Simultaneous saccharification and fermentation of steam‐pretreated bagasse using <i>Saccharomyces cerevisiae</i> TMB3400 and <i>Pichia stipitis</i> CBS6054

Rudolf, Andreas; Baudel, Henrique Macedo; Zacchi, Guido; Hahn‐Hägerdal, Bärbel; Lidén, Gunnar

doi:10.1002/bit.21636

Cited by 113 publications

(60 citation statements)

References 37 publications

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“…Our study shows that the favorable growth condition for cell mass production is likely due to the mixed sugars, where glucose is converted more readily than xylose. Our results compare favorably with previous reports on fermentation of sugarcane bagasse hydrolysate (Rudolf et al 2008). In contrast, Bellido et al (2011) found that xylose was not utilized in 168 h of fermentation experiments using Scheffersomyces (Pichia) stipitis DSM3651 on filtered hydrolysate of steam exploded wheat straw using the whole slurry with acetate, HMF and furfural concentrations at 1.52, 0.05 and 0.14 g/l, respectively.…”

Section: Discussionsupporting

confidence: 91%

Design and Optimization of Ethanol Production from Bagasse Pith Hydrolysate by a Thermotolerant Yeast Kluyveromyces sp. IIPE453 Using Response Surface Methodology

Gikonyo¹

2015

Sugarcane as Biofuel Feedstock

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Sugarcane bagasse is a potential feedstock for cellulosic ethanol production, rich in both glucan and xylan. This stresses the importance of utilizing both C 6 and C 5 sugars for conversion into ethanol in order to improve the process economics. During processing of the hydrolysate degradation products such as acetate, 5-hydroxymethylfurfural (HMF) and furfural are formed, which are known to inhibit microbial growth at higher concentrations. In the current study, conversion of both glucose and xylose sugars into ethanol in wet exploded bagasse hydrolysates was investigated without detoxification using Scheffersomyces (Pichia) stipitis CBS6054, a native xylose utilizing yeast strain. The sugar utilization ratio and ethanol yield (Y p/s ) ranged from 88-100% and 0.33-0.41 ± 0.02 g/g, respectively, in all the hydrolysates tested. Hydrolysate after wet explosion at 185°C and 6 bar O 2 , composed of mixed sugars (glucose and xylose) and inhibitors such as acetate, HMF and furfural at concentrations of 3.2 ± 0.1, 0.4 and 0.5 g/l, respectively, exhibited highest cell growth rate of 0.079 g/l/h and an ethanol yield of 0.39 ± 0.02 g/g sugar converted. Scheffersomyces stipitis exhibited prolonged fermentation time on bagasse hydrolysate after wet explosion at 200°C and 6 bar O 2 where the inhibitors concentration was further increased. Nonetheless, ethanol was produced up to 18.7 ± 1.1 g/l resulting in a yield of 0.38 ± 0.02 g/g after 82 h of fermentation.

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Section: Discussionsupporting

confidence: 91%

Design and Optimization of Ethanol Production from Bagasse Pith Hydrolysate by a Thermotolerant Yeast Kluyveromyces sp. IIPE453 Using Response Surface Methodology

Gikonyo¹

2015

Sugarcane as Biofuel Feedstock

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“…Generally, for the purposes of testing the enzyme digestibility of pretreated lignocellulose or simultaneous saccharification and fermentation (SSF), the level of cellulase used is greater than 5 FPU per g of cellulose (Rudolf et al, 2008;Yang et al, 2006). However, in order to investigate the synergism between cellulase and the bacterial expansin in this study, 0.012-0.6 FPU of cellulase per g of cellulose were used.…”

Section: Effect Of the Amount Of Cellulase On Synergism In Cellulose mentioning

confidence: 99%

Functional characterization of a bacterial expansin from Bacillus subtilis for enhanced enzymatic hydrolysis of cellulose

Kim

Lee

Bang

et al. 2008

Biotech & Bioengineering

150

140

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Expansin is a plant protein family that induces plant cell wall-loosening and cellulose disruption without exerting cellulose-hydrolytic activity. Expansin-like proteins have also been found in other eukaryotes such as nematodes and fungi. While searching for an expansin produced by bacteria, we found that the BsEXLX1 protein from Bacillus subtilis had a structure that was similar to that of a beta-expansin produced by maize. Therefore, we cloned the BsEXLX1 gene and expressed it in Escherichia coli to evaluate its function. When incubated with filter paper as a cellulose substrate, the recombinant protein exhibited both cellulose-binding and cellulose-weakening activities, which are known functions of plant expansins. In addition, evaluation of the enzymatic hydrolysis of filter paper revealed that the recombinant protein also displayed a significant synergism when mixed with cellulase. By comparing the activity of a mixture of cellulase and the bacterial expansin to the additive activity of the individual proteins, the synergistic activity was found to be as high as 240% when filter paper was incubated with cellulase and BsEXLX1, which was 5.7-fold greater than the activity of cellulase alone. However, this synergistic effect was observed when only a low dosage of cellulase was used. This is the first study to characterize the function of an expansin produced by a non-eukaryotic source.

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“…In this study, ammonium hydroxide will be used to carry out detoxification [81] in order to avoid the environmental and operational issues associated with over-liming. The yield of ethanol on sugar and other fermentation parameters for the present study are presented in Table 5, which are conservatively assumed based on previous performances in literature [26,27]. To ensure this yield, fed-batch fermentation is adopted, since this mode of fermentation can be 1.5 Yeast loading 1.5-2 g/l dry weight [25,74] Conversion to Ethanol 82.5% of pentose, 90% of hexose Fermentation Time 140 hours [75] times more efficient than batch fermentation [82].…”

Section: Ethanol Coproduction From Hemicellulosementioning

confidence: 99%

Bioelectricity Versus Bioethanol From Sugarcane Bagasse: Is It Worth Being Flexible?

2015

Sugarcane as Biofuel Feedstock

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Background: The economics of producing only electricity from residues, which comprise of surplus bagasse and 50% post-harvest residues, at an existing sugar mill in South Africa was compared to the coproduction of ethanol from the hemicelluloses and electricity from the remaining solid fractions. Six different energy schemes were evaluated. They include: (1) exclusive electricity generation by combustion with high pressure steam cycles (CHPSC-EE), (2) biomass integrated gasification with combined cycles (BIGCC-EE), (3) coproduction of ethanol (using conventional distillation (CD)) and electricity (using BIGCC), (4) coproduction of ethanol (using CD) and electricity (using CHPSC), (5) coproduction of ethanol (using vacuum distillation (VD)) and electricity (using BIGCC), and (6) coproduction of ethanol (using VD) and electricity (using CHPSC). The pricing strategies in the economic analysis considered an upper and lower premium for electricity, on the standard price of the South African Energy Provider Eskom' of 31 and 103% respectively and ethanol prices were projected from two sets of historical prices.

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Simultaneous saccharification and fermentation of steam‐pretreated bagasse using Saccharomyces cerevisiae TMB3400 and Pichia stipitis CBS6054

Cited by 113 publications

References 37 publications

Design and Optimization of Ethanol Production from Bagasse Pith Hydrolysate by a Thermotolerant Yeast Kluyveromyces sp. IIPE453 Using Response Surface Methodology

Design and Optimization of Ethanol Production from Bagasse Pith Hydrolysate by a Thermotolerant Yeast Kluyveromyces sp. IIPE453 Using Response Surface Methodology

Functional characterization of a bacterial expansin from Bacillus subtilis for enhanced enzymatic hydrolysis of cellulose

Bioelectricity Versus Bioethanol From Sugarcane Bagasse: Is It Worth Being Flexible?

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