Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems

Ahmerkamp, Soeren; Jalaluddin, Farooq  M.; Cui, Yuan; Brumley, Douglas R.; Pacherres, César O.; Berg, Jerry S. Vande; Stocker, Roman; Kuypers, Marcel M. M.; Koren, Klaus; Behrendt, Lars

doi:10.1016/j.crmeth.2022.100216

Cited by 20 publications

(12 citation statements)

References 93 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During our measurements of some individual particles, we noticed a substantial alteration of porosity towards lower values, which may introduce some uncertainty. Additionally, recent publications have highlighted the significant impact of ocean stratification (beyond pycnoclines) on particle settling velocity, influenced by drag enhancement and delayed buoyancy adaptation (Ahmerkamp et al 2022). These processes are likely to further shift carbon fluxes towards smaller particles and should be taken into consideration in future studies.…”

Section: Discussionmentioning

confidence: 99%

Rethinking sinking: Imaging flow fields of natural marine aggregates to infer porosity-dependent changes in sinking velocity and carbon flux

Flintrop,

Ahmerkamp,

Moradi

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

The marine biological carbon pump is driven by the interplay between particle sinking velocity and remineralisation. Despite its importance, sinking velocity of natural marine aggregates is not routinely measured, but often calculated from aggregate size and density using Stokes′ law. Yet, comparing calculated and experimentally measured sinking velocities has shown that Stokes′ law does not accurately predict the size-to-sinking relationship of marine aggregates because size-dependent changes in porosity are not sufficiently taken into account. To determine the factors controlling the sinking of marine aggregates and to establish a better scaling including a porosity term, we analysed the flow fields around 81 in situ collected aggregates using Particle Image Velocimetry. Additionally, we measured the size, sinking velocity, density and porosity of individual aggregates. The flow fields around the aggregates strongly resembled those of impermeable, porous spheres, independent of aggregate type, size, composition, and shape. Using an independently derived scaling of porosity with size, we could accurately predict the sinking velocity of laboratory- and field-collected aggregates with known density, which led to a 2-fold improvement in carbon flux predictions, compared to sinking velocity calculated with Stokes′ drag only. Furthermore, we found that small particles (<524 μm) account for a remarkable 80% of the total carbon flux in the sampling area. Using a comprehensive global in situ particle dataset, we demonstrate that this trend persists consistently across various oceans with small geographical variations, ranging from 40% in high latitudes to 70% in equatorial regions. It is established that small particles contribute disproportionately to carbon export fluxes because of their high numerical abundances. Here, we provide an additional explanation, which is that their importance can primarily be attributed to their relatively low porosities and high carbon-to-volume ratio.

show abstract

Section: Discussionmentioning

confidence: 99%

Rethinking sinking: Imaging flow fields of natural marine aggregates to infer porosity-dependent changes in sinking velocity and carbon flux

Flintrop,

Ahmerkamp,

Moradi

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Particle image velocimetry. A very different approach toward multiparameter sensing was taken by Ahmerkamp et al 70 This team combined particle image velocimetry (PIV) with O 2sensitive particles (Fig. 9).…”

Section: External Stimulimentioning

confidence: 99%

Optical sensors (optodes) for multiparameter chemical imaging: classification, challenges, and prospects

Kalinichev,

Zieger,

Koren

2024

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

“…In order to relate the heterogeneous Chla distribution inside the tissue with the O 2 dynamics at the DBL, we used particle image velocimetry (PIV) using O 2 -sensitive nanoparticles in an image velocimetry system (sensPIV) 50 (Figure 2A). We observed a heterogeneous O 2 distribution along the coral surface.…”

Section: Flow Field and O 2 Concentrations At The Coral Surfacementioning

confidence: 99%

“…To resolve the O 2 and flow field around the coral fragment, we used O 2 sensitive nanoparticles, 78 which have proven ideal for mapping O 2 concentrations at relevant scales. 50 The stock solution contained 2 mg mL -1 which was further diluted by a factor of 200. Illumination was achieved using a LED pulsing system (LPS3, iLA_5150, Germany) connected to LED light sheet optics similar to the one described by Willert et al 79 The light sheet was approx.…”

Section: Senspiv Measurementsmentioning

confidence: 99%

Ciliary flows in corals ventilate target areas of high photosynthetic oxygen production

Pacherres¹,

Ahmerkamp²,

Koren³

et al. 2022

Current Biology

Self Cite

View full text Add to dashboard Cite

Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems

Cited by 20 publications

References 93 publications

Rethinking sinking: Imaging flow fields of natural marine aggregates to infer porosity-dependent changes in sinking velocity and carbon flux

Rethinking sinking: Imaging flow fields of natural marine aggregates to infer porosity-dependent changes in sinking velocity and carbon flux

Optical sensors (optodes) for multiparameter chemical imaging: classification, challenges, and prospects

Ciliary flows in corals ventilate target areas of high photosynthetic oxygen production

Contact Info

Product

Resources

About