Simultaneous Visualization of Endogenous Homocysteine, Cysteine, Glutathione, and their Transformation through Different Fluorescence Channels

Abstract

Studying numerous biologically important species simultaneously is crucial to understanding cellular functions and the root causes of related diseases.D irect visualization of endogenous biothiols in biological systems is of great value to understanding their biological roles.H erein, an ovel multisignal fluorescent probe was rationally designed and exploited for the simultaneous sensing of homocysteine (Hcy), cysteine (Cys), and glutathione (GSH) using different emission channels.T his probe was successfully applied to the simultaneous discrimination between and visualization of endogenous Hcy, Cys,GSH, and their transformation in living cells.

Simultaneous multi-signal quantification for highly precise serodiagnosis utilizing a rationally constructed platform

“…The development of a simultaneous multi-signal method to quantify bio-indicators should counter the disadvantages of single-signal methods and provide much more information on the bio-indicator concentration in a single process 7–9 . This will significantly reduce the false-positive and negative rates in medical serodiagnostics.…”

Simultaneous detection of small molecule thiols with a simple 19F NMR platform

“…Both PSP-4F and PSBN-4F outstood among these candidates because of their short reaction times (<3 min). Owing to the strongest electron-decient ability, PSBN-4F displayed a very complicated 19 F NMR spectrum upon the addition of Cys due to the activated F-C bonds and the special S / N rearrangement, [22][23][24][25] while PSP-4F formed a stable product and exhibited two explicit peaks under the same conditions, which was much more straightforward for further analysis. Besides suitable reactivity, PSP-4F possessing two types of environmentally different 19 F atoms also showed superior recognition ability towards thiols ( Fig.…”

Nucleophilie von Glutathion als Bindeglied zur Reaktivität von Michael‐Akzeptoren

“…Analog wurde die Elektrophilie E von 72 weiteren Michael‐Akzeptoren in einem Reaktivitätsbereich von −14.4 ≤ E ≤ −25.3 abgeschätzt . Verfügbare Geschwindigkeitskonstanten eröffnen außerdem die Möglichkeit, die kürzlich entwickelten dynamischen GSH‐Fluoreszenzsonden M74 – M82 in die Elektrophilieskalen nach Mayr zu integrieren (Schema ) …”