Simultaneous UPLC MS/MS analysis of endogenous jasmonic acid, salicylic acid, and their related compounds

Abstract

Jasmonic acid (JA) and salicylic acid (SA) are plant hormones involved in plant growth and development. Recent studies demonstrated that presence of a complex interplay between JA and SA signaling pathways to response to pathogenesis attack and biotic stresses. To our best knowledge, no method has existed for simultaneous analyses of JA, SA, and their related compounds. Especially, the glucosides are thought to be the storages or the inactivated compounds, but their contribution should be considered for elucidating the amount of the aglycons. It is also valuable for measuring the endogenous amount of phenylalanine, cinnamic acid, and benzoic acid that are the biosynthetic intermediates of SA due to the existence of isochorismate pathway to synthesize SA. We established this method using deuterium labeled compounds as internal standards. This is the first report of simultaneous analysis of endogenous JA, SA, and their related compounds. Measuring the endogenous JA, SA, and their related compounds that had been accumulated in tobacco plants proved the practicality of the newly developed method. It was demonstrated that accumulation of JA, SA and their related compounds were induced in both case of TMV infection and abiotic stresses.

Soybean Metabolites Regulated in Root Hairs in Response to the Symbiotic BacteriumBradyrhizobium japonicum

“…Benzoic acid itself exhibits antifungal activity, and pretreatment of plants with this compound increases plant resistance to fungi (Khan et al, 2001;Williams et al, 2003;Shabana et al, 2008). Similarly, benzoic acid levels increase in response to both bacterial and viral infection (Shapiro and Gutsche, 2003;Matsuura et al, 2009). The level of benzoic acid was also shown to increase in plants treated with manganese and cadmium and was correlated with improved plant resistance to cold, drought, and heat Pal et al, 2005;Fuhrs et al, 2009).…”

Cellulase Applied to the Leaves of Sweet Pepper (Capsicum annuumL. var.grossum) Upregulates the Production of Salicylic and Azelaic Acids

“…Young leaves (five or six leaves per plant, weighing approximately 1 g) were harvested 24 h after the third treatment and immediately frozen in liquid nitrogen. A leaf extract was prepared and analyzed as described 6) with some modifications. Briefly, the plant material was crushed and soaked for 12 h in 10 mL of ethanol containing 1% acetic acid.…”

“…After evaporating the solvent, the resulting concentrate was redissolved in 500 mL of a solution of 80% methanol/20% water/0.05% acetic acid (v/v/v) and analyzed by ultra-HPLC (UHPLC) MS/MS in the multiple reaction monitoring mode (MRM). 6) The conditions for UHPLC MS/MS and parameters for MRM were as described in a previous paper, 6) other than those for AzA (3). The MRM parameters for respectively analyzing AzA (3) and AzA-d 14 were as follow: pseudo molecular ion ½M À H À1 , m=z 187.10 and 201.18; transition ion, m=z 124.54 and 137.10; cone voltage, 26 and 26 V; collision energy, 12 and 12 eV.…”

Development and Validation of an Ultra Performance Liquid Chromatography Method for the Determination of Dexketoprofen Trometamol, Salicylic Acid and Diclofenac Sodium

“…22,23,24,25 The gradient mobile phase flow was almost same with dexketoprofen analysis. The temperature was 25°C.…”