Simultaneous stereoselective analysis of venlafaxine and O-desmethylvenlafaxine enantiomers in human plasma by HPLC-ESI/MS using a vancomycin chiral column

Li, Wen; Wang, Feng; Huan-de, LI

doi:10.1016/j.jchromb.2006.11.021

Cited by 47 publications

(22 citation statements)

References 18 publications

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“…Although some methods have been published using gas chromatography [8,9], measurements have been mainly performed by HPLC coupled with UV detection [10][11][12], and with mass spectrometry MS [13] and MS-MS [14,15].…”

Section: Introductionmentioning

confidence: 99%

A reliable and rapid tool for plasma quantification of 18 psychotropic drugs by ESI tandem mass spectrometry

Vecchione¹,

Casetta²,

Chiapparino³

et al. 2012

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

A reliable and rapid tool for plasma quantification of 18 psychotropic drugs by ESI tandem mass spectrometry

Vecchione¹,

Casetta²,

Chiapparino³

et al. 2012

Journal of Pharmaceutical and Biomedical Analysis

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“…All LC-MS/MS determinations are having good sensitivity. [22,[31][32][33][34][35]38,40,44,45] GC-NPD [47] and some methods with fluorescence [22,45] detection also have appreciable sensitivity. Five different methods were developed for the determination of VX alone in different pharmaceutical formulations.…”

Section: Chromatographymentioning

confidence: 99%

“…HPLC-UV methods are convenient and more economical than LC-MS methods, but are less sensitive. Quantitation limits of HPLC-UV methods [23,24,[26][27][28]30,37,39,41,46] varies between 0.05 -600 µg/ml, whereas this range is 0.1 -5.0 ng/ml for LC-MS systems [22,[31][32][33][34][35]38,40,44,45] clearly demonstrated the sensitivity comparison of UV and MS detection.…”

Section: Chromatographymentioning

confidence: 99%

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Analytical methods for venlaflaxine hydrochloride and metabolites determinations in different matrices

Shrivastava¹

2012

Syst Rev Pharm

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A B S T R A C TThere may be significant variation in stereoisomers related to distribution, bioavailability, metabolism, and excretion. Venlafaxine is a bicyclic phenylethylamine derivative, a unique antidepressant,which structurally differs from other currently available anti-depressants. Its mechanism of action is believed to be associated with triggering neurotransmitter activity in the CNS. This drug is a racemic mixture of the (−)-R-enantiomer and (+)-S-enantiomer. O-desmethylvenlaflaxine is the major metabolite formed after first pass metabolism with similar potency as parent drug. Two less potent minor metabolites are N-desmethylvenlafaxine and N,O-didesmethylvenlafaxine. Thus, summarization of all of the analytical methods for the determination of venlaflaxine alone, in combination, or in the presence of other metabolites, is summarized. The keywords used for search were "Analytical methods on Venlaflaxine," "Determination of venlaflaxine," "Determination of venlaflaxine and metabolites," and "Venlaflaxine plasma determinations." Eight spectrophotometry, 26 chromatography, and seven electroanalytical methods were found for the determination of venlaflaxine alone, in formulations, biological matrices, and simultaneous determinations with metabolites and other anti-depressants. Short review of pharmacological activity of venlaflaxine and its metabolites are also presented.

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“…Methods capable of separating enantiomers of venlafaxine and O-desmethylvenlafaxine have been reported based on capillary electrophoresis [5][6][7][8][9][10][11][12][13] and HPLC-ESI/MS [14]. Thus, the aim of the present work was to establish a simple, accurate, rapid and sensitive LC-MS/MS method with a lower limit of quantification (LLOQ) of 0.5 ng/mL using a very low volume (50μL) of rabbit plasma.…”

Section: Introductionmentioning

confidence: 99%

A Highly Sensitive LC-Ms/Ms Method for Determination of Desvenlafaxine in Rabbit Plasma and its Application to Rabbit Pharmacokinetic Study

Reddy¹

2011

J Anal Bioanal Tech

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A highly senstive and rapid LC-MS/MS method has been developed and validated for the estimation of desvenlafaxine in rabbit plasma. The chromatographic separation was performed with 0.2% formic acid: methanol at flow rate of 0.4 mL/min on Symmetry Shield RP18 column with a total run time of 3.0 min. TheMS/MS ion transitions monitored were 263.90 58.10 for desvenlafaxine and 281.30 86.10 for IS (metaprolol). Method validation and pre-clinical sample analysis were performed as per FDA guide lines and the results met the acceptance crieteria. The lower limit of quantification achieved was 0.5 ng/mL and the linearity was observed from 0.5 to 1500ng/mL. This novel method has been applied to pharmacokinetic study of desvenlafaxine in rabbits. Page 2 of 6 10ASvp) and auto-sampler (SIL-HTC) along with a system controller (SCL-10Avp) was used to inject 2 μL aliquots of the processed samples on a Symmetry Shield RP18 column (50 x 4.6 mm, 3.5 μm, Waters Corporation, Ireland, UK), which was kept at ambient temperature (24 ± 2°C). The isocratic mobile phase, a mixture of 0.2% formic acid and methanol mixture (20:80, v/v) was filtered through a 0.45 μm membrane filter (Millipore) and then degassed ultrasonically for 5 min was delivered at a flow rate of 0.40 mL/min into the mass spectrometer electro spray ionization chamber. Mass spectrometry operating conditionsQuantification was achieved by MS/MS detection in positive ion mode for analyte and IS using a MDS Sciex (Foster City, CA, USA) API 4000 mass spectrometer, equipped with a Turboionspray™ interface at 500°C. The common parameters, i.e. curtain gas, nebulizer gas, auxillary gas and collision gas, were set at 10, 35, 40 and 6 psi, respectively. The compounds parameters, i.e. declustering potential (DP), collision energy (CE), collision exit potential (CXP) and entrance potential (EP) for DVS and IS were 60, 43, 8, 10 V and 44, 26, 8, 10 V, respectively. Detection of the ions was performed in the multiple reaction monitoring (MRM) mode, monitoring the transition of the m/z 263.90 precursor ion to the m/z 58.10 product ion for DVS and m/z 281.30 precursor ion to the m/z 86.10 product ion for IS. Quadrupole Q1 was set on low resolution where as Q3 was set on unit resolution. The analytical data were processed by Analyst software (version 1.4.2). Preparation of stock and standard solutionsPrimary stock solutions of DVS for preparation of standard and quality control (QC) samples were prepared from separate weighing. The primary stock solutions were prepared in methanol (1000 μg/mL). The IS stock solution of 1000 μg/mL was prepared in methanol. The stock solutions of DVS and IS were stored at 4°C, which were found to be stable for one month (data not shown) and successively diluted with methanol to prepare working solutions to prepare the calibration curve (CC). Another set of working stock solutions of DVS were made in methanol (from primary stock) for preparation of QC samples. Working stock solutions were stored at approximately 4°C for a week (data not shown). A...

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Simultaneous stereoselective analysis of venlafaxine and O-desmethylvenlafaxine enantiomers in human plasma by HPLC-ESI/MS using a vancomycin chiral column

Cited by 47 publications

References 18 publications

A reliable and rapid tool for plasma quantification of 18 psychotropic drugs by ESI tandem mass spectrometry

A reliable and rapid tool for plasma quantification of 18 psychotropic drugs by ESI tandem mass spectrometry

Analytical methods for venlaflaxine hydrochloride and metabolites determinations in different matrices

A Highly Sensitive LC-Ms/Ms Method for Determination of Desvenlafaxine in Rabbit Plasma and its Application to Rabbit Pharmacokinetic Study

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