Simultaneous Solid Phase Extraction, Derivatization, and Gas Chromatographic Mass Spectrometric Quantification of Thromboxane and Prostacyclin Metabolites, Prostaglandins, and Isoprostanes in Urine

Wübert, Joachim; Reder, Elke; Kaser, Andrea; Weber, Peter; Lorenz, Reinhard

doi:10.1021/ac9611430

Cited by 27 publications

(7 citation statements)

References 22 publications

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“…Before analysis with gas chromatography (GC), all the samples were dried under a nitrogen stream and derivatized to their trimethylsilylethers. For this, samples were generated for 1 h at 45C in 50 µL of silyl 911 (99% BSTFA + 1% TMCS) according to Wubert et al. (1997).…”

Section: Methodsmentioning

confidence: 99%

Lipid and Aroma Composition of Black Cumin (Nigella Sativa L.) Seeds From Tunisia

Sellami¹,

Kchouk

Marzouk

2008

J Food Biochemistry

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The chemical composition of Tunisian PRACTICAL APPLICATIONSThe results obtained showed that Nigella sativa L. seeds were a good source of polyunsaturated fatty acids (PUFAs), phytosterols (PSs) and phospholipids (PhLs) for the human diet. These seeds could be used by the food industry for formulating functional foods enriched with PUFAs and PSs. For pharmaceutical applications, N. sativa L. conjugated sterols could be used as precursors for the hemisynthesis of many hydrosoluble steroids. Also, N. sativa L. seeds are a good source of PhLs and aroma compounds, and therefore, they 1 Corresponding

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Section: Methodsmentioning

confidence: 99%

Lipid and Aroma Composition of Black Cumin (Nigella Sativa L.) Seeds From Tunisia

Sellami¹,

Kchouk

Marzouk

2008

J Food Biochemistry

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“…Optimal separation of the three analytes was achieved with the following oven program: initial temperature, 150˚C for 1 min, followed by an increase to 280˚C at 8˚C/min, with a final time of 20 min. Specific ions were used for selected ion monitoring (54).…”

Section: Characterization Of Compoundsmentioning

confidence: 99%

Ginger Phenylpropanoids Inhibit IL-1β and Prostanoid Secretion and Disrupt Arachidonate-Phospholipid Remodeling by Targeting Phospholipases A2

Nievergelt

Marazzi

Schoop³

et al. 2011

The Journal of Immunology

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The rhizome of ginger (Zingiber officinale) is employed in Asian traditional medicine to treat mild forms of rheumatoid arthritis and fever. We have profiled ginger constituents for robust effects on proinflammatory signaling and cytokine expression in a validated assay using human whole blood. Independent of the stimulus used (LPS, PMA, anti-CD28 Ab, anti-CD3 Ab, and thapsigargin), ginger constituents potently and specifically inhibited IL-1β expression in monocytes/macrophages. Both the calcium-independent phospholipase A2 (iPLA2)-triggered maturation and the cytosolic phospholipase A2 (cPLA2)-dependent secretion of IL-1β from isolated human monocytes were inhibited. In a fluorescence-coupled PLA2 assay, most major ginger phenylpropanoids directly inhibited i/cPLA2 from U937 macrophages, but not hog pancreas secretory phospholipase A2. The effects of the ginger constituents were additive and the potency comparable to the mechanism-based inhibitor bromoenol lactone for iPLA2 and methyl arachidonyl fluorophosphonate for cPLA2, with 10-gingerol/-shogaol being most effective. Furthermore, a ginger extract (2 μg/ml) and 10-shogaol (2 μM) potently inhibited the release of PGE2 and thromboxane B2 (>50%) and partially also leukotriene B4 in LPS-stimulated macrophages. Intriguingly, the total cellular arachidonic acid was increased 2- to 3-fold in U937 cells under all experimental conditions. Our data show that the concurrent inhibition of iPLA2 and prostanoid production causes an accumulation of free intracellular arachidonic acid by disrupting the phospholipid deacylation-reacylation cycle. The inhibition of i/cPLA2, the resulting attenuation of IL-1β secretion, and the simultaneous inhibition of prostanoid production by common ginger phenylpropanoids uncover a new anti-inflammatory molecular mechanism of dietary ginger that may be exploited therapeutically.

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“…Their measurement represents one of the most reliable approaches to assess oxidative stress status in vivo [81]. Several methods are used to measure F 2 -isoprostanes: gas chromatography/mass spectrometry [12, 21, 82, 83, 84, 85, 86, 87, 88]; gas chromatography/tandem mass spectrometry [89, 90]or liquid chromatography/tandem mass spectrometry [24]. The main problems with these specific methods are their cost and their technology which limit their routine use.…”

Section: Isoprostane Formation In Vascular Diseasesmentioning

confidence: 99%

Vascular Biology of the Isoprostanes

Cracowski¹,

Devillier²,

Stanke‐Labesque³

et al. 2001

J Vasc Res

101

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Isoprostanes are a family of compounds produced from polyunsaturated fatty acids via a free-radical-catalysed mechanism. F₂-isoprostanes are prostaglandin F_2α isomers derived from arachidonic acid. These compounds induce potent vasoconstriction, mediated primarily by TP receptor stimulation, and in some vessels by the release of cyclooxygenase products. This vasoconstriction may be modulated by the endothelium through the release of NO. Potent vasoconstriction is also observed with E₂-isoprostanes. Experimental and clinical data suggest a role for F₂-isoprostanes in atherogenesis. These compounds can be detected in free forms in biological fluids as well as esterified in low-density lipoproteins or cell membranes. Their quantification represents a reliable marker of lipid peroxidation. Elevated levels of F₂-isoprostanes in biological fluids in pathological conditions including atherosclerosis, ischaemia-reperfusion injury, and inflammatory vascular diseases, suggest a relationship between lipid peroxidation and such diseases. F₂-isoprostanes are currently being investigated as non-invasive quantitative markers to monitor the response to anti-oxidant treatment.

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Simultaneous Solid Phase Extraction, Derivatization, and Gas Chromatographic Mass Spectrometric Quantification of Thromboxane and Prostacyclin Metabolites, Prostaglandins, and Isoprostanes in Urine

Cited by 27 publications

References 22 publications

Lipid and Aroma Composition of Black Cumin (Nigella Sativa L.) Seeds From Tunisia

Lipid and Aroma Composition of Black Cumin (Nigella Sativa L.) Seeds From Tunisia

Ginger Phenylpropanoids Inhibit IL-1β and Prostanoid Secretion and Disrupt Arachidonate-Phospholipid Remodeling by Targeting Phospholipases A2

Vascular Biology of the Isoprostanes

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