Simultaneous Retrodialysis by Drug for Cefuroxime Using Meropenem as an Internal Standard—A Microdialysis Validation Study

Hanberg, Pelle; Bue, Mats; Öbrink-Hansen, Kristina; Kabel, Jesper; Thomassen, Maja; Tøttrup, Mikkel; Søballé, Kjeld; Stilling, Maiken

doi:10.1016/j.xphs.2019.11.014

Cited by 17 publications

(16 citation statements)

References 23 publications

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“…Several calibration methods can be used to determine RR, 5,7 with the retrodialysis by drug method being the most frequently applied in antimicrobial pharmacokinetic studies. 5,19,20 Retrodialysis by drug calibration is based on single estimates determined prior to or after the sampling period.…”

Section: Discussionmentioning

confidence: 99%

“…5,10 This demonstrates, that it is crucial to conduct detailed evaluations in both in vitro and in vivo studies before using the internal standard in microdialysis studies. 5,7,10 Benzylpenicillin has previously been used as an internal calibrator for piperacillin in clinical studies due to similar molecule size and the fact, that both antimicrobials are ß-lactams, but to our knowledge, it has not been thoroughly evaluated. 11,12 Piperacillin is a broad-spectrum, low protein-binding and high solubility ßlactam antimicrobial often given in combination with the ß-lactamase inhibitor, tazobactam.…”

Section: 5mentioning

confidence: 99%

“…RR determination can be performed by various calibrations methods such as the no-net-flux method, the low-flow-rate method, the retrodialysis by drug method and the retrodialysis by drug with an internal standard method. 5,7 The retrodialysis by drug method and the retrodialysis by drug method with an internal standard, also known as the internal standard method, is the most frequently applied calibration method. While calibration with the retrodialysis by drug method is limited to one or a few calibration points prior to or after the sampling period, the internal standard method allows for continuous calibration during the experiment.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Benzylpenicillin as an Internal Standard for Measurement of Piperacillin Bone Concentrations Via Microdialysis

Knudsen

Bue

Pontoppidan

et al. 2021

Journal of Pharmaceutical Sciences

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Section: Discussionmentioning

confidence: 99%

Section: 5mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Benzylpenicillin as an Internal Standard for Measurement of Piperacillin Bone Concentrations Via Microdialysis

Knudsen

Bue

Pontoppidan

et al. 2021

Journal of Pharmaceutical Sciences

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“…The semipermeable membrane is continuously perfused which prevent equilibrium across the membrane [9][10][11][12][13]. The catheters are normally calibrated to compensate for this effect [14,15]. However, when changes in the concentration ratios and variation between interventions or compartments are of interest, as they are for comparing ischemic marker concentrations between tourniquet-exposed and non-tourniquet-exposed leg and for ratios between markers (i.e., lactate/pyruvate), this is not essential.…”

Section: Microdialysismentioning

confidence: 99%

Tourniquet‐induced ischemia and reperfusion in subcutaneous tissue, skeletal muscle, and calcaneal cancellous bone

