In this study, the biological removal of nitrogen and phosphorous (BNR) was investigated by applying modified MLE-OSA technique. To conduct this study, three pilot plants scale were designed and established: 1) MLE similar to the current method used in Sari Wastewater Treatment Plant as control reactor 2) MLE-OSA 4 with 4-h hydrolic retention time in sludge holding tank 3) MLE-OSA 6 with 6-h hydrolic retention time in sludge holding tank. In this modified process for combining OSA technique with MLE system, two anaerobic/anoxic tanks were installed in the return sludge line with capacities of 70 and 107 l for MLE-OSA 4 and MLE-OSA 6, respectively. To set up the process, outlet sewage of the primary settlement tank of Sari Wastewater Treatment Plant was used. After a period of 45-60 days and reaching the steady state, the reactors were operated and the main, controllable parameters and laboratory experiments such as DO, ORP, Temperature, pH, COD, BOD 5 , MLSS, and nutrients (N&P) were precisely analyzed according to standard methods for examination of water and wastewater. The results showed that utilizing MLE-OSA system with 4 and 6 h hydraulic retention times decreased the ORP by around 109 ± 9 to 160 ± 25 mv and increased sludge retention time from 29 to 33 days. Moreover the percentages of phosphorus removal efficiency in MLE, MLE-