Sinapic acid protects heart against ischemia/reperfusion injury and H9c2 cardiomyoblast cells against oxidative stress

Silambarasan, Thangarasu; Manivannan, Jeganathan; Priya, Mani Krishna; Suganya, Natarajan; Chatterjee, Suvro; Raja, Boobalan

doi:10.1016/j.bbrc.2014.12.022

Cited by 46 publications

(37 citation statements)

References 42 publications

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“…In accordance with this finding, SA normalised lysosomal enzymes including β-glucuronidase and β-galactosidase, as well as cathepsins B and D. Co-treatment with SA also reduced the size of the infarct, protecting the heart from ISO-induced MI (Roy and Prince, 2012). Reperfusion injury occurs due to ischaemia followed by reintroduction of blood flow to a previously ischaemic tissue, causing additional myocardial damage and cardiac contractile dysfunction (Silambarasan et al, 2015). As oxidative stress is an important contributor to ischaemia/reperfusion (I/R) injury, antioxidants have been considered as a potential therapeutic option (Yang et al, 2013).…”

Section: Protective Role In Cardiovascular Diseasesupporting

confidence: 67%

“…Decreases in the activity of several TCA enzymes were blunted after pre-treatment with SA. SA was also found to reduce H9c2 cardiomyoblast cell injury caused by H 2 O 2 , probably due to its scavenging abilities (Silambarasan et al, 2015). In N ω -nitro-l-arginine methyl ester hydrochloride (l-NAME)-treated rats, SA decreased the mean arterial pressure, along with kidney and liver weights.…”

Section: Protective Role In Cardiovascular Diseasementioning

confidence: 99%

“…Properties of SA Antioxidant activity of SA SA can modulate oxidative stress pathways in the cell and may have potential in treating diabetes and high blood pressure (Roy and Prince, 2012;Silambarasan et al, 2015). Oxidative stress can be defined as an imbalance in the "redox" status of a cell, which occurs when the levels of reactive oxygen species (ROS) and antioxidants are unbalanced (Nordberg and Arner, 2001).…”

Section: Sa and Pca In Human Health And Diseasementioning

confidence: 99%

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Sinapinic and protocatechuic acids found in rapeseed: isolation, characterisation and potential benefits for human health as functional food ingredients

Quinn

Gray

Meaney³

et al. 2017

Irish Journal of Agricultural and Food Research

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Rapeseed is one of the world’s major oilseeds, and rapeseed oil is produced by pressing of the seeds. This process results in the production of a low-economic-value by-product, rapeseed meal, which is commonly used as animal feed. Rapeseed meal is rich in bioactive phenolic compounds, including sinapinic acid (SA) and protocatechuic acid (PCA). Isolation of these bioactive compounds from a by-product of rapeseed oil production is largely in agreement with the current concept of the circular economy and total utilisation of crop harvest using a biorefinery approach. In this review, current information concerning traditional and novel methods to isolate phenolic compounds – including SA and PCA – from rapeseed meal, along with in vitro and in vivo studies concerning the bioactivity of SA and PCA and their associated health effects, is collated. These health effects include anti-inflammatory, anti-cancer, anti-diabetes activities, along with histone deacetylase inhibition and protective cardiovascular, neurological and hepatic effects. The traditional extraction methods include use of solvents and/or enzymes. However, a need for simpler, more efficient methodologies has led to the development of novel extraction processes, including microwave-assisted, ultrasound-assisted, pulsed electric field and high-voltage electrical discharge extraction processes.

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Section: Protective Role In Cardiovascular Diseasesupporting

confidence: 67%

Section: Protective Role In Cardiovascular Diseasementioning

confidence: 99%

Section: Sa and Pca In Human Health And Diseasementioning

confidence: 99%

See 1 more Smart Citation

Sinapinic and protocatechuic acids found in rapeseed: isolation, characterisation and potential benefits for human health as functional food ingredients

Quinn

Gray

Meaney³

et al. 2017

Irish Journal of Agricultural and Food Research

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“…These phenolic acids exhibit a variety of biological activities such as anti‐inflammatory and neuroprotective activities. They also exhibit good antioxidant and prevent lipid peroxidation and mitochondrial dysfunction (Silambarasan et al, ). Iqbal and Fry () reported the release of bioactive compounds from cress seeds as soon as the seeds are imbibed.…”

Section: Resultsmentioning

confidence: 99%

Phenolic acid composition, antioxidant and antimicrobial activities of green gram (vigna radiata) exudate, husk, and germinated seed of different stages

