Simultaneous spectrophotometric determination of drug components from their dosage formulations

Gupta, Deeksha; Bhardwaj, Shruti; Sethi, Sonika; Pramanik, Susmita; Das, Dipak K.; Kumar, Rajender; Singh, Prabal Pratap; Vashistha, Vinod Kumar

doi:10.1016/j.saa.2021.120819

Cited by 25 publications

(6 citation statements)

References 57 publications

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“…For the determination of Metformin Hydrochloride and Pravastatin sodium, we will utilize their isoabsorptive point at 246 nm. By the analysis of the recorded absorbance at the isoabsorptive point, the absorbance corresponding to Metformin Hydrochloride or Pravastatin sodium, separately, at isoabsorptive point 246 nm can be calculated using absorbance factor [abs 246 / abs 232] which is the average of the absorbance of different concentrations of pure Metformin Hydrochloride using isoabsorptive point at 246 nm to that at 232 nm which shows no contribution of Pravastatin Sodium and then the absorbance of Metformin Hydrochloride and can be obtained after subtraction [17]. Where abs ʎ Metformin Hydrochloride + Pravastatin Sodium is the absorbance of the binary mixture at 232 nm and abs 246, abs232 is the absorbance factor of pure Metformin Hydrochloride at 246 nm to 232 nm and it was calculated and found to be 0.38.…”

Section: Absorbance Subtraction Methodsmentioning

confidence: 99%

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Development and Validation of UV-Spectroscopic Method for Simultaneous Estimation of Metformin Hydrochloride and Pravastatin Sodium

SHARMA

Verma

Kumar

et al. 2023

J. Pharm. Res. Int.

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Introduction: A new, simple, precise, accurate, and reproducible method was developed and validated for the simultaneous estimation of Metformin Hydrochloride (MH) and Pravastatin Sodium (PS) in pure form. Methodology: Simultaneous estimation of Metformin Hydrochloride (MH) and Pravastatin Sodium (PS) was estimated by ultraviolet (UV) spectrophotometry using the absorbance subtraction method. The method was based on the measurement of absorbance at two wavelengths 232 nm and 238 nm, of metformin and Pravastatin Sodium respectively. These studies were performed at three different levels (75%, 100%, and 125%) and the % recovery of MH and PS was calculated. Results: The LOD and LOQ were found to be 0.481 μg/ml and 0.670μg/ml for MH and 1.15μg/ml and 1.68μg/ml for PS respectively. All the statically analyses were within the standard limits. It proves that the method was repeatable and selective for the simultaneous. Conclusion: Therefore, the present study concludes that it can be successfully used for simultaneous estimation of MH and PS in pure and pharmaceutical dosage forms. The developed method was found to be simple, precise, and accurate.

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Section: Absorbance Subtraction Methodsmentioning

confidence: 99%

“…The advantage of the absorbance subtraction method (AS) over the conventional isoabsorptive point is that there is no need for another complementary spectrophotometric method to measure the concentration of one of the two components to get the second by subtraction [17,18].…”

Section: Absorbance Subtraction Methodsmentioning

confidence: 99%

Development and Validation of UV-Spectroscopic Method for Simultaneous Estimation of Metformin Hydrochloride and Pravastatin Sodium

SHARMA

Verma

Kumar

et al. 2023

J. Pharm. Res. Int.

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“…These reactions have also been successfully used for the micro-level quantification of employed catalysts and drugs/compounds that can bind strongly with a catalyst [22][23][24]. Several reports are available on the spectrophotometric determination of various drugs in pharmaceutical preparations [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Kinetic Study of Ru(III) Promoted Oxidation of L-Tryptophan in an Anionic Surfactant Medium by Hexacyanoferrate(III)

