Sinapic Acid Promotes Browning of 3T3-L1 Adipocytes via p38 MAPK/CREB Pathway

Bae, In-Seon; Kim, Sang Hoon

doi:10.1155/2020/5753623

Cited by 22 publications

(15 citation statements)

References 33 publications

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“…Some studies confirmed the positive role of MAPK pathway in browning of adipocytes in 3T3-L1 cells. ( Bae and Kim, 2020 ). Our present study also supported that the augmentation of browning by SFN treatment is involved with the activation of MAPK pathway at the late period of adipogenesis.…”

Section: Discussionmentioning

confidence: 99%

The Protective Effects of Sulforaphane on High-Fat Diet-Induced Obesity in Mice Through Browning of White Fat

Liu

Chen

et al. 2021

Front. Pharmacol.

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Background: Sulforaphane (SFN), an isothiocyanate naturally occurring in cruciferous vegetables, is a potent indirect antioxidant and a promising agent for the control of metabolic disorder disease. The glucose intolerance and adipogenesis induced by diet in rats was inhibited by SFN. Strategies aimed at induction of brown adipose tissue (BAT) could be a potentially useful way to against obesity. However, in vivo protective effect of SFN against obesity by browning white adipocyte has not been reported. Our present study is aimed at evaluation the efficacy of the SFN against the high-fat induced-obesity mice and investigating the potential mechanism.Methods: High-Fat Diet-induced obese female C57BL/6 mice were intraperitoneally injected with SFN (10 mg/kg) daily. Body weight was recorded every 3 days. 30 days later, glucose tolerance test (GTT) and insulin tolerance test (ITT) were performed. At the end of experiment, fat mass were measured and the adipogenesis as well as browning associated genes expression in white adipose tissue (WAT) were determined by RT-qPCR and western blot. Histological examination of the adipose tissue samples were carried out with hematoxylin–eosin (HE) staining and immunofluorescence staining method. In vitro, pre-adipocytes C3H10T1/2 were treated with SFN to investigate the direct effects on adipogenesis.Results: SFN suppressed HFD-induced body weight gain and reduced the size of fat cells in mice. SFN suppressed the expression of key genes in adipogenesis, inhibited lipid accumulation in C3H10T1/2 cells, increased the expression of brown adipocyte-specific markers and mitochondrial biogenesis in vivo and in vitro, and decreased cellular and mitochondrial oxidative stress. These results suggested that SFN, as a nutritional factor, has great potential role in the battle against obesity by inducing the browning of white fat.Conclusion: SFN could significantly decrease the fat mass, and improve glucose metabolism and increase insulin sensitivity of HFD-induced obese mice by promoting the browning of white fat and enhancing the mitochondrial biogenesis in WAT. Our study proves that SFN could serve as a potential medicine in anti-obesity and related diseases.

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Section: Discussionmentioning

confidence: 99%

The Protective Effects of Sulforaphane on High-Fat Diet-Induced Obesity in Mice Through Browning of White Fat

Liu

Chen

et al. 2021

Front. Pharmacol.

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“…WAT browning in mice is stimulated by overexpression of zinc alpha 2 glycoprotein (ZAG) ( 169 ), and in 3T3-L1 white adipocytes ZAG increases the expression of brown fat markers and induces mitochondrial biogenesis via PKA and p38 signaling ( 170 ). The naturally occurring compound cryptotanshinone promotes brown-tissue and beige-cell markers by activating AMPK, p38, and Smad1/5 ( 58 ), whereas sinapic acid achieves the same effect via AMPK, p38, and PGC1 α ( 171 ). Treatment with the non-steroidal anti-inflammatory drug ketoprofen induces browning in 3T3-L1 cells and inguinal WAT, increasing mitochondrial biogenesis through an intricate signaling pathway involving p38, mTORC1, and COX activation ( 172 ).…”

