike is recognized as part of the evoked activity waveform ( Scherf et al., 2010 ). Further, both short-term plasticity ( Figure 8 ) and long-term plasticity can be induced with the proper stimulation protocol.…”

Section: Before You Beginmentioning

confidence: 99%

Measuring synaptic transmission and plasticity with fEPSP recordings in behaving mice

et al. 2022

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Summary Spontaneous spiking activity depends on intrinsic excitability and synaptic input. Historically, synaptic activity has been mostly studied ex vivo . Here, we describe a versatile and robust protocol to record field excitatory postsynaptic potentials (fEPSPs) in behaving rodents. The protocol allows estimating the input–output relationship of a specific pathway, short-term and long-term plasticity, and their modulation by pharmacological or pharmacogenetic interventions and behavioral states. However, experimenters must be aware of the protocol’s specificity and interpret results with care. For complete details on the use and execution of this profile, please refer to Styr et al. (2019) .

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Electrophysiological properties of hippocampal–cortical neural networks, role in the processes of learning and memory in rats

Guo

2014

J Neural Transm

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The recording of hippocampal and cortical long-term potentiation (LTP) in rats in vivo is an appropriate and commonly used method to describe changes in cellular mechanisms underlying synaptic plasticity. Recently, we introduced a method for the simultaneous recording of LTP in bilateral CA1 regions and parietal association cortex (PtA), and observed differences between the Schaffer collateral-CA1 pathway (SC), Schaffer collateral/associational commissural pathway (SAC) and Schaffer collateral/associational commissural-cortex pathway (SACC). In this study, we found that (1) synaptic transmission of the SAC and SACC pathways depended on hippocampal commissural fibers [dorsal and ventral hippocampal commissural fibers, the medial septum (MS) and hippocampal CA3 commissural fibers], (2) nerve conduction velocity of the SACC pathway might be higher than that of the SAC pathway, (3) the input/output (I/O) curve of the SC pathway was shifted to the left side, compared to that of the SAC and SACC pathways, (4) all three pathways could induce stable LTP; however, LTP of the SAC and SACC pathways was much stronger than that of the SC pathway, (5) the degree of paired-pulse facilitation (PPF) was weaker in the SC pathway than that in the SAC and SACC pathways, (6) after cutting off the corpus callosum and commissural fibers, spatial learning and memory were impaired, and the ability to explore the novel environment and spontaneous locomotor activity were weakened. Taken together, our results suggested that hippocampal commissural fibers were very important for exchanging information between hemispheres, and basic differences in electrophysiological properties of hippocampal-cortical neural networks play a vital role in the processes of learning and memory.

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Simultaneous recording of the field-EPSP as well as the population spike in the CA1 region in freely moving rats by using a fixed “double”-recording electrode

Cited by 5 publications

References 28 publications

Postsynaptic and Spiking Activity of Pyramidal Cells, the Principal Neurons in the Rat Hippocampal CA1 Region, Does Not Control the Resultant BOLD Response: A Combined Electrophysiologic and fMRI Approach

Postsynaptic and Spiking Activity of Pyramidal Cells, the Principal Neurons in the Rat Hippocampal CA1 Region, Does Not Control the Resultant BOLD Response: A Combined Electrophysiologic and fMRI Approach

Measuring synaptic transmission and plasticity with fEPSP recordings in behaving mice

Electrophysiological properties of hippocampal–cortical neural networks, role in the processes of learning and memory in rats

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