Simultaneous voltammetric immunodetection of alpha-fetoprotein and glypican-3 using a glassy carbon electrode modified with magnetite-conjugated dendrimers

Chikhaliwala, Priyal; Rai, Rakesh; Chandra, Sudeshna

doi:10.1007/s00604-019-3354-4

Cited by 31 publications

(14 citation statements)

References 34 publications

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“…Most of the earlier reports for stem cell imaging and tracking have included commercial IONPs which have been discontinued due to safety concerns. To address this problem, our group had previously demonstrated the feasibility of magnetic nanoparticles for stem cell labeling and imaging in vitro (Joseph et al, 2015; Sanganeria, Chandra, 2015; Sanganeria, Sachar et al, 2015; Shelat et al, 2018; Shelat et al, 2019; Chikhaliwala et al, 2019). However, we have utilized l‐arginine and l‐histidine, which have not illustrated considerable signal intensity during in vitro MR scans.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover; osteochondral tissue is avascular which reduces the host immune response (Bahadar et al, 2016). Further, in this study, we have utilized the minimum dose of human BMSCs (1 × 10 [Chikhaliwala et al, 2019]) along with agarose as a stem cell implantation matrix (Khurana et al, 2013). Literature has revealed poor regeneration ability of MSCs due to nonintegration when delivered subcutaneously or intraarticularly (Henning et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Our group has been actively working on the development of biocompatible magnetic nanoparticles for various biomedical applications (Joseph, Sachar, Kishore, & Chandra, 2015; Sanganeria, Chandra, Bahadur, & Khanna, 2015; Sanganeria, Sachar et al, 2015; Shelat, Chandra, & Khanna, 2018; Shelat, Bhatt, Khanna, & Chandra, 2019; Chikhaliwala, Rai, & Chandra, 2019) with major emphasis on their use as MR contrast agents. Even though many of the nanoparticle systems have proved excellent for in vitro studies, they were not utilized ahead for in vivo work due to lack of stability, low iron content, and bulky size which may hamper the labeling of stem cells and subsequent imaging results.…”

Section: Introductionmentioning

confidence: 99%

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Regeneration of hyaline cartilage in osteochondral lesion model using L‐lysine magnetic nanoparticles labeled mesenchymal stem cells and their in vivo imaging

Shelat

Bhatt

Paunipagar

et al. 2020

J Tissue Eng Regen Med

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Treatment of osteochondral defects continues to pose a major challenge for patients and orthopedic surgeons due to the limited healing potential of articular cartilage. Mesenchymal stem cells (MSCs) possess therapeutic potential for the treatment of osteochondral pain and pathology. However, it is necessary to use proper labeling and imaging agent of stem cells that can decipher its role posttransplantation. A major limitation of routinely used contrast agents is signal dilution over a period of time which limits its use for further studies. At the same time, regeneration of fibrocartilage over native hyaline cartilage also limits the use of conventional therapies. The present study evaluates the efficacy of bone marrow-derived mesenchymal stem cells (BMSCs) for the treatment of osteochondral defect in rats with the regeneration of hyaline cartilage in situ and in vivo monitoring of the stem cells using L-lysine functionalized magnetic iron oxide nanoparticles (lys-IONPs). L-lysine stabilizes the iron oxide nanoparticles, enhances the biocompatibility, and provides functionalities for efficient stem cell labeling. in vitro toxic effects of lys-IONPs on mitochondrial impairment, morphological alterations, and actin cytoskeleton reveal minimum damage to BM-MSCs. Histological data (H and E, Masson's trichrome and immunohistochemistry) describe the early initiation of healing and regeneration of hyaline-like cartilage over fibrocartilage in stem cell treated groups. MR scans demonstrate generation of hypointense signals in lys-IONPs-BMSCs with improved signal intensity and minimum loss over 28 days revealing its use as a long-term stem cell labeling and imaging agent. K E Y W O R D S bone marrow mesenchymal stem cells, iron oxide nanoparticles, L-lysine, magnetic resonance imaging, osteochondral defect 1 | INTRODUCTION Osteochondral defects in the knee joint remain to be the most challenging clinical problem for orthopedic surgeons with maximum disability in young athletes and older people (Kumbhar et al., 2017). The overall prevalence rate of osteochondral defect is found to be higher in males than in females (3:1) with young adults at the highest risk (Weiss et al., 2016). Owing to the restricted therapeutic ability of articular cartilage, it is complicated to treat osteochondral defects, leading to disabling pain and early osteoarthritis (Perera, Gikas, & Bentley, 2012). Thus,

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regeneration of hyaline cartilage in osteochondral lesion model using L‐lysine magnetic nanoparticles labeled mesenchymal stem cells and their in vivo imaging

Shelat

Bhatt

Paunipagar

et al. 2020

J Tissue Eng Regen Med

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“…The abnormal liver or circulating Wnt3a in HCC has provided initial evidence, and the tumor volume after intervening in Wnt3a mRNA transcription with specific shRNA was 355.0 ± 99.9 mm 3 in the intervention group which was significantly lower than that (869.4 ± 222.5 mm 3 ) in the negative group, and the time to tumor formation in the intervention group was longer than that in the negative group; the tumor weight (0.35 ± 0.11 g) in the intervention group was markedly lower than that (0.88 ± 0.20 g) in the negative group. Immunohistochemistry confirmed that Wnt3a was strongly inhibited in the intervention group[ 56 ], and indicated that targeted-Wnt3a signaling could result in effective inhibition of HCC growth.…”