Section: P38 and White Adipose Tissue Browningmentioning

confidence: 99%

Uncovering the Role of p38 Family Members in Adipose Tissue Physiology

et al. 2020

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The complex functions of adipose tissue have been a focus of research interest over the past twenty years. Adipose tissue is not only the main energy storage depot, but also one of the largest endocrine organs in the body and carries out crucial metabolic functions. Moreover, brown and beige adipose depots are major sites of energy expenditure through the activation of adaptive, non-shivering thermogenesis. In recent years, numerous signaling molecules and pathways have emerged as critical regulators of adipose tissue, in both homeostasis and obesity-related disease. Among the best characterized are members of the p38 kinase family. The activity of these kinases has emerged as a key contributor to the biology of the white and brown adipose tissues, and their modulation could provide new therapeutic approaches against obesity. Here, we give an overview of the roles of the distinct p38 family members in adipose tissue, focusing on their actions in adipogenesis, thermogenic activity, and secretory function.

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“…As the biological activities of sinapic acid and its derivatives have been discovered, they have been used in various fields, such as the pharmaceutical industry, food, beverages, and cosmetics (Chen, 2016). Some studies have shown that sinapic acid has many beneficial properties, such as anti‐inflammatory properties (Bin Jardan et al, 2020; Lan et al, 2021; Lee, 2018; Qian et al, 2020), antioxidant (Yang et al, 2019), antihyperuricemic (Ishaq et al, 2020), anticancer (Eroglu et al, 2018; Zhao et al, 2021), and lipid homeostasis regulation (Wu et al, 2021) and promotes lipolysis (Bae & Kim, 2020; Hossain et al, 2020). However, the effects of sinapic acid on HFD‐induced lipid metabolism and its mechanism of action have been less reported.…”

Section: Introductionmentioning

confidence: 99%

Dietary sinapic acid attenuated high‐fat diet‐induced lipid metabolism and oxidative stress in male Syrian hamsters

Wang

Liang

Cao

et al. 2022

Journal of Food Biochemistry

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The current study investigated the effects of sinapic acid on high‐fat diet (HFD)‐induced lipid metabolism and oxidative stress in male Syrian hamsters. Sinapic acid treatment significantly reduced body weight, epididymal fat, and perirenal fat mass in HFD hamsters. Sinapic acid also improved dyslipidemia levels (reducing the serum levels of total cholesterol, triglycerides, and low‐density lipoprotein cholesterol, and increasing the high‐density lipoprotein cholesterol) and increased T‐AOC levels to mitigate oxidative stress injury. Moreover, sinapic acid intervention increased the activations of PPAR‐γ, CPT‐1, and CYP7A1 and decreased the activations of FAS, ACC1, SREBP1, SREBP2, and HMGCR in the livers of HFD hamsters. In addition, sinapic acid intervention also significantly inhibited the intestinal mRNA levels of Srebp2 and Npc1l1 in HFD hamsters. In conclusion, sinapic acid can significantly attenuate abnormal lipid metabolism in the development of HFD‐induced obesity and reduce the level of oxidative stress to exert its anti‐obesity effect. Practical applications Obesity is the main cause of some chronic metabolic syndromes, such as dyslipidemia, nonalcoholic fatty liver disease, diabetes, and hyperuricemia. Searching for new, safe, and effective natural products in weight loss and fat reduction has become one of the hot research topics. As a natural source of simple phenolic acids, sinapic acid is present in fruits, vegetables, and grains and has been indicated to have anti‐inflammatory, antioxidant, antihyperuricemic, lipid homeostasis regulation, and anticancer activities. However, the lipid metabolism‐ and oxidative stress‐regulating activities of sinapic acid are not clear. Here, the current study investigated the lipid metabolism and oxidative stress regulating activities of sinapic acid in male Syrian hamsters fed a high‐fat diet.

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Sinapic Acid Promotes Browning of 3T3-L1 Adipocytes via p38 MAPK/CREB Pathway

Cited by 22 publications

References 33 publications

The Protective Effects of Sulforaphane on High-Fat Diet-Induced Obesity in Mice Through Browning of White Fat

The Protective Effects of Sulforaphane on High-Fat Diet-Induced Obesity in Mice Through Browning of White Fat

Uncovering the Role of p38 Family Members in Adipose Tissue Physiology

Dietary sinapic acid attenuated high‐fat diet‐induced lipid metabolism and oxidative stress in male Syrian hamsters

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