Sine oculis homeobox homolog 1 promotes α5β1-mediated invasive migration and metastasis of cervical cancer cells

Liu, Dan; Zhang, Xiaoxue; Wan, Dongyi; Xi, Bixin; Ma, Ding; Wang, Hui; Gao, Qinglei

doi:10.1016/j.bbrc.2014.03.002

Cited by 23 publications

(20 citation statements)

References 27 publications

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“…RFP/luciferase-expressing tumor cells were prepared as we described previously (31). Siha cells and C33a cells were then transfected with pcDNA3.1 control plasmid or pcDNA3.1-SIX1 expression plasmid (a kind gift from Kong-Ming Wu, Thomas Jefferson University, Philadelphia, PA) using Lipofectamine 2000 (Invitrogen) and selected with G418.…”

Section: Cell Transfectionmentioning

confidence: 99%

“…Tissue sections were subjected to immunohistochemical (IHC) analysis as described previously (32). For semiquantitative evaluation of protein level in tissue, an immunoreactivity scoring system HSCORE was used (28,31). HSCORE 2.0 was classified as a low protein level, and HSCORE > 2.0 was classified as a high protein level.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…The relative quantity of mRNA was determined by real-time RT-PCR as described previously (31). GAPDH and EEF1A1 were chosen as reference genes, which were reported as the most reliable combination in cervical cancer (37).…”

Section: Assay Of Gene Expression By Real-time Rt-pcrmentioning

confidence: 99%

“…Flow cytometry was performed as described previously (31). The expression index was calculated using the formula: mean fluorescence Â percentage of positively stained cells (31,38).…”

Section: Flow Cytometrymentioning

confidence: 99%

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SIX1 Promotes Tumor Lymphangiogenesis by Coordinating TGFβ Signals That Increase Expression of VEGF-C

Liu

Zhang

et al. 2014

Cancer Research

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Section: Cell Transfectionmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Assay Of Gene Expression By Real-time Rt-pcrmentioning

confidence: 99%

“…Flow cytometry was performed as described previously (31). The expression index was calculated using the formula: mean fluorescence Â percentage of positively stained cells (31,38).…”

Section: Flow Cytometrymentioning

confidence: 99%

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SIX1 Promotes Tumor Lymphangiogenesis by Coordinating TGFβ Signals That Increase Expression of VEGF-C

Liu

Zhang

et al. 2014

Cancer Research

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“…The percentage of positive cells was defined as the percentage of cells in the gate, which was set to exclude $99% of isotype control cells. The expression index of SEMA4C was calculated using the formula: mean fluorescence Â percentage of positively stained cells (22,23).…”

Section: Flow Cytometric Analysismentioning

confidence: 99%

Tumor-associated Lymphatic Endothelial Cells Promote Lymphatic Metastasis By Highly Expressing and Secreting SEMA4C

Wei

Yang

Liu

et al. 2017

Clinical Cancer Research

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Purpose: Lymphatic vessels are mainly regarded as passive conduits for the dissemination of cancer cells. In this study, we investigate whether and how the tumor-associated lymphatic vessels may play an active role in tumor metastasis.Experimental Design: In situ laser capture microdissection of lymphatic vessels followed by cDNA microarray analysis was used to determine the expression profiling of lymphatic endothelial cells (LEC). Gene expression levels and activity of signaling pathways were measured by real-time RT-PCR, ELISA, or immunoblotting. Lymphangiogenesis was assessed by IHC. Lymph node metastasis was measured using fluorescence imaging. The effects of SEMA4C on lymphangiogenesis in vitro were evaluated using migration assay and tube-formation assay of LECs.Results: Tumor-associated LECs are molecularly and functionally different from their normal counterparts. In addition to expressing high levels of membrane-bound SEMA4C, tumorassociated LECs also produced soluble SEMA4C (sSEMA4C). Increased serum sSEMA4C was detected in patients with breast cancer and cervical cancer. Patients with metastasis had much higher levels of serum sSEMA4C. sSEMA4C promoted lymphangiogenesis by activating PlexinB2-ERBB2 signaling in LECs, and promoted the proliferation and migration of tumor cells by activating PlexinB2-MET signaling, thus promoting lymphatic metastasis. Although the SEMA4C signaling pathways differ between LECs and tumor cells, RHOA activation was necessary for the effects of SEMA4C in both types of cells.Conclusions: Tumor-associated LECs produce sSEMA4C to promote lymphatic metastasis of tumors. Our results suggest that SEMA4C and RHOA might be potential therapeutic targets, and that higher serum sSEMA4C could be a marker for breast cancer and cervical cancer.

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Cytotoxic Activity of A New Isoform l-Amino Acid Oxidase (Balt-LAAO-II) From Bothrops alternatus (Urutu) Snake Venom in Human Leukemic HL60 Cells

Heleno,

Nowill,

de Carvalho

et al. 2023

Int J Pept Res Ther

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Sine oculis homeobox homolog 1 promotes α5β1-mediated invasive migration and metastasis of cervical cancer cells

Cited by 23 publications

References 27 publications

SIX1 Promotes Tumor Lymphangiogenesis by Coordinating TGFβ Signals That Increase Expression of VEGF-C

SIX1 Promotes Tumor Lymphangiogenesis by Coordinating TGFβ Signals That Increase Expression of VEGF-C

Tumor-associated Lymphatic Endothelial Cells Promote Lymphatic Metastasis By Highly Expressing and Secreting SEMA4C

Cytotoxic Activity of A New Isoform l-Amino Acid Oxidase (Balt-LAAO-II) From Bothrops alternatus (Urutu) Snake Venom in Human Leukemic HL60 Cells

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