Simultant encapsulation of vitamin C and beta-carotene in sesame (<i>Sesamum indicum l.)</i> liposomes

Hudiyanti, Dwi; Fawrin, H; Siahaan, Parsaoran

doi:10.1088/1757-899x/349/1/012014

Cited by 13 publications

(12 citation statements)

References 35 publications

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“…The optimized liposome did not contain cholesterol. Hudiyanti et al [ 57 ] showed that the encapsulation efficiency of β-carotene decreased from 79% to 60% with 0% and 40% cholesterol, respectively, since both molecules competed to be inside the vesicle bilayer. Quinine entrapment by liposomes was also affected by the cholesterol incorporation in the system, where 40% cholesterol produced an EE ranging from 5.6% to 84.07%; decreasing the cholesterol by 10% increased the EE to 88% [ 58 ].…”

Section: Resultsmentioning

confidence: 99%

Liposomes Loaded with Unsaponifiable Matter from Amaranthus hypochondriacus as a Source of Squalene and Carrying Soybean Lunasin Inhibited Melanoma Cells

et al. 2021

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Amaranthus hypochondriacus is a source of molecules with reported health benefits such as antioxidant activity and cancer prevention. The objective of this research was to optimize the conditions for preparing a liposome formulation using amaranth unsaponifiable matter as a source of squalene in order to minimize the particle size and to maximize the encapsulation efficiency of liposomes for carrying and delivering soybean lunasin into melanoma cell lines. Amaranth oil was extracted using supercritical dioxide carbon extraction (55.2 MPa pressure, 80 °C temperature, solvent (CO2)-to-feed (oil) ratio of 20). The extracted oil from amaranth was used to obtain the unsaponifiable enriched content of squalene, which was incorporated into liposomes. A Box–Behnken response surface methodology design was used to optimize the liposome formulation containing the unsaponifiable matter, once liposomes were optimized. Soybean lunasin was loaded into the liposomes and tested on A-375 and B16-F10 melanoma cells. The squalene concentration in the extracted oil was 36.64 ± 0.64 g/ 100 g of oil. The particle size in liposomes was between 115.8 and 163.1 nm; the squalene encapsulation efficiency ranged from 33.14% to 76.08%. The optimized liposome formulation contained 15.27 mg of phospholipids and 1.1 mg of unsaponifiable matter. Cell viability was affected by the liposome formulation with a half-maximum inhibitory concentration (IC50) equivalent to 225 μM in B16-F10 and 215 μM in A-375. The liposomes formulated with lunasin achieved 82.14 ± 3.34% lunasin encapsulation efficiency and improved efficacy by decreasing lunasin IC50 by 31.81% in B16-F10 and by 41.89% in A-375 compared with unencapsulated lunasin.

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Section: Resultsmentioning

confidence: 99%

Liposomes Loaded with Unsaponifiable Matter from Amaranthus hypochondriacus as a Source of Squalene and Carrying Soybean Lunasin Inhibited Melanoma Cells

et al. 2021

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“…The liposomal applications have been studied extensively in dairy products (Laridi et al 2003), in terms of stability of the food components against degradation (Hudiyanti et al 2018) and enhanced delivery action of the antimicrobial peptides (Paula et al 2019). In cheese-making, liposome encapsulated enzymes reduced the cheese ripening time up to 50%.…”

Section: Food Processingmentioning

confidence: 99%

“…In liquid food products, beta carotene has a low chemical stability and low bioavailability due to its hydrophobicity. Introducing liposome techniques may improve the solubility of beta carotene by incorporation of dried phospholipid particles using spray drying (Toniazzo et al 2014) and active loading techniques (Hudiyanti et al 2018) in sesame based liposome ingredients.…”

Section: Curcuminmentioning

confidence: 99%

An overview of liposomal nano-encapsulation techniques and its applications in food and nutraceutical

2020

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Encapsulation in packaging of food ingredients is of great interest at micro and nano levels. It is a distinct process leading to the entrapping of one substance within another material. Lipid oriented encapsulation methods are currently considered as a superior choice for encapsulation of sensitive ingredients, focusing on foods and dietary supplements of hydrophobic and hydrophilic molecules along with bioactive compounds, food ingredients supplementary systems for therapeutic purpose. Liposome and nanoliposome techniques have been widely used in food industry in nutrient enrichment and supplements. It enhances the sensory attributes and shelf life of the food product and serves as an alternative to micro encapsulation. These lipid and water oriented systems have distinguished advantages and provide higher surface area in food processing, which increases product solubility, bioavailability and permits accurate targeting of the encapsulated material to a greater extent in food and nutraceutical production. This review article focuses on nanoliposome, its preparation techniques, advantages and application of nanoliposome in food and nutraceutical process.

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“…Adding cholesterol to the liposome assembly process makes it possible to maintain and even increase the membrane's stability. An adequate amount of cholesterol in liposomes can decrease membrane permeability and increase membrane stifness, enhancing the membrane's stability [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Te liposomes used in drug delivery systems have been studied experimentally and computationally for polar and nonpolar drugs [12][13][14][15][16][17][18][19][20][21]. Computational studies on issues related to experimental studies can confrm or complement relevant data or information acquired experimentally or even simulate trials to conduct future experiments more efciently.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Phospholipid, Cholesterol, Beta-Carotene, and Vitamin C Molecules in Liposome-Based Drug Delivery Systems: An In Silico Study

Hudiyanti

Putri

Hikmahwati

et al. 2023

Advances in Pharmacological and Pharmaceutical Sciences

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This paper investigates the interaction within a liposome-based drug delivery system in silico. Results confirmed that phospholipids, cholesterol, beta-carotene, and vitamin C in the liposome structures interact noncovalently. The formation of noncovalent interactions indicates that the liposomal structures from phospholipid molecules will not result in chemical changes to the drug or any molecules encapsulated within. Noncovalent interactions formed include (i) moderate-strength hydrogen bonds with interaction energies ranging from −73.6434 kJ·mol−1 to −45.6734 kJ·mol−1 and bond lengths ranging from 1.731 Å to 1.827 Å and (ii) van der Waals interactions (induced dipole-induced dipole and induced dipole-dipole interactions) with interaction energies ranging from −4.4735 kJ·mol−1 to −1.5840 kJ·mol−1 and bond lengths ranging from 3.192 Å to 3.742 Å. The studies for several phospholipids with short hydrocarbon chains show that changes in chain length have almost no effect on interaction energy, bond length, and partial atomic charge.

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Simultant encapsulation of vitamin C and beta-carotene in sesame (Sesamum indicum l.) liposomes

Cited by 13 publications

References 35 publications

Liposomes Loaded with Unsaponifiable Matter from Amaranthus hypochondriacus as a Source of Squalene and Carrying Soybean Lunasin Inhibited Melanoma Cells

Liposomes Loaded with Unsaponifiable Matter from Amaranthus hypochondriacus as a Source of Squalene and Carrying Soybean Lunasin Inhibited Melanoma Cells

An overview of liposomal nano-encapsulation techniques and its applications in food and nutraceutical

Interaction of Phospholipid, Cholesterol, Beta-Carotene, and Vitamin C Molecules in Liposome-Based Drug Delivery Systems: An In Silico Study

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