Krishnappa

Basha

Negi

et al. 2017

J Food Process Preserv

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Germinated green gram seeds are widely consumed in the world. During germination, significant volume of exudate is wasted. Total polyphenol, flavonoid and anthocyanin contents in exudate were 80.8 mg GAE/g, 52.42 mg CAE/g and 15.23 µg/g (dw), respectively. In exudate, sinapic acid content was the highest followed by ferulic and gallic acids. Similarly, polyphenol and flavonoid contents in germinated seeds increased, while in husk, it decreased significantly with the germination period, however, anthocyanin content decreased significantly in both. During germination, contents of tannic and gallic acids decreased, while contents of ferulic and sinapic acids increased in both seed and husk. Exudate exhibited potential antioxidant and antibacterial activities. First day germinated seed and husk had the highest antioxidant and antibacterial potential. Thus, exudate and germinated seed extracts can be used as nutraceuticals and used in functional foods. This is the first report on antioxidant and antibacterial properties of green gram exudate. Practical applications Germinated seeds are rich in nutrients and nutraceuitcals. Food industry produces significant amount of germinated green gram and these germinated seeds are consumed as raw as well as after mild processing. After germination, large quantities of liquid as well as husk are obtained. As germinating seeds produce antimicrobial compounds to protect the seedlings, during germination these compounds may leach into exudate. In the present study, we found that green gram exudate and the germinating seed husks possess stronger antioxidant and antibacterial activity against food borne bacteria compared to raw seed. The results suggest that the exudate can be a good source of natural preservatives in food products and it may be an alternative to the use of chemical additives in the food industry.

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“…Mitochondria perform a critical role in the supply of energy to the heart; mitochondrial dysfunction is closely associated with cardiac dysfunction [28]. In order to reveal the underlying mechanisms of the mitochondrial dysfunction in MI, researchers have mainly focused on mitochondrial impairment, especially in mitochondrial enzymes, during MI [29–31].…”

Section: Discussionmentioning

confidence: 99%

Dl-3-n-butylphthalide protects the heart against ischemic injury and H9c2 cardiomyoblasts against oxidative stress: involvement of mitochondrial function and biogenesis

Tian

Yang

et al. 2017

J Biomed Sci

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BackgroundMyocardial infarction (MI) is an acute and fatal condition that threatens human health. Dl-3-n-butylphthalide (NBP) has been used for the treatment of acute ischemic stroke. Mitochondria may play a protective role in MI injury. However, there are few reports on the cardioprotective effect of NBP or the potential mitochondrial mechanism for the NBP-induced protection against cardiac ischemia injury. We investigated the therapeutic effects of NBP in an in vivo MI model and an in vitro oxidative stress model, as well as the potential mitochondrial mechanism.MethodsThis study comprised two different experiments. The aim of experiment 1 was to determine the protective effects of NBP on MI and the underlying mechanisms in vivo. In part 1, myocardial infarct size was measured by staining with 2,3,5-triphenyltetrazoliumchloride (TTC). Myocardial enzymes and mitochondrial enzymes were assayed. The aim of experiment 2 was to investigate the role of NBP in H2O2-induced myocardial ischemic injury in H9c2 cells and to determine the potential mechanism. In part 2, H9c2 cell viability was evaluated. ROS levels, mitochondrial morphology, and mitochondrial membrane potential of H9c2 cells were measured. ATP levels were evaluated using an assay kit; mitochondrial DNA (mtDNA), the expressions of NRF-1 and TFAM, and mitochondrial biogenesis factors were determined.ResultsNBP treatment significantly reduced the infarct ratio, as observed by TTC staining, decreased serum myocardial enzymes in MI, and restored heart mitochondrial enzymes (isocitrate dehydrogenase (ICDH), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), and a-ketoglutarate dehydrogenase (a-KGDH) activities after MI. Moreover, in in vitro studies, NBP significantly increased the viability of H9c2 cells in a dose-dependent manner, reduced cell apoptosis, protected mitochondrial functions, elevated the cellular ATP levels, and promoted H2O2-induced mitochondrial biogenesis in H9c2 cardiomyoblasts.ConclusionCollectively, the results from both the in vivo and in vitro experiments suggested that NBP exerted a cardioprotective effect on cardiac ischemic injury via the regulation of mitochondrial function and biogenesis.

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Sinapic acid protects heart against ischemia/reperfusion injury and H9c2 cardiomyoblast cells against oxidative stress

Cited by 46 publications

References 42 publications

Sinapinic and protocatechuic acids found in rapeseed: isolation, characterisation and potential benefits for human health as functional food ingredients

Sinapinic and protocatechuic acids found in rapeseed: isolation, characterisation and potential benefits for human health as functional food ingredients

Phenolic acid composition, antioxidant and antimicrobial activities of green gram (vigna radiata) exudate, husk, and germinated seed of different stages

Dl-3-n-butylphthalide protects the heart against ischemic injury and H9c2 cardiomyoblasts against oxidative stress: involvement of mitochondrial function and biogenesis

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