et al. 2023

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The kinetic investigation of Ru(III) promoted oxidation of L-tryptophan (Trp) by [Fe(CN)6]3- has been performed in anionic sodium dodecyl sulfate (SDS) micellar medium by recording the decrease in absorbance at 420 nm, corresponding to [Fe(CN)6]3- using UV-visible spectrophotometer. Pseudo-first-order condition has been used to examine the progress of reaction as a function of [Fe(CN)63−], ionic strength, [OH-], [SDS], [Ru3+], [Trp], and temperature by changing one variable at a time. The results exhibit that [OH-], [SDS], and [Trp] are the decisive parameter showing an appreciable effect on reaction rate. The reaction exhibits first-order kinetics in the studies concentration range of Ru(III), [Fe(CN)6]3− and at lower [Trp] and [OH-]. The incremental trend observed in the reaction rate with electrolyte concentration shows a positive salt effect. The reaction rate is almost ten times faster in SDS micellar medium compared to the aqueous medium. [Fe(CN)6]3- does not show any appreciable effect on the critical micellar concentration (CMC) of SDS as the polar head of SDS and [Fe(CN)6]3- both are negatively charged. The K+ obtained from K3[Fe(CN)6] and KNO3 decreases the repulsion between the negatively charged heads of the surfactant molecules thereby decreasing the CMC of SDS. The activation parameters also support the outer-sphere electron transfer mechanism as proposed by us. Resumen. El estudio cinético de la oxidación de L-tryptofano (Trp) con [Fe(CN)6]3- asistida por Ru(III), se llevó a cabo en un medio micelar de dodecilsulfato de sodio aniónico (SDS) y se monitoreó utilizando espectrometría de UV-visible midiendo la disminución de la absorbancia a 420 nm, correspondiente al [Fe(CN)6]3-. Para examinar el avance de la reaccción se utilizaron condiciones de pseudo-primer orden en función de [Fe(CN)63−], fuerza iónica, [OH-], [SDS], [Ru3+], [Trp], y temperatura, variando siempre una sola una variable. Los resultados indican, que los parametros decisivos que tuvieron un efecto apreciable sobre la velocidad de la reacción son [OH-], [SDS], y [Trp]. La reacción sigue una cinética de primer orden en el rango de concentraciones de estudio de Ru(III), [Fe(CN)6]3− y a bajas concentraciones de [Trp] y [OH-]. La tendencia de incremento de velocidad de la reacción con aumento de la concentración del electrolito muestra un efecto salino positivo. La velocidad de la reacción en el medio micelar de SDS es casi diez veces mayor que en solución acuosa. [Fe(CN)6]3- no muestra ningún efecto appreciable en la concentración crítica micelar (CMC) de SDS debido a que el grupo polar del SDS (SO3-, cabeza) y el [Fe(CN)6]3- tienen ambos carga negativa. Los cationes K+ provenientes del K3[Fe(CN)6] y KNO3 disminuyen la repulsión entre las cabezas con cargas negativas del surfactante, bajando así la CMC del SDS. Los parámetros de activación apoyan también el mecanismo de transferencia de electrones de la esfera exterior propuesto.

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“…In recent years, advancements have been made in spectrophotometric methods for the determination of drugs in pharmaceutical preparations. These new methods have been developed to improve the accuracy and precision of drug analysis and to reduce the time and cost involved in analysis (Gupta et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Determination of Glycyrrhizic Acid by Methyl Violet Dual-wavelength Superimposed Visible Spectrophotometry and its Analytical Applications

Wen

Huang

Tian

et al. 2023

Preprint

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An indirect determination method for glycyrrhizic acid in health foods was established using a dual-wavelength superposition visible spectrophotometric technique. The method relies on the reaction between diammonium glycyrrhizinate and the cationic dye methyl violet. In Clark-Lubs buffer solution at pH 3.0, diammonium glycyrrhizinate transforms into glycyrrhizinate, forming supramolecular aggregates with methyl violet through hydrogen bonding. This results in positive and negative absorption peaks in the visible region, with the maximum positive absorption occurring at 564 nm and the sub-maximum negative absorption at 676 nm. The respective molar absorption coefficients are 2.77×105 L/(mol·cm) and 1.53×105 L/(mol·cm). However, when utilizing the dual-wavelength superposition method, the molar absorption coefficient becomes 4.28×105 L/(mol·cm). The linear range for diammonium glycyrrhizinate determination is 1.00–2.50 mg/L, with a correlation coefficient of 0.9997 and a detection limit of 0.54 mg/L. This proposed method offers an indirect means to determine the glycyrrhizic acid in health foods. The measured values' relative standard deviation (n = 6) was consistently within 5%, indicating satisfactory results.

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Simultaneous spectrophotometric determination of drug components from their dosage formulations

Cited by 25 publications

References 57 publications

Development and Validation of UV-Spectroscopic Method for Simultaneous Estimation of Metformin Hydrochloride and Pravastatin Sodium

Development and Validation of UV-Spectroscopic Method for Simultaneous Estimation of Metformin Hydrochloride and Pravastatin Sodium

Kinetic Study of Ru(III) Promoted Oxidation of L-Tryptophan in an Anionic Surfactant Medium by Hexacyanoferrate(III)

Determination of Glycyrrhizic Acid by Methyl Violet Dual-wavelength Superimposed Visible Spectrophotometry and its Analytical Applications

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