Simultaneous separation and determination of four active ingredients in Picria fel‐terrae Lour. and its preparations by micellar electrokinetic chromatography

Abstract

Introduction: Picfeltarraenins IA, IB and IV and acteoside are the four bioactive ingredients of Picria fel-terrae Lour. Their pharmacological effects include central inhibitory, cardiovascular, anti-inflammatory, anti-pyretic, analgesic, anti-bacterial, antioxidative and anti-tumor effects.Objective: We aimed to develop an efficient micellar electrokinetic chromatography (MEKC) method modified with mixed organic solvents for the simultaneous separation and determination of the four components in Picriae Herba and its formulations.Methods: Method optimization was carried out by investigating influences of significant factors on the separation, and this method was successfully applied for the determination of the four components in Picriae Herba and its formulations.Results: The optimal running buffer was composed of 20 mM sodium tetraborate, 40 mM sodium cholate, 10% (v/v) methanol and 10% (v/v) isopropanol (pH 9.76).The separation voltage was 18 kV, the temperature was 25 C and the detection wavelength was 266 nm. Under the optimal separation conditions, the baseline separation of four components was achieved in less than 14 min. The correlation coefficients of the calibration curves were 0.9984-0.9995 for the analytes. The intraday and interday precision ranged from 1.5% to 2.5% and from 1.4% to 5.0%, respectively. Recoveries of analytes varied from 96.6% to 104.1%. Conclusion:The method was proved suitable for the determination of four components in Picriae Herba and its formulations. Good performance was obtained under optimal conditions, and the method provides an effective tool for the quality control of Picriae Herba and its formulations.

Simultaneous separation and determination of seven biphenyl cyclooctene lignans in Schisandra chinensis and its preparations by micellar electrokinetic chromatography with dual organic solvent system

“…As a common anionic surfactant, SDS can improve the separation efficiency with increasing concentration, but a too high concentration will lead to an increase in electrical conductivity, resulting in a decrease in separation efficiency. In MEKC, it can adjust the component capacity factor as in chromatography, change the viscosity and zeta potential of the solution, and thus improve the separation selectivity of MEKC 34 . The addition of SDS improved the separation degree of the seven analytes, but the peaks of Gomisin H, D, E, and N could not be completely separated, and the peaks were poor in shape.…”

“…In MEKC, it can adjust the component capacity factor as in chromatography, change the viscosity and zeta potential of the solution, and thus improve the separation selectivity of MEKC. 34 The addition of SDS improved the separation degree of the seven analytes, but the peaks of Gomisin H, D, E, and N could not be completely separated, and the peaks were poor in shape. Therefore, we considered adding organic modifiers.…”

Simultaneous Separation and Determination of Nine Active Ingredients in Sanyetangzhiqing by Cyclodextrin-Modified Micellar Electrokinetic Capillary Electrophoresis-Diode Array Detector

“…Using micellar as pseudostationary phases, analytes are separated by differential partitioning between the micelle phase and the aqueous phase in MEKC [ 21 ]. On account of the characteristics of surfactants, both ionic compounds and neutral compounds could be separated by MEKC, which is especially suitable for the analysis of complex compounds extracted from TCMs [ 24 ]. Thus, in this paper, the MEKC mode of CE was selected for detection and separation of the components in SYTZQ.…”

Simultaneous Separation and Determination of Nine Active Ingredients in Sanyetangzhiqing by Cyclodextrin Modified Micellar Electrokinetic Capillary Electrophoresis-Diode Array Detector

“…Using micellar as pseudostationary phases, analytes are separated by diferential partitioning between the micelle phase and the aqueous phase in MEKC [21]. On account of the characteristics of surfactants, both ionic compounds and neutral compounds could be separated by MEKC, which is especially suitable for the analysis of complex compounds extracted from TCMs [24]. Tus, in this paper, the MEKC mode of CE was selected for detection and separation of the components in SYTZQ.…”