Simultaneous structural and elemental nano-imaging of human brain tissue

Abstract

Examining chemical and structural characteristics of micro-features in complex tissue matrices is essential for understanding biological systems. Advances in multimodal chemical and structural imaging using synchrotron radiation has overcome many...

Altered SOD1 maturation and post-translational modification in amyotrophic lateral sclerosis spinal cord

“…The metal content of SOD1 aggregates in the ventral spinal cord of ALS cases was quantified in fresh frozen tissue sections using XFM at the Australian Synchrotron (Clayton, Victoria, Australia), according to our published method. 27 Briefly, frozen spinal cord tissue blocks from three SOD1 -fALS, one non-SOD1-fALS and one sALS case underwent serial cryosectioning (20 µm) at −20°C. Immunohistochemical identification of SOD1 aggregates was performed on the first of two facing serial sections, using B8H10 conformation-specific SOD1 primary antibody-coupled with DAB staining on a Superfrost™ microscope slide (ThermoFisher Scientific), as described before.…”

“… 30 Images were exported as 32-bit tiff files for further analysis in Fiji software (National Institutes of Health, USA) as previously described. 27 Images were analysed as described previously. 27 In brief, limit of detection for each element was determined and each pixel corrected for background.…”

An integrated mass spectrometry imaging and digital pathology workflow for objective detection of colorectal tumours by unique atomic signatures

“…While it is certainly possible that elemental distribution changes are reflective of an underlying oncogenic pathology, each mechanism must be further interrogated using native-state conditions to preserve chemical state as much as possible. 60 …”

“…While it is certainly possible that elemental distribution changes are reective of an underlying oncogenic pathology, each mechanism must be further interrogated using native-state conditions to preserve chemical state as much as possible. 60 Fixation, dehydration and paraffin embedding undoubtedly and irrevocably alters tissue biochemistry in a way that limits direct interpretation of changes in metal species, and each analyte is affected to differing degrees. However, LA-ICP-MS allows for thorough interrogation of tissue composition in the same way histochemical staining allow for morphological assessment.…”

XRF-ROI-Finder: Machine learning to guide region-of-interest scanning for X-ray fluorescence microscopy

“…The Bionanoprobe (BNP) at beamline 9-ID of the Advanced Photon Source (APS) at Argonne National Laboratory focuses on advancing synchrotron-based X-ray fluorescence (XRF) imaging and elemental mapping techniques for a broad range of biological studies including the imaging of mouse fibroblast cells (Chen et al, 2015), the effect of nanomedicine on rabbit liver tissues via sulfur maps (Deng et al, 2022), a simultaneous qualitative investigation of structural features and quantitative elemental maps of ex vivo tissues (Genoud et al, 2020), specifying discrete zinc-enriched vesicles in oocyte growth and egg fertilization via single-cell level zinc mapping (Que et al, 2015). Facilitation of further scientific discovery at 9-ID beamline as well as other beamlines at APS and their consequent scientific impact is expected to accelerate with the upcoming Advanced Photon Source Upgrade (APS-U) providing higher energy X-rays for faster measurement and higher resolution (Fornek, 2019).…”