Hanberg

Bue

Kabel

et al. 2021

APMIS

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This study aimed to evaluated ischemic metabolites in subcutaneous tissue, skeletal muscle, and calcaneal cancellous bone before, during, and after tourniquet application in a simultaneous paired comparison of tourniquet‐exposed and non‐tourniquet‐exposed legs. Ten patients scheduled for hallux valgus or hallux rigidus surgery were included. Microdialysis catheters were placed to simultaneously and continuously sample the metabolites glucose, lactate, pyruvate, and glycerol bilaterally for 12 h in subcutaneous tissue, skeletal muscle, and calcaneal cancellous bone. A tourniquet was applied on the leg planned for surgery (inflation time: 15 min, mean tourniquet duration time (range): 65 (58;77) min). During tourniquet inflation, a 2‐ to 3‐fold increase of the mean lactate/pyruvate ratio was found for all investigated tissues in the tourniquet‐exposed leg compared with the non‐tourniquet‐exposed leg. The lactate/pyruvate ratio recovery time after tourniquet release was within 30 min for skeletal muscle, 60 min for subcutaneous tissue, and 130 min for calcaneal cancellous bone. Only the tourniquet‐exposed skeletal muscles were found to be ischemic during tourniquet inflation, defined by a significant increase of the lactate/pyruvate ratio exceeding the ischemic cutoff level of 25; however, this level decreased below 25 immediately after tourniquet release. The glycerol ratio increased instantly after inflation in the tourniquet‐exposed leg in skeletal muscle and subcutaneous tissue, and recovered within 60 (skeletal muscle) and 130 min (subcutaneous tissue) after tourniquet release. These findings suggest that applying tourniquet for approximately 1 h results in limited tissue ischemia and cell damage in subcutaneous tissue, skeletal muscle, and calcaneal cancellous bone.

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“…Intraosseous vancomycin administration into the proximal tibia is an easy and feasible local vancomycin application. Intraosseous vancomycin has previously been employed as a perioperative antibiotic prophylaxis in total knee replacement and revision surgery and suggested as enhancing the effectiveness of the perioperative antibiotic prophylaxis (Young et al, 2014;Harper et al, 2020;Young et al, 2018;Chin et al, 2018). These studies assessed vancomycin tissue concentrations by means of tissue specimens, which, however, is limited by its invasiveness, static sampling points, determination of the total, and not free drug concentration.…”

Section: Introductionmentioning

confidence: 99%

Vancomycin bone and tissue concentrations following tibial intraosseous administration – evaluated in a porcine model

Kipp

Hanberg

Slater

et al. 2021

J. Bone Joint Infect.

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Abstract. Introduction. Systemic perioperative vancomycin may not provide sufficient prophylactic target-site concentrations in the prevention of prosthetic joint infections. Intraosseous vancomycin potentially provides high target-site concentrations. The objective of the present study was to evaluate the local bone and tissue concentrations following tibial intraosseous vancomycin administration in a porcine model. Methods. Eight pigs received 500 mg diluted vancomycin (50 mg/mL) through an intraosseous cannula into the proximal tibial cancellous bone. No tourniquet was applied. Microdialysis was applied for sampling of vancomycin concentrations in adjacent tibial cancellous bone, in cortical bone, in the intramedullary canal of the diaphysis, in the synovial fluid of the knee joint, and in the subcutaneous tissue. Plasma samples were obtained as a systemic reference. Samples were collected for 12 h. Results. High vancomycin concentrations were found in the tibial cancellous bone with a mean peak drug concentration of 1236 (range 28–5295) µg/mL, which remained high throughout the sampling period. The mean (standard deviation) peak drug concentration in plasma was 19 (2) µg/mL, which was obtained immediately after administration. Peak drug concentration, time to peak drug concentration, and area under the concentration–time curve were within the same range in the intramedullary canal, the synovial fluid of the knee, and the subcutaneous tissue. Conclusion. Tibial intraosseous administration of vancomycin provided high concentrations in tibial cancellous bone throughout a 12 h period but with an unpredictable and wide range of peak concentration. The systemic absorption was high and immediate, thus mirroring an intravenous administration. Low mean concentrations were found in all the remaining compartments.

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Simultaneous Retrodialysis by Drug for Cefuroxime Using Meropenem as an Internal Standard—A Microdialysis Validation Study

Cited by 17 publications

References 23 publications

Evaluation of Benzylpenicillin as an Internal Standard for Measurement of Piperacillin Bone Concentrations Via Microdialysis

Evaluation of Benzylpenicillin as an Internal Standard for Measurement of Piperacillin Bone Concentrations Via Microdialysis

Tourniquet‐induced ischemia and reperfusion in subcutaneous tissue, skeletal muscle, and calcaneal cancellous bone

Vancomycin bone and tissue concentrations following tibial intraosseous administration – evaluated in a porcine model

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