Section: Wnt3amentioning

confidence: 99%

Encouraging specific biomarkers-based therapeutic strategies for hepatocellular carcinoma

Yao¹,

Yang²,

Wang³

et al. 2022

WJCC

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The prevention, early discovery and effective treatment of patients with hepatocellular carcinoma (HCC) remain a global medical challenge. At present, HCC is still mainly treated by surgery, supplemented by vascular embolization, radio frequency, radiotherapy, chemotherapy and biotherapy. The application of multikinase inhibitor sorafenib, chimeric antigen receptor T cells, or PD-1/PD-L1 inhibitors can prolong the median survival of HCC patients. However, the treatment efficacy is still unsatisfactory due to HCC metastasis and postoperative recurrence. During the process of hepatocyte malignant transformation, HCC tissues can express and secrete many types of specific biomarkers, or oncogenic antigen molecules into blood, for example, alpha-fetoprotein, glypican-3, Wnt3a (one of the key signaling molecules in the Wnt/β-catenin pathway), insulin-like growth factor (IGF)-II or IGF-I receptor, vascular endothelial growth factor, secretory clusterin and so on. In addition, combining immunotherapy with non-coding RNAs might improve anti-cancer efficacy. These biomarkers not only contribute to HCC diagnosis or prognosis, but may also become molecular targets for HCC therapy under developing or clinical trials. This article reviews the progress in emerging biomarkers in basic research or clinical trials for HCC immunotherapy.

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“…The molecular weight for D5, D10, D8 and D16 PAMAM dendrimers were 673, 1813, 1428 and 3092 g/mol, respectively. All these four dendrimer systems were functionalized to Fe 3 O 4 NPs by modified chemical in-situ co-precipitation method [43]. The precursors Fe 2 + and Fe 3 + ions were taken in a molar ratio of 1 : 2 and mechanically stirred at 400 rpm for 20 min.…”

Section: Synthesis Of Pamam@fe 3 O 4 Npsmentioning

confidence: 99%

Poly‐amidoamine Dendrimers@Fe₃O₄ Based Electrochemiluminescent Nanomaterials for Biosensing of Liver Cancer Biomarkers

Chikhaliwala

Chandra

2020

Electroanalysis

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Electrochemiluminescence (ECL) of poly‐amidoamine (PAMAM) dendrimers functionalized to Fe3O4 nanoparticles (PAMAM@Fe3O4 NPs) based on Ru(bpy)32+ complex is reported. Different generations of PAMAM dendrimer consisted of 5 (D5), 10 (D10), 8 (D8) and 16 (D16) surface primary amine‐terminated groups. D5@Fe3O4 NPs enhanced ECL emission in comparison to others. A possible mechanism is elucidated based on structural functionality of PAMAM with respect to amines, carbonyl, hydroxyl groups and size‐based diffusion. ECL biosensing of alpha‐fetoprotein (AFP) and glypican‐3 (GPC‐3) using 1 mM tri‐propylamine is demonstrated using D5@Fe3O4NP@antibodies as detection probes. Detection limit for AFP and GPC‐3 was 6 pg/mL and 0.03 pg/mL, respectively.

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Simultaneous voltammetric immunodetection of alpha-fetoprotein and glypican-3 using a glassy carbon electrode modified with magnetite-conjugated dendrimers

Cited by 31 publications

References 34 publications

Regeneration of hyaline cartilage in osteochondral lesion model using L‐lysine magnetic nanoparticles labeled mesenchymal stem cells and their in vivo imaging

Regeneration of hyaline cartilage in osteochondral lesion model using L‐lysine magnetic nanoparticles labeled mesenchymal stem cells and their in vivo imaging

Encouraging specific biomarkers-based therapeutic strategies for hepatocellular carcinoma

Poly‐amidoamine Dendrimers@Fe₃O₄ Based Electrochemiluminescent Nanomaterials for Biosensing of Liver Cancer Biomarkers

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Simultaneous voltammetric immunodetection of alpha-fetoprotein and glypican-3 using a glassy carbon electrode modified with magnetite-conjugated dendrimers

Cited by 31 publications

References 34 publications

Regeneration of hyaline cartilage in osteochondral lesion model using L‐lysine magnetic nanoparticles labeled mesenchymal stem cells and their in vivo imaging

Regeneration of hyaline cartilage in osteochondral lesion model using L‐lysine magnetic nanoparticles labeled mesenchymal stem cells and their in vivo imaging

Encouraging specific biomarkers-based therapeutic strategies for hepatocellular carcinoma

Poly‐amidoamine Dendrimers@Fe3O4 Based Electrochemiluminescent Nanomaterials for Biosensing of Liver Cancer Biomarkers

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Product

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Poly‐amidoamine Dendrimers@Fe₃O₄ Based Electrochemiluminescent Nanomaterials for Biosensing of Liver Cancer Biomarkers